Direct determination of pindolol enantiomers in human serum by column-switching LC-MS/MS using a phenylcarbamate-β-cyclodextrin chiral column

Motoyama, Akira; Suzuki, Ayako; Shirota, Osamu; Namba, Ryujiro

doi:10.1016/s0731-7085(01)00631-8

Cited by 21 publications

(20 citation statements)

References 21 publications

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“…Other important applications of CDs involve: supramolecular carrier in organometallic reactions [17] and pharmaceutical applications for drug release [18,19]. To produce HPLC columns allowing chiral enantiomers separation, -cyclodextrins are used [20].…”

Section: Cyclodextrins (Cds)mentioning

confidence: 99%

From Zeolite to Host-Guest Nanocomposite Materials

Salavati‐Niasari¹,

Mohandes²

2011

Advances in Diverse Industrial Applications of Nanocomposites

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Section: Cyclodextrins (Cds)mentioning

confidence: 99%

From Zeolite to Host-Guest Nanocomposite Materials

Salavati‐Niasari¹,

Mohandes²

2011

Advances in Diverse Industrial Applications of Nanocomposites

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“…Their results showed that no matrix effects and no co-eluting peaks interfere with the enantiomeric quantification of baclofen, a muscle relaxant. Motoyama and colleagues [45] used a simple centrifugation and filtration step before injecting the serum sample onto a pre-column with a strong cation exchange stationary phase. The purified eluent was then injected to the chiral column using a column-switching system.…”

Section: Plasma and Serum Samplementioning

confidence: 99%

Liquid separation techniques coupled with mass spectrometry for chiral analysis of pharmaceuticals compounds and their metabolites in biological fluids

Erny

Cifuentes

2006

Journal of Pharmaceutical and Biomedical Analysis

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Determination of the chiral composition of drugs is nowadays a key step in order to determine purity, activity, bioavailability, biodegradation, etc, of pharmaceuticals. In this manuscript, works published for the last 5 years on the analysis of chiral drugs by liquid separation techniques coupled with mass spectrometry are reviewed. Namely, chiral analysis of pharmaceuticals including e.g., antiinflammatories, antihypertensives, relaxants, etc, by liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry are included. The importance and interest of the analysis of the enantiomers of the active compound and its metabolites in different biological fluids (plasma, urine, cerebrospinal fluid, etc) are also discussed.

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“…Orazio et al, used vancomycin fused silica capillary for the enantiomeric resolution of pindolol [32]. The enantioselective determination of pindolol in human serum or urine has also been achieved by LC-MS-MS after enantiomer resolution on a phenylcarbamate-β-cyclodextrin column [33]. Recently, pindolol enantiomers in amniotic fluid and breast milk using Chiralpak AD column with a fluorescence detection has been reported [34].…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic Development of a Sensitive and Stereoselective HPLC Method for the Analysis of Pindolol in Plasma and Pharmaceutical Products Using a Chiralpak IB Column and Fluorescence Detection

Hefnawy¹,

Kassem²,

Abdine³

et al. 2009

Sci. Pharm.

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A sensitive and stereoselective HPLC method has been developed for the simultaneous determination of pindolol enantiomers in plasma and pharmaceutical products. Enantiomeric resolution was achieved on a cellulose tris(3,5-dimethylphenylcarbamate) immobilized onto a 5 µm spherical porous silica chiral stationary phase (CSP) known as Chiralpak IB with fluorescence detection set at 266 nm for excitation and 308 nm for emission. The mobile phase consists of n-hexane-isopropanol-triethylamine, (50:50:0.5), (v/v/v) has been used at a flow rate of 1.0 ml/min. The method was highly specific where other coformulated drugs such as clopamide and isosorbide did not interfere. The stability of pindolol enantiomers under different degrees of temperature was studied. The method validated for its linearity, accuracy, precision and robustness. There was no significant difference (p>0.05) between inter-and intra-day studies for each enantiomers which confirmed the reproducibility of the assay method. Preliminary data suggest that S-(−)-pindolol induces less bradycardia but more sedation and central nervous system depression than racemic pindolol.

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Direct determination of pindolol enantiomers in human serum by column-switching LC-MS/MS using a phenylcarbamate-β-cyclodextrin chiral column

Cited by 21 publications

References 21 publications

From Zeolite to Host-Guest Nanocomposite Materials

From Zeolite to Host-Guest Nanocomposite Materials

Liquid separation techniques coupled with mass spectrometry for chiral analysis of pharmaceuticals compounds and their metabolites in biological fluids

Pharmacokinetic Development of a Sensitive and Stereoselective HPLC Method for the Analysis of Pindolol in Plasma and Pharmaceutical Products Using a Chiralpak IB Column and Fluorescence Detection

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