In vitro Propagation of Globba schomburgkii Hook. f. via Bulbil Explants

Abstract

An efficient and rapid protocol for the micropropagation of Globba schomburgkii Hook. f. via bulbil explants was investigated. The long divided and undivided bubils of G. schomburgkii Hook. f. were cultured on MS medium (Murashige and Skoog) that had either 3 mg/l benzyladenine (BA) or 0.5 mg/l naphthaleneacetic acid (NAA) added for 8 weeks. The results indicated that the long divided bulbils of G. schomburgkii Hook. f. showed a greater amount of plant regeneration than the undivided bulbils. Callus induction, as well as shoot and root formation, were observed when culturing microshoots of 1 cm in length on media (MS) that had Thidiazuron (TDZ) or NAA plus BA added at a range of concentrations for 8 weeks. The highest percentage of callus induction was 40 % when culturing the microshoots on MS medium supplemented with NAA and BA. The best result for shoot formation was achieved when culturing the microshoots on MS medium with TDZ added. The highest number of roots was obtained when culturing the microshoots on MS medium with NAA and BA added. The in vitro-derived plantlets of G. schomburgkii Hook. f. were transplanted to pots containing different types of potting mixture in a greenhouse. The survival rates were 80 % when G. schomburgkii Hook. f. was transplanted to sand.

In vitro Propagation and Determination of Total Phenolic Compounds, Flavonoid Contents and Antioxidative Activity of Globba globulifera Gagnep

“…The plantlet was healthy and morphologically like the mother plants after being transferred to the field. These results differ from those of Saensouk et al 5 who reported that when the plantlets of G. schomburgkii were transferred to pots containing sand they showed the highest percentage of surviving plantlets (80%), average number of leaves per shoot (21.62) and average shoot length (15.00 cm). In this study, in vitro plantlets derived from tissue culture techniques produced healthy and plants morphologically similar to the mother after being transferred to the field.…”

“…These results are different from Parida et al 12 who reported the maximum number of shoots was 5.8 shoots/explant when culturing G. marantina on a medium that contained 3 mg/l Kinetin. The result of comparing different plant growth regulators on liquid media showed the differences from Saensouk et al 5 who reported in vitro propagation of G. schomburgkii on MS medium supplemented with 2.00 mg/l of TDZ (9.10 shoots/explant).…”

“…Nevertheless, these results differed from those of Chuengpanya et al 4 who reported that the maximum number of shoots was 24.1 shoots/explant and 180.4 roots/explant when culturing G. siamensis on MS medium containing 2.0 mg/l of BAP. Saensouk et al 5 reported different results, the MS medium supplemented with 3.0 mg/l of BAP show the number of shoots was 3.80 shoots/explant of G. schomberkii. Then, 5 mg/l of Kinetin showed the best response on shoot and root induction.…”

“…The plant growth regulators were BAP (0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/l), Kinetin (0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/l), BAP (1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) in combination with NAA (0.5 and 1 mg/l), BAP (1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) in combination with IBA (0.5 and 1 mg/l), Kinetin (1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) in combination with NAA (0.5 and 1 mg/l) and Kinetin (1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) in combination with IBA (0.5 and 1 mg/l). The MS liquid medium supplemented with 3.0 mg/l of Kinetin in combination with 0.5 mg/l of NAA (Parida et al) 3 , 2.0 mg/l of BAP (Chuengpanya et al) 4 and 2.0 mg/l of TDZ (Saensouk et al) 5 was used for the culture media. The explants were cultured on liquid and solid (7 g/l of bacto agar) MS media to study the effect of the type of media.…”