Direct conversion of chicory flour into l(+)-lactic acid by the highly effective inulinase producer Lactobacillus paracasei DSM 23505

Petrova, Penka; Velikova, Petya; Popova, Luiza; Petrov, Kaloyan

doi:10.1016/j.biortech.2015.03.077

Cited by 36 publications

(16 citation statements)

References 17 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The subcellular localization of this protein is predicted to be Ͼ99% extracellular. The protein sequence showed 97% identity to ␤-fructosidases and sucrose-6-phosphorylases present in various strains of L. paracasei and L. casei and around 30% identity to exoinulinases from Bacteroides species and Bacillus subtilis (22,23). To characterize the activity of this putative GH32 enzyme, we cloned the predicted catalytic domain (residues 188 to 739) into the pET15bLIC vector which added an N-terminal 6-His tag (called LpGH32).…”

Section: Resultsmentioning

confidence: 99%

Cross-Feeding among Probiotic Bacterial Strains on Prebiotic Inulin Involves the Extracellular exo -Inulinase of Lactobacillus paracasei Strain W20

Böger

Bueren

Dijkhuizen

2018

Appl Environ Microbiol

View full text Add to dashboard Cite

Probiotic gut bacteria employ specific metabolic pathways to degrade dietary carbohydrates beyond the capabilities of their human host. Here, we report how individual commercial probiotic strains degrade prebiotic (inulin type) fructans. First, a structural analysis of commercial fructose oligosaccharide-inulin samples was performed. These β-(2-1)-fructans differ in termination by either glucose (GF) or fructose (FF) residues, with a broad variation in the degrees of polymerization (DPs). The growth of individual probiotic bacteria on short-chain inulin (sc-inulin) (Frutafit CLR), a β-(2-1)-fructan (DP 2 to DP 40), was studied. W57 and other bacteria grew relatively poorly on sc-inulin, with only fractions of DP 3 and DP 5 utilized, reflecting uptake via specific transport systems followed by intracellular metabolism. subsp. W20 completely used all sc-inulin components, employing an extracellular-inulinase enzyme (glycoside hydrolase family GH32 [GH32], also found in other strains of this species); the purified enzyme converted high-DP compounds into fructose, sucrose, 1-kestose, and F2 (inulobiose). The cocultivation of W57 and W20 on sc-inulin resulted in cross-feeding of the former by the latter, supported by this extracellular -inulinase. The extent of cross-feeding depended on the type of fructan, i.e., the GF type (clearly stimulating) versus the FF type (relatively low stimulus), and on fructan chain length, since relatively low-DP β-(2-1)-fructans contain a relatively high content of GF-type molecules, thus resulting in higher concentrations of GF-type DP 2 to DP 3 degradation products. The results provide an example of how cross-feeding on prebiotic β-(2-1)-fructans may occur among probiotic lactobacilli. The human gut microbial community is associated strongly with host physiology and human diseases. This observation has prompted research on pre- and probiotics, two concepts enabling specific changes in the composition of the human gut microbiome that result in beneficial effects for the host. Here, we show how fructooligosaccharide-inulin prebiotics are fermented by commercial probiotic bacterial strains involving specific sets of enzymes and transporters. Cross-feeding strains such as W20 may thus act as keystone strains in the degradation of prebiotic inulin in the human gut, and this strain--inulinase combination may be used in commercial -inulin synbiotics.

show abstract

Section: Resultsmentioning

confidence: 99%

Cross-Feeding among Probiotic Bacterial Strains on Prebiotic Inulin Involves the Extracellular exo -Inulinase of Lactobacillus paracasei Strain W20

Böger

Bueren

Dijkhuizen

2018

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Thus, it could be speculated that better biotransformation results were obtained due to good strain growth and production of more extracellular enzymes, owing to utilization of multiple carbon resources and tolerance to lactic acid. The utilization of various carbon sources and the multiple enzymes system promoted the process of biotransformation via L. paracasei [26]. In L. salivarius, the characteristic of utilizing fructo-oligosaccharides and glucose during metabolism coupled with lactic-acid tolerance could be considered as propitious for the process of biotransformation [27].…”

Section: Phylogenetic Analysis By 16s Rdna Analysismentioning

confidence: 99%

Screening and identification of probiotic Lactobacillus for the production of diosgenin from Dioscorea zingiberensis Wright by biotransformation

Shu

Wang

Chen

2017

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

The commonly used methods to produce diosgenin by acid hydrolysis generate large amounts of wastewater and other pollution. Exploring a new environmentally friendly method is becoming urgent. Mild conditions and less pollution are the advantages of biotransformation. Therefore, aiming at exploring the effect of probiotic Lactobacillus on the production of diosgenin via biotransformation, batch studies were performed. Twenty-four strains maintained in laboratory were screened by conversion rate and pH. Based on screening, purification and 16 s rDNA sequences, four probiotic strains were identified and classified. The results indicated that all the strains had biotransformation potential. Among all the strains, four strains, including L20, L35, L83 and L33, performed better, with conversion rates reaching up to 15.12%, 15.83%, 17.07% and 17.47%, respectively. The highest conversion rate, 17.47%, was that of L33 and the lowest one, 7.07%, of L4. Nineteen of the 24 strains achieved more than 10% conversion rate. The pH decreased from 7 to 3-4 after fermentation, reflecting the progress of fermentation and indicating the end of the biotransformation process. Molecular characterization based on 16S rDNA homology of partial sequences confirmed the preliminary identification as L. rhamnosus (L20, L35), L. paracasei (L83) and L. salivarius (L33).

show abstract

“…The two main uses of inulinases are the processing of high fructose syrup and fructooligosaccharides [3]. Other inulinase applications are production of bioethanol [4], lactic acid [5], single-cell oil [6], and citric acid [7].…”

Section: Introductionmentioning

confidence: 99%

Response surface methodology to optimize inulinase production by a newly isolated Penicillium amphipolaria strain by solid-state fermentation of Saccharum arundinaceum

Das¹,

Bhat²,

Selvaraj³

2020

Preprint

View full text Add to dashboard Cite

In the present investigation, a new fungal inulinase producer Penicillium amphipolaria KAS 2555 has been isolated from the soil of dead mangroves litter area, followed by the inulinase production and optimization by solid-state fermentation using a low-cost substrate – hardy sugarcane (Saccharum arundinaceum). While screening, only Penicillium amphipolaria KAS 2555 showed the hydrolysis zone on the plate containing inulin media. The exoinulolytic nature of inulinase and its form of action was confirmed by thin-layer chromatography (TLC). After 96 h of the fermentation period, an activity of 2.45 U/gds was obtained. The I/S ratio of 0.59 proved that the enzyme is inulolytic in nature. Media optimization was performed to obtain a regression model using Central Composite Design (CCD). For optimization, five significant media components viz., inulin, (NH4)2SO4, K2HPO4, KH2PO4 and, NaCl were used. A 3.10-fold increase in activity of inulinase (7.59 U/gds) was obtained under the optimal settings of (g/gds) inulin- 0.1, (NH4)2SO4- 0.002, K2HPO4- 0.1, KH2PO4- 0.02 and NaCl- 0.02.

show abstract

Direct conversion of chicory flour into l(+)-lactic acid by the highly effective inulinase producer Lactobacillus paracasei DSM 23505

Cited by 36 publications

References 17 publications

Cross-Feeding among Probiotic Bacterial Strains on Prebiotic Inulin Involves the Extracellular exo -Inulinase of Lactobacillus paracasei Strain W20

Cross-Feeding among Probiotic Bacterial Strains on Prebiotic Inulin Involves the Extracellular exo -Inulinase of Lactobacillus paracasei Strain W20

Screening and identification of probiotic Lactobacillus for the production of diosgenin from Dioscorea zingiberensis Wright by biotransformation

Response surface methodology to optimize inulinase production by a newly isolated Penicillium amphipolaria strain by solid-state fermentation of Saccharum arundinaceum

Contact Info

Product

Resources

About