Direct and sensitive detection of a human virus by rupture event scanning

Cooper, Matthew A.; Dultsev, F. N.; Minson, Tony; Ostanin, Victor P.; Abell, Chris; Klenerman, David

doi:10.1038/nbt0901-833

Cited by 168 publications

(126 citation statements)

References 14 publications

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“…In contrast, no significant signal change is observed for electrodes incubated in DNA-free hybridization buffer or in the presence of the highest noncognate DNA concentrations we have investigated (10 M oligo 3). Thus, the selectivity of the sensor is in excess of 10 6 . The E-DNA sensor also exhibits exceptional dynamic range; peak currents are logarithmically related to target concentration across the almost 6-decade range we have investigated (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Consequently, a variety of optical (2)(3)(4)(5), acoustic (6,7), and electronic (8,9) ''gene detection'' approaches has been reported. Among these, a solutionphase optical approach termed molecular beacons has attracted significant interest (10) because of its applicability to approaches ranging from in vitro genotyping (11) to in vivo studies within single cells (12).…”

mentioning

confidence: 99%

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Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA

Fan

Plaxco²,

Heeger³

2003

Proc. Natl. Acad. Sci. U.S.A.

1,004

896

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We report a strategy for the reagentless transduction of DNA hybridization into a readily detectable electrochemical signal by means of a conformational change analogous to the optical molecular beacon approach. The strategy involves an electroactive, ferrocene-tagged DNA stem-loop structure that self-assembles onto a gold electrode by means of facile gold-thiol chemistry. Hybridization induces a large conformational change in this surface-confined DNA structure, which in turn significantly alters the electron-transfer tunneling distance between the electrode and the redoxable label. The resulting change in electron transfer efficiency is readily measured by cyclic voltammetry at target DNA concentrations as low as 10 pM. In contrast to existing optical approaches, an electrochemical DNA (E-DNA) sensor built on this strategy can detect femtomoles of target DNA without employing cumbersome and expensive optics, light sources, or photodetectors. In contrast to previously reported electrochemical approaches, the E-DNA sensor achieves this impressive sensitivity without the use of exogenous reagents and without sacrificing selectivity or reusability. The E-DNA sensor thus offers the promise of convenient, reusable detection of picomolar DNA.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA

Fan

Plaxco²,

Heeger³

2003

Proc. Natl. Acad. Sci. U.S.A.

1,004

896

View full text Add to dashboard Cite

show abstract

“…1,2 To date, a variety optical, [3][4][5][6] electronic, 7 acoustic, 8 and gravimetric 9,10 techniques have been applied to this goal. Among these, the electrochemical detection of DNA hybridization appears promising due to its rapid response time, low cost, and suitability for mass production.…”

Section: Introductionmentioning

confidence: 99%

Effect of Molecular Crowding on the Response of an Electrochemical DNA Sensor

et al. 2007

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E-DNA sensors, the electrochemical equivalent of molecular beacons, appear to be a promising means of detecting oligonucleotides. E-DNA sensors are comprised of a redox-modified (here, methylene blue or ferrocene) DNA stem-loop covalently attached to an interrogating electrode. Because E-DNA signaling arises due to binding-induced changes in the conformation of the stemloop probe, it is likely sensitive to the nature of the molecular packing on the electrode surface. Here we detail the effects of probe density, target length, and other aspects of molecular crowding on the signaling properties, specificity, and response time of a model E-DNA sensor. We find that the highest signal suppression is obtained at the highest probe densities investigated, and that greater suppression is observed with longer and bulkier targets. In contrast, sensor equilibration time slows monotonically with increasing probe density, and the specificity of hybridization is not significantly affected. In addition to providing insight into the optimization of electrochemical DNA sensors, these results suggest that E-DNA signaling arises due to hybridization-linked changes in the rate, and thus efficiency, with which the redox moiety collides with the electrode and transfers electrons.

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“…8. A specific feature of this signal in comparison, for example, with the rupture signal of herpes virus [5] is that HBsAg rupture signal has not a narrow peak but a broad peak. This shape suggests that rupture occurs within a broad voltage range.…”

Section: Resultsmentioning

confidence: 99%

“…It turned out to be possible to broaden the measuring possibilities and to enhance the sensitivity of QCM-based sensors by applying the principle of the measurement of the acoustic signal generated during the rupture of bonds holding an object on the QCM surface, as proposed in [4]. The applications of this method to the determination of phages and bacteria were demonstrated in [5,6].…”

Section: Introductionmentioning

confidence: 99%

Shape of the Voltage–Frequency Curve Depending on the Type of the Object Detached from the QCM Surface

Dultsev

2017

Proceedings of the Scientific-Practical Conference "Research and Development - 2016"

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Analysis of the shapes of voltage-frequency curves depending on the type of object detached from the surface of the quartz crystal microbalance (QCM) is carried out. It is demonstrated that the shape of the curve depends not only on the size and shape of bio-object but also on the properties of the particle. For example, a detachment of hepatitis B virus is accompanied by the fragmentation of the bio-object, and signal shape is typical for this case. In addition to a voltage value which determines the bonding force, the signal shape is also characteristic for identification of bio-object.

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Direct and sensitive detection of a human virus by rupture event scanning

Cited by 168 publications

References 14 publications

Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA

Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA

Effect of Molecular Crowding on the Response of an Electrochemical DNA Sensor

Shape of the Voltage–Frequency Curve Depending on the Type of the Object Detached from the QCM Surface

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