Direct and efficient xylitol production from xylan by Saccharomyces cerevisiae through transcriptional level and fermentation processing optimizations

Li, Zhe; Qu, Hongnan; Li, Chun; Zhou, Xiaohong

doi:10.1016/j.biortech.2013.09.101

Cited by 45 publications

(18 citation statements)

References 30 publications

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“…Although overexpressing UPC2‐1 in SGibS strain increased more β‐amyrin (3.8%) than that in SGib (2.5%), the positive effect was slight, demonstrating that regulating terpenoid pathway through refactored promoters with SRE is more effective than that of overexpressing UPC2‐1 (Table S6, Supporting Information). The reconstructed promoters with SRE including P ALA1 , P GPM1 , and P FBA1 were used to replace corresponding wild promoters in xylitol producing S. cerevisiae that was previously constructed by our group, and xylitol production was improved 29.8% (Supporting Information methods 1.4 and Figure S5), showing the expandability of this strategy. Therefore, the strategy using fused UPC2 binding site to regulate pathway is not exclusive to β‐amyrin, and may serve as a novel general road for balancing endogenous and exogenous pathways of engineered yeast.…”

Section: Discussionmentioning

confidence: 99%

Refactoring β‐amyrin synthesis in Saccharomyces cerevisiae

et al. 2015

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Triterpenoids are a highly diverse group of natural products and used particularly as medicine. Here, a strategy combining stepwise metabolic engineering and transcriptional control was developed to strengthen triterpenoid biosynthesis in Saccharomyces cerevisiae. Consequently, an efficient biosynthetic pathway for producing β‐amyrin, a commercially valuable compound and precursor of triterpenoids, was constructed through expressing a plant‐derived β‐amyrin synthase. Introducing a heterologous squalene monooxygenase greatly dragged intermediate metabolite squalene toward β‐amyrin. Increasing squalene pool by overexpressing IPP isomerase, FPP, and squalene synthase further enhanced β‐amyrin synthesis of 49‐folds. Through reconstructing the promoters with the binding site of transcription factor UPC2, directed transcriptional regulation on engineered pathway was availably achieved, resulting in β‐amyrin titer increased by 65‐folds. Using ethanol fed‐batch fermentation, β‐amyrin titer was finally improved up to 138.80 mg/L with a yield of 16.30 mg/g dry cell, almost 185 and 232 and folds of the initially engineered strain, respectively. © 2015 American Institute of Chemical Engineers AIChE J, 61: 3172–3179, 2015

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Section: Discussionmentioning

confidence: 99%

Refactoring β‐amyrin synthesis in Saccharomyces cerevisiae

et al. 2015

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“…Xylitol is a five-carbon polyols derived from xylose of hemicellulose and also has been selected as one of top 12 high value-added intermediate chemicals [49]. It is produced by chemical hydrogenation process via nickel catalyst [50].…”

Section: Xylitolmentioning

confidence: 99%

Renewable Hydrogen Produced from Different Renewable Feedstock by Aqueous-Phase Reforming Process

Wei¹,

Lei²,

Liu³

et al. 2014

JSBS

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Aqueous phase reforming (APR) of biomass derived feedstock producing hydrogen was reviewed. The APR process was discussed based on different feedstock categories such as sugars, polyols and ethanol. The mechanism of APR was analyzed referring to different structures of feedstock. The reaction pathways of APR were investigated. The usage of catalysts should be judged by feedstock on the requirement including C-C bond cleavage, water-gas shift (WGS) reaction, and catalyst maintenance. The prospects were concluded based on the recent works from bimetallic catalysts and high efficient supports. Examples of significant challenges of reducing catalyst cost and increasing catalyst stability have been discussed. The modification and utilization of alkane selectivity of APR processes for liquid fuel production was also investigated.

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“…A xylitol yield of 0.71 g g −1 xylan was obtained by optimizing the transcriptional regulation and fermentation processes. (81) In other research, the reduction of xylose to xylitol from hemicellulose hydrolysate was successfully carried out with engineered Escherichia coli by eliminating L-arabinitol formation to produce xylitol at almost 100% purity. (82) Downloaded by [University of Newcastle, Australia] at 10:53 05 January 2015…”

Section: Developing New Techniques For Xylitol-producing Microbesmentioning

confidence: 99%

Xylitol Biological Production: A Review of Recent Studies

Mohamad

Kamal

Mokhtar

2014

Food Reviews International

103

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Xylitol is an alternative sweetener that is recommended for use in food and pharmaceutical products, as it has some health benefits. It is currently produced on a large scale using a chemical reduction that requires high energy and is costly. Biological conversion of xylitol using microorganisms is an alternative process that is environmentally friendly and cost-effective. This process has been studied in an effort to provide one that is high yielding and competitive with chemical processes. This article reviews recent studies in the development of biological conversion processes for the production of xylitol, including biomass conversion, fermenting microorganisms, and new technology for full-scale process development.

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Direct and efficient xylitol production from xylan by Saccharomyces cerevisiae through transcriptional level and fermentation processing optimizations

Cited by 45 publications

References 30 publications

Refactoring β‐amyrin synthesis in Saccharomyces cerevisiae

Refactoring β‐amyrin synthesis in Saccharomyces cerevisiae

Renewable Hydrogen Produced from Different Renewable Feedstock by Aqueous-Phase Reforming Process

Xylitol Biological Production: A Review of Recent Studies

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