Direct colorimetric diagnosis of pathogen infections by utilizing thiol-labeled PCR primers and unmodified gold nanoparticles

Jung, Ye Lim; Jung, Cheulhee; Parab, Harshala; Li, Taihua; Park, Hyun Gyu

doi:10.1016/j.bios.2010.01.010

Cited by 80 publications

(31 citation statements)

References 34 publications

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“…The stabilized AuNPs would not have any color transition whereas these mixed with ds DNA underwent fast aggregation, resulting in a visual readout. Many other groups reported similar results with tailored design for detecting DNA with different sequences [60][61][62].…”

Section: Detection Of Dnasupporting

confidence: 71%

Utilization of unmodified gold nanoparticles in colorimetric detection

Liu

Wang

et al. 2011

Sci. China Phys. Mech. Astron.

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This review begins with an overview of the appealing properties and various applications of gold nanoparticles, and briefly summarizes recent advances in using unmodified gold nanoparticles to detect different kinds of targets including nucleic acids, proteins, metal ions and small organic molecules. The key point to the unmodified gold nanoparticle-based visual detection assay is to control dispersion and aggregation of colloidal nanoparticles by targets of interest, which usually relies on affinities between gold nanoparticles and targets. The degree of dispersion or aggregation can be visualized through the change of the solution color or the precipitation of nanoparticles from the solution. Thus, the existence of the target molecules can be translated into optical signals and monitored by the naked eye conveniently. Finally, some future prospects of this research field are given. gold nanoparticles, colorimetric detection, biosensor

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Section: Detection Of Dnasupporting

confidence: 71%

Utilization of unmodified gold nanoparticles in colorimetric detection

Liu

Wang

et al. 2011

Sci. China Phys. Mech. Astron.

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“…Nevertheless, culture has a long turnaround time, and RT-PCR requires expensive instruments and infrastructure. In contrast, UV-vis spectroscopy is usually available in laboratories and can further confirm the visually identified results of the nanoparticles (Jung, Jung, Parab, Li, & Park, 2010). It should be noted also, that the platform can accommodate detection of many bacterial species simultaneously, allowing multiplexing, high throughput screening and expedite turnaround time of clinical, veterinary and food specimens.…”

Section: Resultsmentioning

confidence: 99%

A Simple Gold Nanoprobe Assay for the Identification of Staphylococcus Aureus, Listeria Monocytogenes and Salmonella Enteritidis in Food Specimens

Houhoula¹,

Charvalos²,

Konteles³

et al. 2017

JFR

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In the present study, we developed a gold nanoprobe assay, which relies on the colorimetric differentiation of specific DNA sequences, based on different aggregation profiles. We evaluated the assay on DNA extracted from pathogen cultures and from contaminated food specimens. The targets used were the femA gene for the identification of Staphylococcus aureus, the hly gene of the Listeria monocytogenes listeriolysin and the invA sequence for Salmonella spp. Comparison was performed with the reference assay, as described in the respective ISO guidelines for each pathogen, and a direct PCR amplification and detection. The minimum detection limit of the assay was defined at 123 fg/μL DNA, for all species, lower than PCR detection. Specificity was 100%. Sensitivity was 100%, as compared το the reference method, whereas the sensitivity of PCR was 93.3%. The evaluated assay could be used as a sensitive screening method, which does not require major infrastructure, for the detection and identification of pathogens in food specimens. In addition, it can accommodate detection of multiple species, thus allowing multiplexing and expedite turnaround time.

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“…Metal NP-based homogeneous colorimetric detection of oligonucleotides holds great promise due to the low cost, low volume, and rapid readout of a target DNA [88]. A homogeneous colorimetric DNA biosensor has been developed by a novel nicking endonuclease-assisted NP amplification process that is capable of recognizing long single-stranded oligonucleotides with single-base mismatch selectivity and a 10 3 -fold improvement in amplification (ca.…”

Section: Colorimetric Detection Of Bioanalytesmentioning

confidence: 99%