Direct detection of Listeria monocytogenes DNA amplification products with quartz crystal microbalances at elevated temperatures

Wachiralurpan, Sirirat; Chansiri, Kosum; Lieberzeit, Peter A.

doi:10.1016/j.snb.2020.127678

Cited by 18 publications

(15 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inserting the respective values into the Sauerbrey’s equation at the irreversible frequency shift (−342 Hz with noise being ~5 Hz) led to estimate the mass change after immobilization on the QCM electrode as approximately 300 ng. According to previous work [ 9 ], the molecular ratio in the solution is roughly 1 molecule ssDNA probe per 55 molecules L-cysteine. The hybridization sensor functionalized with thiolated single-stranded DNA probes thus contains approximately 1.8 × 10 13 DNA molecules (MW = 9982.52 g/mol) (see Supplementary file S1 ) when co-immobilizing them with L-cysteine.…”

Section: Resultsmentioning

confidence: 99%

“…Details of the QCM sensor set-up and fabrication of the sensing electrode can be found in a previously published paper [ 9 ], which demonstrates all the necessary optimization experiments to achieve ideal self-assembled monolayers containing an oligonucleotide capture probe. In brief, we printed the electrode structures on both sides of AT-cut single crystal quartz plates with 13.8 mm diameter and 168 µm thickness with brilliant gold paste to yield QCM.…”

Section: Methodsmentioning

confidence: 99%

“…Immobilizing single-strand capture DNA on electronic devices and circuits requires chemical modification that leads to the desired recognition structures (i.e., self-assembled monolayers) [7,8]. A recent study has shown that using a spacer between immobilized DNA strands, such as L-cysteine, increases sensitivity, Biosensors 2021, 11, 308 2 of 14 because it makes the capture probes on the surface sterically more accessible [9] to the analyte. Herein, we demonstrate how the results of that paper can be carried forward to ensure amplification and measurements in one step simultaneously, i.e., in-situ.…”

Section: Introductionmentioning

confidence: 99%

“…For that purpose, we use a recently developed method to provide in-situ measurement of DNA amplification [ 9 ], relying on QCM measurements ( Scheme 1 ). That paper demonstrates the method for achieving such measurements in principle and strongly focuses on the optimization steps required to ensure optimal hybridization between the immobilized oligonucleotide and a target single-strand DNA.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In-Situ Monitoring of Real-Time Loop-Mediated Isothermal Amplification with QCM: Detecting Listeria monocytogenes

et al. 2021

Self Cite

View full text Add to dashboard Cite

Functionalized DNA sequences are promising sensing elements to combine with transducers for bio-sensing specific target microbes. As an application example, this paper demonstrates in situ detection of loop-mediated isothermal amplification products by hybridizing them with thiolated-ssDNA covalently anchored on the electrodes of a quartz crystal microbalance (QCM). Such hybridization leads to a frequency signal, which is suitable for monitoring real-time LAMP amplification based on mass-sensing: it detects interactions between the complementary nucleobases of LAMP products in solution and the thiolated-ssDNA probe sequence on the gold surface. Target DNA LAMP products cause irreversible frequency shifts on the QCM surfaces during hybridization in the kHz range, which result from both changes in mass and charge on the electrode surface. In order to confirm the LAMP assay working in the QCM sensing system at elevated temperature, the sky blue of positive LAMP products solution was achieved by using the Hydroxy Naphthol Blue (HNB) and agarose gel electrophoresis. Since on-QCM sensing of DNA hybridization leads to irreversible sensor responses, this work shows characterization by X-ray photoelectron spectroscopy (XPS) core spectra of S2p, N1s, Mg1s, P2p and C1s. XPS results confirmed that indeed both DNA and by-products of LAMP attached to the surface. Listeria monocytogenes DNA served to study in-situ detection of amplified LAMP products on DNA-functionalized surfaces.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In-Situ Monitoring of Real-Time Loop-Mediated Isothermal Amplification with QCM: Detecting Listeria monocytogenes

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some conventional technologies including enzyme-linked immunosorbent assay, polymerase chain reaction assay, surface plasmon resonance, quartz crystal microbalance immunosensor and fiber-optic immunosensor have been developed to detect LM. 3,[7][8][9] It's no doubt that these methods are very important and can largely fit the demands for detecting LM. However, they usually suffered from some deficiency such as labor-intensive, time-consuming, complex pretreatments or expensive equipments.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Electrochemical Immunosensing for Listeria Monocytogenes Based on 3,4,9,10-Perylene Tetracarboxylic Acid/ Graphene Ribbons as a Sensing Platform and Ferrocene/Gold Nanoparticles as an Amplifier

Jiang

Ding

et al. 2021

ANAL. SCI.

View full text Add to dashboard Cite

As a gram-positive foodborne pathogen, Listeria monocytogenes (LM) can cause many serious diseases to the human health coupled with high mortality rates, thus constructing effective method to detect LM is of great significance. Herein, a novel sandwich-type electrochemical immunosensor was proposed for LM by introducing 3,4,9,10-perylene tetracarboxylic acid/graphene ribbons (PTCA/GNR) nanohybrids as sensing platform and ferrocene/gold nanoparticles (Fc/Au NPs) as signal amplifier. The high conductivity and large surface area of GNR can increase the immobilizing amount of primary antibody (PAb) and enhance the electron transport rate, while Au NPs can carry secondary antibodies (SAb) and Fc derivative (Fc-SH) to form SAb-Au NPs-Fc signal amplifier. Through using Fc molecules as signal probe, its peak current can appear and increase varied from the LM concentrations, hence a highly sensitive sandwich-type immunosensor was constructed: the linear range is wide from 10 to 2×10 4 CFU mL -1 and the limit of detection is low to 6 CFU mL -1 . Furthermore, the specificity of the immunosensor was also studied and a satisfactory result was obtained.

show abstract

Principles and Recent Advances in Biosensors for Pathogens Detection

et al. 2021

View full text Add to dashboard Cite

Foodborne and waterborne diseases caused by pathogens pose a serious threat to human life, and the detection of such pathogens in food, water, and beverages has gained paramount importance in view of the increasing number of pathogenic diseases in recent times. Standard and traditional analytical techniques employed for the recognition of deadly pathogens, including polymerase chain reaction‐based techniques, immunology‐based assays, culture and colony counting techniques, require several hours or perhaps even a few days for the results. All these techniques, despite being quite sensitive, are time‐intensive. Therefore, several researchers are focusing on the development of rapid pathogen detection techniques. Although the most advanced biosensing technologies exhibit potential approaches, significant research and testing is an urgent need to design innovative biosensors. Novel bioanalytical approaches for the recognition and quantification of target pathogens are being designed and developed to enhance the characteristics of biosensors, including rapid response, selectivity, and sensitivity. In this context, we not only provide an overview of the types of biorecognition elements employed for the detection of pathogens, but also discuss in detail the traditional approaches, biomolecular techniques, the latest advances in the detection and quantification of foodborne and waterborne pathogens, with particular emphasis on biosensors.

show abstract

Direct detection of Listeria monocytogenes DNA amplification products with quartz crystal microbalances at elevated temperatures

Cited by 18 publications

References 42 publications

In-Situ Monitoring of Real-Time Loop-Mediated Isothermal Amplification with QCM: Detecting Listeria monocytogenes

In-Situ Monitoring of Real-Time Loop-Mediated Isothermal Amplification with QCM: Detecting Listeria monocytogenes

Highly Sensitive Electrochemical Immunosensing for Listeria Monocytogenes Based on 3,4,9,10-Perylene Tetracarboxylic Acid/ Graphene Ribbons as a Sensing Platform and Ferrocene/Gold Nanoparticles as an Amplifier

Principles and Recent Advances in Biosensors for Pathogens Detection

Contact Info

Product

Resources

About