Direct detection of Yersinia pestis from the infected animal specimens by a fiber optic biosensor

Wei, Hua; Zhao, Yuchao; Bi, Yujing; Liu, Haihong; Guo, Zhaobiao; Song, Yajun; Zhai, Jing; Huang, Huijie; Yang, Ruifu

doi:10.1016/j.snb.2006.08.010

Cited by 18 publications

(13 citation statements)

References 16 publications

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“…The capsule-like fraction 1 (F1) antigen expressed by Y. pestis is a known specific marker for identified the bacteria; therefore, the detection of F1 is important for Y. pestis recognition [ 22 ]. There were many highly sensitive immunological and biochemical assays to detect F1 antigen, such as the indirect hemagglutination assay (IHA, the gold standard in detection of Y. pestis ) [ 23 ], polymerase chain reaction (PCR) analysis [ 24 ], enzyme-linked immunosorbent assays (ELISAs) [ 25 , 26 ], the fiber optic biosensor measurement to fluorescence antibody staining [ 27 , 28 ], and radioimmune precipitation test [ 29 ]. In China, we used IHA, RIHA, and PCR to detect F1 antigen.…”

Section: Discussionmentioning

confidence: 99%

Plague in China 2014—All sporadic case report of pneumonic plague

Li²,

Shao

et al. 2016

BMC Infect Dis

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BackgroundYersinia pestis is the pathogen of the plague and caused three pandemics worldwide. Pneumonic plague is rarer than bubonic and septicemic plague. We report detailed clinical and pathogenic data for all the three sporadic cases of pneumonic plagues in China in 2014.Case presentationAll the three patients are herders in Gansu province of China. They were all infected by Yersinia pestis and displayed in the form of pneumonic plague respectively without related. We tested patient specimens from the upper (nasopharyngeal swabs) or the lower (sputum) respiratory tract and whole blood, plasma, and serum specimens for Yersinia pestis. All patients had fever, cough and dyspnea, and for patient 2 and 3, unconscious. Respiratory symptoms were predominant with acute respiratory failure. The chest X-ray showed signs consistent with necrotizing inflammation with multiple lobar involvements. Despite emergency treatment, all patients died of refractory multiple organ failure within 24 h after admission to hospital. All the contacts were quarantined immediately and there were no secondary cases.ConclusionsNowadays, the plague is epidemic in animals and can infect people who contact with the infected animals which may cause an epidemic in human. We think dogs maybe an intermediate vector for plague and as a source of risk for humans who are exposed to pet animals or who work professionally with canines. If a patient has been exposed to a risk factor and has fever and dyspnea, plague should be considered. People who had contact with a confirmed case should be isolated and investigated for F1 antigen analysis and receive post-exposure preventive treatment. A vaccination strategy might be useful for individuals who are occupationally exposed in areas where endemically infected reservoirs of plague-infected small mammals co-exist.

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Section: Discussionmentioning

confidence: 99%

Plague in China 2014—All sporadic case report of pneumonic plague

Li²,

Shao

et al. 2016

BMC Infect Dis

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“…Likewise, sensitivities of well less than 1 cfu or pfu/g food are desired for bacterial or viral targets to avoid infection (or intoxication if the microbes are toxigenic). Enzyme-based assay using pH sensitive dye; kinetic measurements Ramanathan and Simonian (2007) Y. pestis cells, F1 antigen Fiber-optic biosensor (multispecific) Detected in liver, spleen, lung of infected mice and Wei, Zhao, et al (2007) …”

Section: Sample Preparationmentioning

confidence: 99%

Total internal reflection fluorescence (TIRF) array biosensors for biothreat agents for food safety and food defense

Taitt¹,

North²

2015

High Throughput Screening for Food Safety Assessment

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“…Y. pestis was often detected by bacterial isolation and microscopy observation, 9 phage lysis assay, 10 , 11 ELISA assays based on the detection of F1 antigen and antibodies against Y. pestis , 12 , 13 , 14 , 15 conventional PCR assays, 16 , 17 , 18 real-time quantitative PCR assays, 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 biosensors based on fiber-optic or upconverting phosphor technology, 29 , 30 , 31 solid-phase radioimmunoassay based on radiolabeled monoclonal antibody for the detection of plague antigen. 32 All these methods are playing an important role in the diagnosis of plague, but these methods either are time-consuming and laborious, or require expensive equipment and personnel with a high level of technical expertise.…”

Section: Introductionmentioning

confidence: 99%

Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA

Feng

Zhou

Fan

et al. 2018

Brazilian Journal of Microbiology

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We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. The sensitivity of LAMP or PCR was 2.3 or 23 CFU for pure culture, whereas 2.3 × 104 or 2.3 × 106 CFU for simulated spleen and lung samples. For simulated liver samples, the sensitivity of LAMP was 2.3 × 106 CFU, but PCR was negative at the level of 2.3 × 107 CFU. After simulated spleen and lung samples were treated with magnetic beads, the sensitivity of LAMP or PCR was 2.3 × 103 or 2.3 × 106 CFU, whereas 2.3 × 105 or 2.3 × 107 CFU for magnetic bead-treated liver samples. These results indicated that some components in the tissues could inhibit LAMP and PCR, and liver tissue samples had a stronger inhibition to LAMP and PCR than spleen and lung tissue samples. LAMP has a higher sensitivity than PCR, and magnetic bead capture of DNAs could remarkably increase the sensitivity of LAMP. LAMP is a simple, rapid and sensitive assay suitable for application in the field or poverty areas.

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Direct detection of Yersinia pestis from the infected animal specimens by a fiber optic biosensor

Cited by 18 publications

References 16 publications

Plague in China 2014—All sporadic case report of pneumonic plague

Plague in China 2014—All sporadic case report of pneumonic plague

Total internal reflection fluorescence (TIRF) array biosensors for biothreat agents for food safety and food defense

Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA

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