Diplotaxis simplex suppresses postprandial hyperglycemia in mice by inhibiting key-enzymes linked to type 2 diabetes

Jdir, Hamida; Khemakham, Bassem; Chakroun, Mouna; Zouari, Sami; Ali, Yassine Ben; Zouari, Nacim

doi:10.1016/j.bjp.2015.02.004

Cited by 16 publications

(20 citation statements)

References 14 publications

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“…Three mechanisms have been proposed: the viscosity increase in the intestine to hinder the glucose diffusion, glucose uptake, and the retardation of α-amylase activity. [ 13 14 21 ] Taking into account our previous study which revealed that C. limetta peel flour has high ability to inhibit α-glucosidase and α-amylase and now the in vitro results showed capacity to glucose adsorption and its retardation, we suspected that C. limetta peel flour sample plays a role in lowering postprandial serum glucose level in the intestinal lumen as other fibers. [ 18 ]…”

Section: Resultsmentioning

confidence: 99%

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In vitro and In vivo postprandial glycemic activity of Citrus limetta peel flour

et al. 2017

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Background:Previous studies of Citrus spp. peel have shown hypoglycemic and antioxidant activities. Citrus limetta has been studied for its therapeutic properties. Diabetes mellitus (DM) is a health problem in Mexico and worldwide, that takes a vital importance due to its high incidence. Recently, scientists have searched natural sources to control the disease.Materials and Methods:In this study, we evaluated the in vitro hypoglycemic activity and in vivo postprandial glycemic effect of C. limetta peel flour by glucose adsorption and retardation assays as well as postprandial serum glucose levels using a group of female Balb-c mice, respectively.Results:C. limetta peel flour showed a glucose adsorption capacity of 16.58 mM, having a similar effect regarding the positive control. The glucose diffusion in the dialysate was elevated, with a glucose dialysis retardation index of 33.79% in a period of 3 h, showing similar results to positive control. Postprandial serum glucose levels in the animal group treated with C. limetta peel flour showed a glucose level of 41.4 mg/dL, being this value significantly lower than negative control group and similar to positive control. Toxicity tests showed good tolerance to the dose of 2000 mg/kg.Conclusion:C. limetta peel flour could act as a source of functional compounds for the control of DM.SUMMARY Citrus limetta peel flour showed a glucose adsorption capacity similar to the positive controlThe glucose diffusion in the dialysate was elevated, showing similar results to positive controlPostprandial serum glucose levels in the animal group treated with C. limetta peel flour showed a glucose level significantly lower than negative control group and similar to positive controlToxicity tests showed good toleranceC. limetta peel flour could act as a source of functional compounds for the control of diabetes mellitus. Abbreviations used: CIATEJ: Center for Research and Assistance in Technology and Design of Jalisco; DM: Diabetes mellitus; FGC: Final glucose concentration; GDRI: Glucose dialysis retardation index; IGC: Initial glucose concentration; OECD: Organization for Economic Cooperation and Development.

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Section: Resultsmentioning

confidence: 99%

“…Blood samples were collected from the mice tail vein before and 30 min after maltose administration, and glucose levels were measured based on the glucose oxidase method using a glucometer (ACCU-CHEK Meter ® , Roche Diagnostics Corp., USA). [ 13 ]…”

Section: Methodsmentioning

confidence: 99%

In vitro and In vivo postprandial glycemic activity of Citrus limetta peel flour

et al. 2017

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“…In fact, these herbs are appreciated for their strong pungent flavour and they are consumed raw or cooked, in salads and soups (Zouari 2015). Recently, it was shown that this species suppresses postprandial hyperglycaemia in mice by inhibiting key-enzymes linked to type 2 diabetes (Jdir et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory and anti-proliferative activities of the wild edible cruciferous:Diplotaxis simplex

Jdir

Khemakham

Najjaa

et al. 2016

Pharmaceutical Biology

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Context The present study deals with new biological properties of the wild edible Diplotaxis simplex (Viv.) Spreng (Brassicaceae). Objectives The current study evaluates the antioxidant, the anti-inflammatory and the anti-cancer properties of ethyl acetate and ethanol extracts from D. simplex flowers. Materials and methods The anti-proliferative activity of the extracts (10-70 mg/mL) was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) against human colon cancer cell line Caco-2. The anti-inflammatory potential was evaluated by the inhibitory effect of the extracts (1.5-7.5 mg/mL) on phospholipase A2 activity as well as on carrageenan-induced paw oedema in mice. Extracts (200 mg/kg) or indomethacin (50 mg/kg) as positive control were injected intraperitoneally for albino mice prior to the induction of the oedema by carrageenan. Antioxidant activities were investigated using various complementary methods.Results Flower extracts contained a high level of polyphenolics (17.10-52.70 mg GAE/g) and flavonoids (74.20-100.60 mg QE/g), which correlate with its appreciable antioxidant potential in b-carotene peroxidation (IC 50 value: 12.50-27.10 mg/mL), DPPH radical-scavenging (IC 50 value: 0.20-0.40 mg/mL), Fe 3+ reducing (EC 50 value: 0.10-0.14 mg/mL) and Fe 2+ chelating (IC 50 value: 0.20-0.60 mg/mL) assays. These extracts were effective in inhibiting cancer cell growth (IC 50 value: 62.0-63.25 mg/mL). Besides, the ethyl acetate extract inhibited phospholipase A2 activity (IC 50 value: 2.97 mg/mL) and reduced the paw oedema in mice (from 0.38 ± 0.01 to 0.24 ± 0.01 cm), 4 h postcarrageenan challenge. Conclusion These data suggest that D. simplex may be useful as a candidate in the treatment of inflammation and the colon cancer.ARTICLE HISTORY

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“…which has been demonstrated to provide protection against dia-betes by preventing the formation of endogenous aldehydes and removal of reactive oxygen species generated by auto-oxidation of glucose [25][26][27][28]. The glucosidase inhibitors such as acarbose and migliotol inhibit enzymes responsible for the breakdown of carbohydrates in the small intestine [29]. They were classified in the group of antihyperglycemic drugs that are often administered to patients with diabetes type 2 before meals to control postprandial glucose levels [30].…”

Section: Discussionmentioning

confidence: 99%

Anti-α-glucosidase and antiglycation activities of galls from Guiera senegalensis J.F. Gmel (combretaceae)

Sombié¹,

Hafizur

Compaoré

et al. 2018

ijpm

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The hypoglycemic activity of Guiera senegalensis used in Burkinabe folk medicine has been already reported. The aim of this study was to investigate the in vitro antidiabetic activity from galls of G.senegalensis. The extracts and methanol fractions from galls of G. senegalensis showed strong α-glucosidase inhibitory activity compared with acarbose. The ethyl acetate fraction from methanol extract (EA/ME) showed potent antiglycation activity in an in vitro assay system. The galls did not show inhibition activity against α-chymotrypsin. The α-glucosidase inhibitory activity along with its antiglycation activity may open a new perspective for the use of G. senegalensis for the diabetic subject. The data suggests that consumption of G senegalensis galls as an infusion or in food and pharmaceutical preparations may be useful for the management of diabetes and its complications.

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Diplotaxis simplex suppresses postprandial hyperglycemia in mice by inhibiting key-enzymes linked to type 2 diabetes

Cited by 16 publications

References 14 publications

In vitro and In vivo postprandial glycemic activity of Citrus limetta peel flour

In vitro and In vivo postprandial glycemic activity of Citrus limetta peel flour

Anti-inflammatory and anti-proliferative activities of the wild edible cruciferous:Diplotaxis simplex

Anti-α-glucosidase and antiglycation activities of galls from Guiera senegalensis J.F. Gmel (combretaceae)

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