Abstract:A flow-injection system with sample and reagent addition by the synchronous merging zones approach for calcium determination in milk by flame AAS is proposed. Main parameters were optimized using a factorial design with central point. The optimum conditions were 2.5% (m/v) for La concentration, 8 mL min-1 for the carrier flow-rate, 20 cm for coiled reactor and 250 ìL for sample volume. Different sample preparation procedures were evaluated such as dilution in water or acid and microwave-assisted decomposition … Show more
“…Furthermore, the use of CE methods which include simplicity, versatility, reliability, high sensitivity and low sample consumption, result more convenient in compare with sophisticated methods as FAAS [49][50] or FAES [24]. Even though very low levels for detection and quantitation limits are obtained, these methods result more expensive and highly complex.…”
Section: Ca 2+ Quantitation: Advantages Over Other Reported Methodsmentioning
This work presents the development and validation of a capillary zone electrophoresis method for calcium ions release quantitation from composite biomaterials with potential application in bone tissue engineering.Calcium ions quantitation was made using a fused silica capillary (40 cm, 75 µm ID) and background electrolyte containing 5 mM imidazole, 6 mM α-hydroxyisobutyric acid, 1 mM 1,4,7,10,13,16-hexaoxacyclooctadecane and 20 % w/v methanol, at pH 4.5. Indirect UV-detection mode at 214 nm was performed. The separations were achieved using normal polarity at 6 kV, a cartridge temperature of 25 °C and 0.5 psi for 5 s for sample introduction. Parameters of validation such as specificity, linearity, limit of detection and quantitation, accuracy, precision and robustness were evaluated according to the International Conference on Harmonization guidelines. The new developed method resulted to be suitable for calcium determination from composite biomaterials for potential application in bone tissue engineering.
“…Furthermore, the use of CE methods which include simplicity, versatility, reliability, high sensitivity and low sample consumption, result more convenient in compare with sophisticated methods as FAAS [49][50] or FAES [24]. Even though very low levels for detection and quantitation limits are obtained, these methods result more expensive and highly complex.…”
Section: Ca 2+ Quantitation: Advantages Over Other Reported Methodsmentioning
This work presents the development and validation of a capillary zone electrophoresis method for calcium ions release quantitation from composite biomaterials with potential application in bone tissue engineering.Calcium ions quantitation was made using a fused silica capillary (40 cm, 75 µm ID) and background electrolyte containing 5 mM imidazole, 6 mM α-hydroxyisobutyric acid, 1 mM 1,4,7,10,13,16-hexaoxacyclooctadecane and 20 % w/v methanol, at pH 4.5. Indirect UV-detection mode at 214 nm was performed. The separations were achieved using normal polarity at 6 kV, a cartridge temperature of 25 °C and 0.5 psi for 5 s for sample introduction. Parameters of validation such as specificity, linearity, limit of detection and quantitation, accuracy, precision and robustness were evaluated according to the International Conference on Harmonization guidelines. The new developed method resulted to be suitable for calcium determination from composite biomaterials for potential application in bone tissue engineering.
“…The preparation of sample destruction was based on a method used by Moreno-Torrese et al (2000), Zafar et al (2003), Ayed and Thannoun (2006) and Petrovich et al (2007). On solid samples such as feed, milk and feces, drying and ashing at 550 o C were done until samples become white ash.…”
Section: Calcium Analysis and Its Absorptionmentioning
Calcium absorption of standard milk is influenced by types of fat, other than types of calcium salts, vitamin D, PTH hormone, etc. However, calcium absorption on different fat content, particularly on milk, has not been reported. The purpose of this study was to determine the effect of the fat content of milk on the absorption of calcium carbonate and calcium phosphate mixture in rats. Experiments were conducted using 24 male Sprague Dawley rats fed with low-fat milk (0.5% fat), high-fat milk (26% fat), standard milk (8% fat) and without milk for three weeks. Amount of feed consumed, feces and urine samples were collected to determine the amount of absorbed calcium for five days in the third week. Calcium content in the samples was analyzed using AAS (Atomic Absorption Spectrophotometry). The results showed that there were no difference in calcium carbonate and calcium phosphate absorption among rats fed with low-fat milk, high-fat milk and standard milk. The calcium absorption level from milk reached an average of around 98.67±0.22%. The absorption level of calcium carbonate and calcium phosphate mixture from milk was higher than control rats that were not fed with any milk. Fat content in milk did not affect the absorption of calcium carbonate and calcium phosphate mixture, but the milk consumption could increase the calcium absorption, compared to control.
“…Calcium is an essential macronutrient for humans, which represents approximately 2% of body weight in an adult person. This element has mainly a structural function in bones and teeth, and also to regulate many vital biological functions [8]. The iron content of milk is of physiological interest.…”
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