Direct conversion of cellulosic material to ethanol by the intergeneric fusant Trichoderma reesei QM 9414/Saccharomyces cerevisiae NCIM 3288

Srinivas, D.; Rao, K. Jagannadha; Théodore, K.; Panda, T.

doi:10.1016/0141-0229(94)00093-7

Cited by 11 publications

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“…Pyrolysis Temperature (°C) enzymatic bioconversion of biomass into ethanol (Wayman et al 1992;Srinivas et al 1995;Oh et al 1996;Rocha et al 2013), references for ethanol, as a direct cellulose pyrolysis product, were not found. In a recent study (Jalbert et al 2015) on the ageing of paper/oil systems at temperatures between 120 and 210°C, ethanol is present as a degradation product; however, the ratio of methanol/ethanol concentrations is always higher than unity.…”

Section: Identification Of Pyrolysis Productsmentioning

confidence: 99%

Understanding ethanol versus methanol formation from insulating paper in power transformers

et al. 2015

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The life of an electrical transformer is mainly determined by that of its cellulosic solid insulation. The analysis of the chemical markers of cellulose degradation dissolved in oil is a simple and economical way to indirectly characterize the insulating paper. Methanol, a marker that is intimately linked to the rupturing of 1,4-b-glycosidic bonds of cellulose, has been observed together with ethanol during laboratory ageing experiments. Regardless of the simulated ageing conditions (temperature, humidity, air), the ratio of methanol to ethanol concentration is always higher than one (unity). However, in approximately 10 % of transformer oil samples, the ethanol generation is higher than that of methanol. In this study, thermal degradation by pyrolysis is coupled with gas chromatography/mass spectrometry to assess the volatile by-products generated at high temperatures with emphasis on methanol/ethanol generation. Some cellulose model compounds were also pyrolyzed and thermally aged in oil. The results showed that the generation of ethanol from paper pyrolysis is always smaller than for methanol, but it only occurs at temperatures higher than 300°C. However, thermal ageing of levoglucosan in oil generates a massive amount of ethanol compared to methanol regardless of the conditions (temperature, humidity, air, nitrogen, acidity). The hypothesis that ethanol is a by-product of cellulose degradation through levoglucosan as an intermediary in power transformers is proposed. The presence of ethanol during transformer oil analysis is of high interest because it can be related to a thermal fault or hot spot within the solid insulation.

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Section: Identification Of Pyrolysis Productsmentioning

confidence: 99%

Understanding ethanol versus methanol formation from insulating paper in power transformers

et al. 2015

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“…cerevisiae fusants Endoglucanase activities of M14 and M62 fusants of T. reesei/S. cerevisiae were compared using dried grass and ®lter paper cellulose (each 10 kg/m 3 ) as the cellulosic source in the medium optimized for ethanol production [6]. Glucose at a concentration of 10 kg/m 3 was taken as the control.…”

Section: 3mentioning

confidence: 99%

“…The resultant fusant has been found to be stable and produce ethanol from cellulosic substances [6]. Production of endoglucanase, one of the enzymes responsible for the conversion of cellulosic materials to fermentable sugars, is a key factor in this case.…”

Section: Introductionmentioning

confidence: 99%

“…So far only multistage approach of ethanol production has been taken up, which has its own limitations of being capital intensive, time consuming and low yielding [4]. Hence, in order to achieve a single stage conversion of cellulose to ethanol, Trichoderma reesei QM 9414, a hyper-cellulase producer, was fused with Saccharomyces cerevisiae NCIM 3288, a potent ethanol producer, in the authors' laboratory [5].The resultant fusant has been found to be stable and produce ethanol from cellulosic substances [6]. Production of endoglucanase, one of the enzymes responsible for the conversion of cellulosic materials to fermentable sugars, is a key factor in this case.…”

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confidence: 99%

“…Composition of unoptimized and optimized medium for endoglucanase production by Trichoderma reesei QM 9414 and Trichoderma reesei/Saccharomyces cerevisiae fusant Component Unoptimized (kg/m 3 ) Optimized T. reesei (WT) (kg/m 3 ) Fusant a (kg/m 3 )Medium optimized for ethanol production[6] by T. reesei/S. cerevisiae fusantTable 9.…”

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Comparative analysis of optimal endoglucanase production by Trichoderma reesei and intergeneric fusants of Trichoderma reesei Saccharomyces cerevisiae

Sinha

Panda

1998

Bioprocess Engineering

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Intergeneric fusants of Trichoderma reesei QM 9414/Saccharomyces cerevisiae NCIM 3288 developed in the authors' laboratory can convert cellulosic materials directly to ethanol in a single step process. The production of endoglucanase in this case is a key factor. The production pro®le of this enzyme by the intergeneric fusants is different from Trichoderma reesei QM 9414 (WT). The production of endoglucanase was studied seperately by Trichoderma reesei (WT) using optimal production medium which was designed as per the combined screening approach of Plackett-Burman followed by a central composite experimental plan and the intergeneric fusants using optimal production medium obtained by Box-Behnken optimization procedure. Dried grass was used as the cellulosic substance whose concentration was kept constant during the statistical optimization procedure. The concentration of dried grass was later varied keeping the other optimized medium constituents constant to ®nd the ®nal optimum medium composition for endoglucanase production. List of symbolsvalue of x i at the centre point of the investigated area Dx i step change IntroductionWith the reserves of non-replenishable energy sources slowly getting exhausted, efforts are on for tapping energy from the replenishable ones. Since ethanol can be obtained from renewable resources, it provides a promising alternative fuel source. Besides, ethanol is also used as a solvent, a germicide, an antifreeze and a chemical intermediate [1]. Ethanol is produced by fermentation by the S. cerevisiae-molasses technology. However, scanty and uneven supply of molasses and its higher consumption for more costly chemical and biochemical production renders severe handicap to this technology [2,3]. Ethanol is also produced from cellulose, an abundant biomass. So far only multistage approach of ethanol production has been taken up, which has its own limitations of being capital intensive, time consuming and low yielding [4]. Hence, in order to achieve a single stage conversion of cellulose to ethanol, Trichoderma reesei QM 9414, a hyper-cellulase producer, was fused with Saccharomyces cerevisiae NCIM 3288, a potent ethanol producer, in the authors' laboratory [5].The resultant fusant has been found to be stable and produce ethanol from cellulosic substances [6]. Production of endoglucanase, one of the enzymes responsible for the conversion of cellulosic materials to fermentable sugars, is a key factor in this case. Comparative analysis of endoglucanase production by the intergeneric fusants of T. reesei/S. cerevisiae and T. reesei (WT) under optimal production conditions using response-surface methodology has been presented. 2 Materials and methods 2.1 Microorganisms Trichoderma reesei QM 9414 was obtained from the National Chemical Laboratory, Pune, India. The intergeneric fusants of Trichoderma reesei QM 9414/Saccharomyces cerevisiae NCIM 3288 was developed in the authors' laboratory [5]. 2.2 Culture maintenance T. reesei QM 9414 was maintained on malt-agar slants, having (kg/m 3 ): malt-e...

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Liquid State Bioconversion of Domestic Wastewater Sludge for Bioethanol Production

2007

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Direct conversion of cellulosic material to ethanol by the intergeneric fusant Trichoderma reesei QM 9414/Saccharomyces cerevisiae NCIM 3288

Cited by 11 publications

References 11 publications

Understanding ethanol versus methanol formation from insulating paper in power transformers

Understanding ethanol versus methanol formation from insulating paper in power transformers

Comparative analysis of optimal endoglucanase production by Trichoderma reesei and intergeneric fusants of Trichoderma reesei Saccharomyces cerevisiae

Liquid State Bioconversion of Domestic Wastewater Sludge for Bioethanol Production

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