Direct amplification of rRNA gene sequences for identification of selected oral pathogens in root canal infections

Siqueira, José F.; Rôças, Isabela N.; Moraes, Saulo Roni; Santos, Kátia Regina Netto dos

doi:10.1046/j.1365-2591.2002.00485.x

Cited by 57 publications

(44 citation statements)

References 29 publications

(38 reference statements)

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“…were associated with specific signs and symptoms of endodontic origin such as pain, tenderness to percussion, wet canals, and the presence of purulent exudates. Other studies also detected F. nucleatum in symptomatic cases (7) and flare-ups (8). This species is capable of coaggregating with other oral and endodontic species (9), and it has been suggested that it may act as a bridge between earlier and later colonizers in biofilms (10,11).…”

mentioning

confidence: 82%

Frequency, Microbial Interactions, and Antimicrobial Susceptibility of Fusobacterium nucleatum and Fusobacterium necrophorum Isolated from Primary Endodontic Infections

Jacinto

Montagner

Signoretti

et al. 2008

Journal of Endodontics

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mentioning

confidence: 82%

Frequency, Microbial Interactions, and Antimicrobial Susceptibility of Fusobacterium nucleatum and Fusobacterium necrophorum Isolated from Primary Endodontic Infections

Jacinto

Montagner

Signoretti

et al. 2008

Journal of Endodontics

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“…This has been confirmed by studies investigating the microbiologic conditions of root canals associated with persistent AP. 36,37 Presence of persistent cultivable bacteria at the time of obturation reduced the treatment success rate from 94% to 68%. 2 Similarly, success rate in teeth with negative cultures before obturation was significantly higher than teeth with positive cultures.…”

Section: Discussionmentioning

confidence: 99%

The Antibacterial Effect of Additional Photodynamic Therapy in Failed Endodontically Treated Teeth: A Pilot Study

2016

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Introduction: Root canal therapy as a routine dental procedure has resulted in retention of millions of teeth that would otherwise be lost. Unfortunately, successful outcomes are not always achievable within initial endodontic treatments, and that necessitates further treatment. Nonsurgical retreatment is the first choice in most clinical situations. The aim of this clinical pilot study was to assess the effect of additional photodynamic therapy (PDT) on intraradicular bacterial load following retreatment of failed previously root treated teeth. Methods: Thirty single-rooted/canalled endodontically treated matured teeth (in 27 healthy patients) accompanied by apical periodontitis (AP) were selected for this study. Standard protocol was followed for nonsurgical retreatment of each tooth. Microbiological samples were taken after establishment of apical patency, finished cleaning/shaping procedure, and PDT (665 nm, 1 W, 240 seconds). All samples were cultured for 72 hours and colonyforming unit (CFU) was counted. McNemar test was used for statistical analysis of the data. The level of significance was set at 0.001. Results: Routine cleaning and shaping resulted in twenty four negative (80%) out of 30 cultures. Four additional negative results were obtained after additional PDT (93.3%). The addition of PDT to routine procedures significantly enhanced the number of bacteria-free samples (P < 0.001). Conclusion: Regarding elimination of intraradicular microbiota, additional PDT may increase the effectiveness of conventional chemomechanical preparation in previously root filled teeth accompanied by AP. Well controlled randomized clinical trials should be planned for future.

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“…Tannerella.forsythia'nın enfekte kök kanallarında PCR kullanılarak saptanmış prevalansları, çalışmalarda % 4-66 arasında dağılım göstermektedir (1,(5)(6)(7)(8)(9)(10)(11). Bu çalışmada saptanan prevalans % 31 olup aynı PCR tekniği ve örnekleme prosedürü kullanan diğer çalışma-ların bulgularıyla uyum göstermektedir (1,6).…”

Section: Discussionunclassified

“…PCR ile Tannerella forsythia, Treponema denticola, Dialister pneumosintes and Prevotella tannerea gibi mikroorganizmaların kök kanallarında, kültür yön-temleri ile saptanandan çok daha yüksek prevalanslarda bulundukları ortaya çıkmıştır (5,6). PCR kullanılarak yapılan farklı çalışma-larda T.forsythia'nın prevalansı %4-52 arasında değişmektedir (1,(5)(6)(7)(8)(9)(10)(11). Ayrıca bu bakterinin varlığı ile belirli semptomlar arasında bir ilişki-nin bulunup bulunmadığı henüz bir netlik kazanmamıştır.…”

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Primer kok kanal enfeksiyonlarında Tannerella forsythia prevalansının 16S rRNA PCR ile incelenmesi

ULUSOY¹,

Özgür²,

ENGİN³

et al. 2010

Ankara Üniversitesi Diş Hekimliği Fakültesi Dergisi

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The purpose of this study was to assess the prevalence of Tannerella forsythia in the primary infections of root canals and its association with different forms of periradicular diseases using Polymerase Chain Reaction (PCR).Samples were collected from acute and chronic forms of periradicular diseases and abscesses. DNA extraction was performed using spin column DNA isolation kit and 16S rRNA gene based PCR analysis was performed. Chi-squared test was used for data analysis.Tannerella forsythia DNA was detected in 18 (31,3%) of the total 58 samples, in 13 of 26 cases (50%) with acute apical periodontitis, three of seven cases (42,8%) with acute periradicular abscesses, and 2 of 25 cases (8%) with chronic asymptomatic periradicular lesions. It was found to be significantly related with acute apical periodontitis group (p=0.002).T. forsythia must be considered as a potential endodontic pathogen associated with acute symptoms of periradicular disease.

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Direct amplification of rRNA gene sequences for identification of selected oral pathogens in root canal infections

Abstract: Direct molecular approaches appear to be a valuable tool for the rapid and reliable diagnosis of infectious diseases, as well as for research purposes. There was no correlation between target microbial species and symptoms.

Cited by 57 publications

References 29 publications

Frequency, Microbial Interactions, and Antimicrobial Susceptibility of Fusobacterium nucleatum and Fusobacterium necrophorum Isolated from Primary Endodontic Infections

Frequency, Microbial Interactions, and Antimicrobial Susceptibility of Fusobacterium nucleatum and Fusobacterium necrophorum Isolated from Primary Endodontic Infections

The Antibacterial Effect of Additional Photodynamic Therapy in Failed Endodontically Treated Teeth: A Pilot Study

Primer kok kanal enfeksiyonlarında Tannerella forsythia prevalansının 16S rRNA PCR ile incelenmesi

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