DART-Orbitrap MS: a novel mass spectrometric approach for the identification of phenolic compounds in propolis

Chernetsova, Elena S.; Bromirski, Maciej; Scheibner, Olaf; Morlock, Gertrud E.

doi:10.1007/s00216-012-5800-6

Cited by 49 publications

(21 citation statements)

References 19 publications

(30 reference statements)

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“…Negative ionization mode was employed, because the matrix influence is reduced in this mode due to the selective ionization of the target phenolic compounds . The average reproducibility of peak areas for the selected ion chronogram of the deprotonated naringenin molecule at m / z 271.0609–271.0618 was ca.…”

Section: Resultsmentioning

confidence: 99%

ID‐CUBE direct analysis in real time high‐resolution mass spectrometry and its capabilities in the identification of phenolic components from the green leaves of Bergenia crassifolia L.

Chernetsova

Crawford²,

Shikov

et al. 2012

Rapid Comm Mass Spectrometry

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Section: Resultsmentioning

confidence: 99%

ID‐CUBE direct analysis in real time high‐resolution mass spectrometry and its capabilities in the identification of phenolic components from the green leaves of Bergenia crassifolia L.

Chernetsova

Crawford²,

Shikov

et al. 2012

Rapid Comm Mass Spectrometry

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“…Ni: not identified.Tr: traces.*a: confirmed by standard; b: confirmed by reference [41, 42]; c: confirmed by MS fragmentation.**d: expressed as cinnamic acid; e: expressed as ferulic acid; f: expressed as caffeic acid; g: expressed as p -coumaric acid; h: expressed as apigenin.…”

Section: Figurementioning

confidence: 98%

Ethanolic Extract of Polish Propolis: Chemical Composition and TRAIL-R2 Death Receptor Targeting Apoptotic Activity against Prostate Cancer Cells

Szliszka

Sokół‐Łętowska

Kucharska

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Propolis possesses chemopreventive properties through direct anticancer and indirect immunomodulatory activities. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) plays a significant role in immunosurveillance and defense against cancer cells. TRAIL triggers apoptosis upon binding to TRAIL-R1 (DR4) and TRAIL-R2 (DR5) death receptors expressed on cancer cell surface. The activation of TRAIL apoptotic signaling is considered an attractive option for cancer prevention. However, as more tumor cells are reported to be resistant to TRAIL-mediated death, it is important to develop new strategies to overcome this resistance. The aim of this study was to investigate the chemical composition and proapoptotic mechanism of ethanolic extract of Polish propolis (EEP-P) against cancer cells. The identification and quantification of phenolic compounds in propolis extract were performed using HPLC-DAD and UPLC-Q-TOF-MS methods. TRAIL-resistant LNCaP prostate cancer cells were treated with EEP-P and TRAIL. Cytotoxicity was measured by MTT and LDH assays. Apoptosis was detected using annexin V-FITC staining by flow cytometry and fluorescence microscopy. Death receptors expression was analyzed using flow cytometry. Pinobanksin, chrysin, methoxyflavanone, p-coumaric acid, ferulic acid and caffeic acid were the main phenolics found in EEP-P. Propolis sensitized LNCaP cells through upregulation of TRAIL-R2. These results suggest that EEP-P supports TRAIL-mediated immunochemoprevention in prostate cancer cells.

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“…For instance, Liu's research team reported its application to the rapid determination of triazine herbicides in water [10]. Chernetsova and collaborators used DART MS to identify phenolic compounds in propolis [14]. Although many types of analytes have been studied, information on analyzing monosaccharides and disaccharides using DART MS is limited [15].…”

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confidence: 99%

Differentiation of Disaccharide Isomers by Temperature-Dependent In-Source Decay (TDISD) and DART-Q-TOF MS/MS

Yang

Shi

Yao

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Helium direct analysis in real time (He-DART) mass spectrometry (MS) of some compounds, polysaccharides, for example, usually tends to be challenging because of the occurrence of prominent in-source decay (ISD), which was considered as an undesired side reaction, as it complicated the resulting mass spectra. Our approach is to take advantage of an efficient and practical method termed the temperature-dependent ISD (TDISD) technique combined with fragmentation of the dehydrated dimers using DART Q-TOF tandem mass spectrometry for differentiation of disaccharide isomers. In this study, cross-ring cleavages and non-ovalent complexes were detected in the spectra of the saccharides. It was observed that the gas heater temperature had a significant effect on the absence or presence of signal in DART spectra. At high gas temperature, ions in high mass region began to appear. Based on the types of crossring cleavages and noncovalent complexes, disaccharide isomers with different linkage positions can be differentiated in both positive and negative ion modes at a lower DART gas temperature. Additionally, anomeric configurations were assigned on the basis of the relative abundance ratio of m/z 198:342 obtained by the comparison of the positive ion mode tandem mass spectrum of an α isomer dimer generated at higher DART gas temperature and that of the corresponding β one. In general, this method is easy, fast, effective, and robust for identifying disaccharide isomers.

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DART-Orbitrap MS: a novel mass spectrometric approach for the identification of phenolic compounds in propolis

Cited by 49 publications

References 19 publications

ID‐CUBE direct analysis in real time high‐resolution mass spectrometry and its capabilities in the identification of phenolic components from the green leaves of Bergenia crassifolia L.

ID‐CUBE direct analysis in real time high‐resolution mass spectrometry and its capabilities in the identification of phenolic components from the green leaves of Bergenia crassifolia L.

Ethanolic Extract of Polish Propolis: Chemical Composition and TRAIL-R2 Death Receptor Targeting Apoptotic Activity against Prostate Cancer Cells

Differentiation of Disaccharide Isomers by Temperature-Dependent In-Source Decay (TDISD) and DART-Q-TOF MS/MS

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