2007
DOI: 10.1021/la700328r
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Effect of Molecular Crowding on the Response of an Electrochemical DNA Sensor

Abstract: E-DNA sensors, the electrochemical equivalent of molecular beacons, appear to be a promising means of detecting oligonucleotides. E-DNA sensors are comprised of a redox-modified (here, methylene blue or ferrocene) DNA stem-loop covalently attached to an interrogating electrode. Because E-DNA signaling arises due to binding-induced changes in the conformation of the stemloop probe, it is likely sensitive to the nature of the molecular packing on the electrode surface. Here we detail the effects of probe density… Show more

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Cited by 299 publications
(393 citation statements)
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“…We did so by assuming that the density ratios on surface are linearly correlated with the concentration ratios deployed in solution during deposition. This (seemingly reasonable) assumption seems confirmed by the linear dependence of the absolute current signals (which are correlated to surface density [14] Figure SI1). However, we note that this correlation could be more complicated for less defined recognition elements which can induce a non-linear immobilization of probe and depletant ( Figure SI2).…”
supporting
confidence: 58%
“…We did so by assuming that the density ratios on surface are linearly correlated with the concentration ratios deployed in solution during deposition. This (seemingly reasonable) assumption seems confirmed by the linear dependence of the absolute current signals (which are correlated to surface density [14] Figure SI1). However, we note that this correlation could be more complicated for less defined recognition elements which can induce a non-linear immobilization of probe and depletant ( Figure SI2).…”
supporting
confidence: 58%
“…These include applications to pharmacology, environmental pollution monitoring, and food industry. In particular, biological systems are often characterized by complex chemical pathways whose modeling is rather challenging and can not be recast in standard schemes [3][4][5][6][7][8][9][10][11][12][13][14][15] (see also [16][17][18][19] for a different perspective). In general, one tries to split such sophisticated systems into a set of elementary constituents, in mutual interaction, and for which a clear formalization is available [20][21][22][23][24][25].…”
Section: The Chemical Kineticsmentioning
confidence: 99%
“…In parallel, gold surfaces were activated using the method previously described. Then, (DTPA) 2 In4Fc functionalized electrode was prepared by soaking the freshly activated gold surface at room temperature under argon in 96µL of phosphate buffer solution (PB, 100 mM), pH8 containing reduced (DTPA) 2 In4Fc (10 µM) and TCEP/HCl (1.6 mM) during 3 days, according to the protocol described by Ricci et al [18]. Then, after rinsing the electrode with water, the system was passivated by soaking for 1 h in 60 µL of a solution of mercaptopropanol (1 mM) in water and finally rinsed with water.…”
Section: Electrode Functionalization With the Tetrathiol-modified Olimentioning
confidence: 99%