Biofunctionalization of Nanomaterials

Ju, Huangxian; Zhang, Xueji; Wang, Joseph

doi:10.1007/978-1-4419-9622-0_1

Cited by 5 publications

(6 citation statements)

References 135 publications

(144 reference statements)

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“…Spheroid culturing of the magnetic labeled cells was performed by our predefined microwell design in which a group of spheroids with the same size and geometry can be acquired in 5 days . In this method, a 3D printing-based microwell made of agarose hydrogel was soaked in growth media in a 12-well plate and magnetic captured cells were dispensed in 500 μL of media and loaded on the microwell.…”

Section: Methodsmentioning

confidence: 99%

“…Molecular probes used in immunomagnetic CTC isolation approaches include antibodies and aptamers that are engineered to detect specific surface proteins on CTCs. , Among biomoieties, antibodies are the promising ones for targeting cancer cells, using high affinity and ligand–receptor specificity for the surface antigen on the tumor. , Antibodies can be conjugated to magnetic particles by absorption or using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride– N -hydroxysuccinimide (EDC–NHS) chemistry. However, the random orientation of antibodies on the surface of the particles reduces their capturing efficiency.…”

Section: Introductionmentioning

confidence: 99%

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Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells

Seyfoori

Ebrahimi

Samiei

et al. 2019

ACS Appl. Mater. Interfaces

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Circulating tumor cells are of utmost importance among various biomarkers in liquid biopsies as a prognosis indicator of metastasis as well as in chemotherapeutic monitoring. This study introduces an efficient tool composed of soft nano/hybrid immune microgels for magnetic isolation of targeted tumor cells. The development process involves the in situ synthesis of magnetic nanoparticles within the three-dimensional matrix of thermoresponsive microgels. Surface modification and anti-EpCAM conjugation are adjusted by changing the temperature, and a conjugation efficiency of around 70% is achieved by using a protein G linker. Anti-EpCAM-conjugated nano/hybrid magnetic microgels are used to isolate EpCAM-expressing breast adenocarcinoma MCF-7 cells from culture media and whole blood with an efficiency of 75 and 70%, respectively. Furthermore, we demonstrate the ability of the hybrid microgels to isolate cancer cells with a purity of 65% and culture the cells post-isolation for further drug studies. The multifunctional hybrid microcarriers reported in this work can be potentially used for continuous monitoring of cancers and in personalized medicine.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells

Seyfoori

Ebrahimi

Samiei

et al. 2019

ACS Appl. Mater. Interfaces

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“…Hydrophilic and hydrophobic surfaces can affect the interaction of nanomaterials with biomolecules and proteins adsorption. Related nanomaterials and nanostructures surface modification reviews [ 40 , 41 , 42 , 43 , 44 , 45 ] have demonstrated various chemical and physical modifications and bioconjugation methods. Various functional groups and applications of nanomaterials and nanostructures can be visualized with suitable surface modification and fabrications.…”

Section: Fluorescence-emitting/interacting Nanomaterialsmentioning

confidence: 99%

Fluorescent Nanobiosensors for Sensing Glucose

Chen

Hwang

Zhang

2018

Sensors

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Glucose sensing in diabetes diagnosis and therapy is of great importance due to the prevalence of diabetes in the world. Furthermore, glucose sensing is also critical in the food and drug industries. Sensing glucose has been accomplished through various strategies, such as electrochemical or optical methods. Novel transducers made with nanomaterials that integrate fluorescent techniques have allowed for the development of advanced glucose sensors with superior sensitivity and convenience. In this review, glucose sensing by fluorescent nanobiosensor systems is discussed. Firstly, typical fluorescence emitting/interacting nanomaterials utilized in various glucose assays are discussed. Secondly, strategies for integrating fluorescent nanomaterials and biological sensing elements are reviewed and discussed. In summary, this review highlights the applicability of fluorescent nanomaterials, which makes them ideal for glucose sensing. Insight on the future direction of fluorescent nanobiosensor systems is also provided.

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“…Biofunctionalization via non-covalent assembly can be categorized into physical adsorption and affinity interactions (Ju et al, 2011). Many of the coating polymers and chemistries result in a charged surface on the particles, an effect which can be then harnessed for direct adsorption of the biological agent of interest.…”

Section: Magnetophoretic Cell Separation: Theory and Phenomenamentioning

confidence: 99%

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

2014

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Magnetic sorting using magnetic beads has become a routine methodology for the separation of key cell populations from biological suspensions. Due to the inherent ability of magnets to provide forces at a distance, magnetic cell manipulation is now a standardized process step in numerous processes in tissue engineering, medicine, and in fundamental biological research. Herein we review the current status of magnetic particles to enable isolation and separation of cells, with a strong focus on the fundamental governing physical phenomena, properties and syntheses of magnetic particles and on current applications of magnet-based cell separation in laboratory and clinical settings. We highlight the contribution of cell separation to biomedical research and medicine and detail modern cell separation methods (both magnetic and non-magnetic). In addition to a review of the current state-of-the-art in magnet-based cell sorting, we discuss current challenges and available opportunities for further research, development and commercialization of magnetic particle-based cell separation systems.

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Biofunctionalization of Nanomaterials

Cited by 5 publications

References 135 publications

Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells

Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells

Fluorescent Nanobiosensors for Sensing Glucose

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

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