Direct Analysis in Real Time Coupled with Dried Spot Sampling for Bioanalysis in a Drug-Discovery Setting

Crawford, Elizabeth A.; Gordon, Justin; Wu, Jingtao; Musselman, Brian; Liu, Ray; Yu, Sheng

doi:10.4155/bio.11.99

Cited by 50 publications

(38 citation statements)

References 7 publications

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“…The possibility of analysis of dried spots of liquids on a carrier using DART-MS, reported only in two studies [22,23] for spots of milk on paper and for DBS on glass slides, has been confirmed using an HPTLC plate as a porous carrier and the DPS scanning analysis by the DART ion source with an adjustable angle. This gives rise for further studies of dried spots on a flat carrier by DART-MS-used for the confirmation of liquid sample analysis or as an independent analytical method.…”

Section: Discussionmentioning

confidence: 99%

“…The thirdgeneration DART ion source "DART-SVPA" is capable of being set off axis to the sampling surface for direct surface analyses, giving rise to the studies in scanning surface analysis using DART-MS. Using such a source, the capability of quantitation of several drugs from DBS on glass slides has been proven using a triple quadrupole mass spectrometer in a pharmacokinetic study [23]. To our knowledge, no other studies on DART-MS analysis of dried spots on a carrier have been performed until now.…”

Section: Analysis Of Dried Propolis Spots (Dps)mentioning

confidence: 98%

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DART-Orbitrap MS: a novel mass spectrometric approach for the identification of phenolic compounds in propolis

et al. 2012

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This is the first direct analysis in real-time mass spectrometry (DART-MS) study of propolis and a first study on the analysis of bee products using high-resolution DART-MS (DART-HRMS). Identification of flavonoids and other phenolic compounds in propolis using direct analysis in real-time coupling with Orbitrap mass spectrometry (DART-Orbitrap MS) was performed in the negative ion mode for minimizing the matrix effects, while the positive ion mode was used for the confirmation of selected compounds. Possible elemental formulae were suggested for marker components. The duration of one sample analysis by DART-MS analysis lasted ca. 30 s, and all benefits of high-resolution mass spectrometry were used upon data processing using the coupling of DART with the Orbitrap mass spectrometer. The possibility for scanning analysis of dried propolis extract spots on a planar porous surface was investigated in the heated gas flow of the DART ion source with adjustable angle. As an independent method, the approach of scanning analysis is of high interest and of future potential for confirmation of the results obtained from liquid sample analysis. Scanning analysis is highly promising for further development in the bioanalytical field due to the convenience of the storage and transportation of dried sample spots.

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Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Dried Propolis Spots (Dps)mentioning

confidence: 98%

DART-Orbitrap MS: a novel mass spectrometric approach for the identification of phenolic compounds in propolis

et al. 2012

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“…These constraints are greatly relieved by 'microsampling', resulting in the generation of DBS and allowing serial sampling from a strongly reduced amount of laboratory animals, including small rodents such as mice. This allows the replacement of composite pharmaco-or toxicokinetic profiles (obtained from different animals) by serial profiles (obtained within individual animals), which leads to higher data quality ( Clark et al, 2010, Turpin et al, 2010, Crawford et al, 2011. Apart from a large improvement in animal welfare, DBS sampling is also beneficial for the (pharmaceutical) companies involved.…”

Section: Analysis Of Therapeutic Drugs -Toxicokineticsmentioning

confidence: 99%

“…Examples include the automated analysis of DBS, the on-line extraction and analysis of DBS ('direct elution') and direct desorption of DBS (e.g. Crawford et al, 2011, Déglon et al, 2011a. These new developments have recently been reviewed elsewhere (Déglon et al, 2011b, Abu-Rabie, 2011.…”

Section: Analysis Of Therapeutic Drugs -Toxicokineticsmentioning

confidence: 99%

Dried blood spots in toxicology: from the cradle to the grave?

Stove

Ingels

Kesel

et al. 2012

Critical Reviews in Toxicology

145

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About a century after its first described application by Ivar Bang, the potential of sampling via dried blood spots (DBS) as an alternative for classical venous blood sampling is increasingly recognized. Perhaps best known is the use of DBS in newborn screening programs, ignited by the hallmark paper by Guthrie and Susi half a century ago. However, it is only recently that both academia and industry have recognized the many advantages that DBS sampling may offer for bioanalytical purposes, as reflected by the strong increase in published reports during the last few years. Currently, major DBS applications include newborn screening for metabolic disorders, epidemiological surveys (e.g. HIV monitoring), therapeutic drug monitoring (TDM), as well as toxicology. In this review, we provide a comprehensive overview of the distinct subdisciplines of toxicology for which DBS sampling has been applied. DBS sampling for toxicological evaluation has been performed from birth until autopsy, aiming at the assessment of therapeutic drugs, drugs of abuse, environmental contaminants, toxins, as well as (trace) elements, with applications situated in fields as toxicokinetics, epidemiology and environmental and forensic toxicology. We discuss the strengths and limitations of DBS in the different subdisciplines and provide future prospects for the use of this promising sampling technique in toxicology.

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“…[49,74] Another use of DART is the analysis of, covalently bound, organic monolayers on different substrates. [75,76] The application of DART in the field of forensics is not just limited to the detection of (pharmaceutical) drugs from solid samples, [77] plant material, [78] and cotton-swab wipes, [79] DART has also be used to analyze urine samples, [80] dried blood spots, [81] and explosives. [82] Aim of this research…”

Section: Applications Of Dartmentioning

confidence: 99%

Improved forensic hair evidence for drugs of abuse by mass spectrometry

Duvivier

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Direct Analysis in Real Time Coupled with Dried Spot Sampling for Bioanalysis in a Drug-Discovery Setting

Abstract: The DART-MS/MS method was applied to DBS sampling for PK/TK studies and also for in vitro screening of absorption, distribution, metabolism and excretion properties. The results from the DART-MS/MS approach correlated well with the LC-MS/MS analyses for comparison.

Cited by 50 publications

References 7 publications

DART-Orbitrap MS: a novel mass spectrometric approach for the identification of phenolic compounds in propolis

DART-Orbitrap MS: a novel mass spectrometric approach for the identification of phenolic compounds in propolis

Dried blood spots in toxicology: from the cradle to the grave?

Improved forensic hair evidence for drugs of abuse by mass spectrometry

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