Direct and callus-mediated regeneration of Curcuma soloensis Valeton (Zingiberaceae) and ex vitro performance of regenerated plants

Zhang, Shijun; Liu, Nian; Ai-wu, Sheng; Ma, Guohua; Wu, Guojiang

doi:10.1016/j.scienta.2011.08.038

Cited by 18 publications

(9 citation statements)

References 23 publications

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“…with a mean of 8.1 shoots/explant. These studies are similar to that of Zhang et al [17], in which it was found that, when TDZ was added to the growing medium of Curcuma soloensis Valeton., it resulted in over 3 times the number of shoots as BA (18.7 or 5.0 shoots/explant from media, with 2.5 µM TDZ or 40 µM BA, respectively). Higher concentrations of TDZ had no inhibitory effects on shoot multiplication but decreased the callus formation percentage.…”

Section: Resultssupporting

confidence: 89%

In vitro Propagation of Globba schomburgkii Hook. f. via Bulbil Explants

Saensouk

Pimmuen

2017

Walailak J Sci & Tech

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An efficient and rapid protocol for the micropropagation of Globba schomburgkii Hook. f. via bulbil explants was investigated. The long divided and undivided bubils of G. schomburgkii Hook. f. were cultured on MS medium (Murashige and Skoog) that had either 3 mg/l benzyladenine (BA) or 0.5 mg/l naphthaleneacetic acid (NAA) added for 8 weeks. The results indicated that the long divided bulbils of G. schomburgkii Hook. f. showed a greater amount of plant regeneration than the undivided bulbils. Callus induction, as well as shoot and root formation, were observed when culturing microshoots of 1 cm in length on media (MS) that had Thidiazuron (TDZ) or NAA plus BA added at a range of concentrations for 8 weeks. The highest percentage of callus induction was 40 % when culturing the microshoots on MS medium supplemented with NAA and BA. The best result for shoot formation was achieved when culturing the microshoots on MS medium with TDZ added. The highest number of roots was obtained when culturing the microshoots on MS medium with NAA and BA added. The in vitro-derived plantlets of G. schomburgkii Hook. f. were transplanted to pots containing different types of potting mixture in a greenhouse. The survival rates were 80 % when G. schomburgkii Hook. f. was transplanted to sand.

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Section: Resultssupporting

confidence: 89%

In vitro Propagation of Globba schomburgkii Hook. f. via Bulbil Explants

Saensouk

Pimmuen

2017

Walailak J Sci & Tech

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“…Prathanturarug et al (2005) reported high frequency shoot induction from terminal bud explants of C. longa using TDZ. In C. soloensis TDZ containing medium induced more than three times as many shoots as BA, when vegetative buds were used as explants (Zhang et al 2011). TDZ, a substituted phenylurea, is widely used for plant growth and development studies due to its more active nature than conventionally used cytokinins (Thomas 2003).…”

Section: Resultsmentioning

confidence: 99%

Abiotic stresses increase plant regeneration ability of rhizome explants of Curcuma caesia Roxb.

Jose

Thomas

2015

Plant Cell Tiss Organ Cult

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Curcuma caesia Roxb., commonly called ''Black turmeric'', is an important medicinal plant. Normally this plant is micropropagated via rhizome or rhizome bud explant culture. However, the shoot induction from rhizome explants is low and thus, alternate methods could be adopted to induce maximum shoots. In this report, we subjected the rhizome segments to abiotic stresses such as warm water, hot air, cold water and sodium chloride (NaCl) at various time intervals for testing their effect on shoot induction. The best shoot induction medium (control) in terms of percent explants producing shoots (66 %) and number of shoots (11.2 per explant) was Murashige and Skoog medium supplemented with 6-thidiazuron (6.8 lM) and indole-3-butyric acid (1.5 lM). The application of various stresses significantly increased both percent response and number of shoots. Rhizome explants exposed to 1 or 2 h at 30-36°C warm water significantly improved the response. Optimum response (94 % cultures responding with 22.4 shoots per explants) was observed when rhizomes were exposed at 33°C for 1 h. In hot air as well as cold water treatments the percent cultures responding and number of shoots showed moderate increase. The increase brought about by 300 and 500 mM NaCl for 1-3 h was significantly higher than control. The rhizomes pretreated with 500 mM NaCl for 3 h showed highest response in contrast to other treatments. Here, 96 % rhizomes responded with an average number of 23.4 shoots per explant. These observations supported the positive role of abiotic stresses in inducing shoots from rhizome segments of C. caesia in cultures.

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“…The plant has gained wide pharmaceutical applications such as cream, lotions, capsule tablets, herbal tea, and more [1]. Tissue culture technique is widely accepted in clonal propagation for large-scale medicinal and genetically improved plants [2,3]. Tissue culture-controlled growth environment conditions provide a proper supply of nutrients, pH, temperature, gaseous and liquid environment [4].…”

Section: Introductionmentioning

confidence: 99%

Surface sterilization method for reducing contamination of Clinacanthus nutans nodal explants intended for in-vitro culture

et al. 2021

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Surface sterilization is a vital step in preparation of healthy and viable explants in tissue culture. Most surface contaminants can be eliminated by surface sterilization with a suitable sterilizing agent. The study aimed to present an effective disinfection method for Clinacanthus nutans shoot regeneration using nodal segments. A total of four different sterilization approaches were conducted by treating nodal explants with various concentrations of sterilizing agent. Sterilizing agents used were Rhizophora apiculata Pyroligneous acid (PA), sodium hypochlorite (Clorox) thiophanate-methyl (fungicide), and Mercuric chloride (HgCl2). Nodal explant then was cultured on plant growth regulator-free Murashige and Skoog (MS) basal medium. This study sterilizing agents revealed that PA showed strong bactericidal activity. However, it led to a high number of fungal contaminations. The pyroligneous acid did not exhibit a strong potential as a disinfectant for C. nutans nodal explant. Overall, HgCl2 exhibits the best reduction in fungal contamination and gives a significant result with thiophanate-methyl fungicide. Surface sterilization with mercuric chloride (0.2%) for 1 hour was the optimum concentration and duration, which resulted in the highest percentage of nodal explant survival and viability. All viable nodal segments developed into shoots. It had been concluded that the best surface sterilization agent was HgCl2.

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Direct and callus-mediated regeneration of Curcuma soloensis Valeton (Zingiberaceae) and ex vitro performance of regenerated plants

Cited by 18 publications

References 23 publications

In vitro Propagation of Globba schomburgkii Hook. f. via Bulbil Explants

In vitro Propagation of Globba schomburgkii Hook. f. via Bulbil Explants

Abiotic stresses increase plant regeneration ability of rhizome explants of Curcuma caesia Roxb.

Surface sterilization method for reducing contamination of Clinacanthus nutans nodal explants intended for in-vitro culture

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