Direct detection of DNA using electrical double layer gated high electron mobility transistor in high ionic strength solution with high sensitivity and specificity

Chen, Yen Wen; Tai, Tse Yu; Hsu, Cheng-Wei; Sarangadharan, Indu; Pulikkathodi, Anil Kumar; Wang, Hsin Li; Sukesan, Revathi; Lee, Geng Yen; Chyi, Jen Inn; Chen, Chih Chen; Lee, Gwo‐Bin; Wang, Yu Lin

doi:10.1016/j.snb.2018.05.119

Cited by 20 publications

(13 citation statements)

References 24 publications

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“…This demonstrates the specificity of the microRNA-195 probe. In a previous study [28], 6 bp-mismatched DNA was used to verify the specificity of a microRNA probe; the output charge increased 13 and 6% for target and off-target DNA sequences, respectively, inferior to the 124 and 4% values, respectively, documented herein.…”

Section: B Specificity Testsmentioning

confidence: 49%

A CMOS-Based Capacitive Biosensor for Detection of a Breast Cancer MicroRNA Biomarker

Kuo

Chen

Huang

et al. 2020

IEEE Open J. Nanotechnol.

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Breast cancer ranks among the most common cancers worldwide and can be lethal when not diagnosed early due to the high probability of metastasis. Herein a complementary metal-oxide-semiconductor (CMOS)-based capacitive nano-biosensor was developed to quickly and accurately quantify the concentration of an early-stage breast cancer diagnostic marker, microRNA-195, in blood. The microRNA probe was immobilized on optimized inter-digitated electrodes (IDE), and CMOS-sensing circuits detected the probe-analyte reaction at microRNA-195 concentrations as low as 0.617 fM. This high sensitivity could be due to the monolithically integrated nature of the circuits, for which "parasitic" effects on the capacitive sensors were markedly low. The CMOS-based capacitive nano-sensor may be promising for early diagnosis of breast cancer.

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Section: B Specificity Testsmentioning

confidence: 49%

A CMOS-Based Capacitive Biosensor for Detection of a Breast Cancer MicroRNA Biomarker

Kuo

Chen

Huang

et al. 2020

IEEE Open J. Nanotechnol.

Self Cite

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“…Thus, any changes in the C s will regulate a potential drop in the device dielectric medium, hence resulting in the current output response. This is due to the charge redistribution changes at the EDL [ 141 ]. In fact, the C s can change in several states; (1) when the ionic strength of the test medium changes, (2) when the surface area of the gate electrode is functionalized, and (3) when receptor–ligand binding modifies the electrostatic interaction at the gate electrode’s EDL [ 142 ].…”

Section: Electric Double-layers (Edl Hemt)mentioning

confidence: 99%

“…This high ionic strength solution includes urine, whole blood, and serum or physiological solutions. Detection of biosensors through changes in charge distribution and potential gradients is limited only within the λ D [ 141 ]. Typical physiological samples have small dimensions (about 0.7–2.2 nm) in comparison to the size of larger receptor molecules like antibodies (about 10–15 nm).…”

Section: Challenges and Opportunities Of Heterojunction-based Hemtmentioning

confidence: 99%

Status and Prospects of Heterojunction-Based HEMT for Next-Generation Biosensors

et al. 2023

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High electron mobility transistor (HEMT) biosensors hold great potential for realizing label-free, real-time, and direct detection. Owing to their unique properties of two-dimensional electron gas (2DEG), HEMT biosensors have the ability to amplify current changes pertinent to potential changes with the introduction of any biomolecules, making them highly surface charge sensitive. This review discusses the recent advances in the use of AlGaN/GaN and AlGaAs/GaAs HEMT as biosensors in the context of different gate architectures. We describe the fundamental mechanisms underlying their operational functions, giving insight into crucial experiments as well as the necessary analysis and validation of data. Surface functionalization and biorecognition integrated into the HEMT gate structures, including self-assembly strategies, are also presented in this review, with relevant and promising applications discussed for ultra-sensitive biosensors. Obstacles and opportunities for possible optimization are also surveyed. Conclusively, future prospects for further development and applications are discussed. This review is instructive for researchers who are new to this field as well as being informative for those who work in related fields.

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“…However, an obstacle remains when it comes to the practical application of AlGaN/GaN HEMT biosensors for real-time diagnosis in physiological solu-tions: the Debye screening in solutions of high ionic strength negatively influences the detection of target analytes [11]. To solve this problem, Wang's group proposed an electrical double layer (EDL) gated AlGaN/ GaN HEMT structure where the reconstruction of EDL helps minimize the influence of the Debye screening effects, which could directly detect analytes in 1x phosphate buffered saline (PBS) or human sera [17][18]. Li's group introduced an analytical platform combining the enzyme-linked immunosorbent assay (ELISA) strategy and an AlGaN/GaN HEMT biosensor, named e-ELISA, which enabled the detection of target analytes quickly and sensitively without Debye screening [19].…”

Section: Introductionmentioning

confidence: 99%

Direct Protein Detection in Solutions of High Ionic Strength using Polyethylene Glycol‐modified AlGaN/GaN High Electron Mobility Transistors

Diao

Zhang

et al. 2022

Electroanalysis

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Herein, we proposed a simple 3‐(aminopropyl) triethoxysilane/polyethylene glycol (APTES/PEG)‐AlGaN/GaN high electron mobility transistors (HEMT) biosensor that enabled direct detection in high ionic strength solutions. Unlike previous modification strategies, the PEG‐modified on the GaN surface established a stable screening system by forming a porous biopolymer layer, which can overcome Debye shielding. In our work, the APTES/PEG modification enabled immunoglobulin G (IgG) to be easily detected in solutions with concentrations of up to 30 mM (3x) of phosphate buffered saline (PBS), indicating that the APTES/PEG modification strategy had the potential for direct detection of biomolecules in physiological solutions, which provided a new method for bio‐surface modification on GaN and advanced the practical application of AlGaN/GaN HEMT biosensors.

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Direct detection of DNA using electrical double layer gated high electron mobility transistor in high ionic strength solution with high sensitivity and specificity

Cited by 20 publications

References 24 publications

A CMOS-Based Capacitive Biosensor for Detection of a Breast Cancer MicroRNA Biomarker

A CMOS-Based Capacitive Biosensor for Detection of a Breast Cancer MicroRNA Biomarker

Status and Prospects of Heterojunction-Based HEMT for Next-Generation Biosensors

Direct Protein Detection in Solutions of High Ionic Strength using Polyethylene Glycol‐modified AlGaN/GaN High Electron Mobility Transistors

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