Dipeptidyl peptidase‐IV inhibitory potential of alpha‐lactalbumin extracted from milk of Gir cows: A Bos indicus species

Abstract

An attempt has been made to assess the dipeptidyl peptidase‐IV (DPP‐IV) inhibitory potential of α‐lactalbumin separated from milk of Gir cow. α‐lactalbumin hydrolysates were prepared using three different enzymes at different enzyme‐to‐substrate (E:S) ratios (1:25, 1:50 and 1:100) treated for 2‐ to 12‐h durations. Pepsin‐treated hydrolysates showed maximum DPP‐IV inhibition of 87.81 ± 0.84% (IC50 = 0.78 mg/mL). The permeate of 3 kDa, which exhibited 84.47 ± 2.29% DPP‐IV inhibition, was fractionated through RP‐HPLC. All fractions were analysed for DPP‐IV inhibition activity. One novel peptide was identified through LC‐MS/MS, which showed adequate DPP‐IV inhibition (IC50 = 4.93 mM).

Encapsulation of dipeptidyl peptidase‐IV inhibitory peptides from alpha‐lactalbumin extracted from milk of Gir cows – A Bos indicus species

“…The respective protein contents were 73.75 AE 1.09% and 68.59 AE 0.94%. Kumar et al (2022) reported 72.91 AE 1.91% protein in the freeze dried sample. The percent DPP-IV inhibition of hydrolysate obtained from α-lactalbumin is shown in Figure 2.…”

“…The enzymatic hydrolysis of α-lactalbumin was done using pepsin at 1:50 enzyme-substrate ratio using the method standardised at the author's laboratory (Kumar et al 2022). Dipeptidyl peptidase-IV inhibitory activity of hydrolysate of α-lactalbumin was determined in triplicate by the method described by Lacroix and Li-Chan (2013) as elaborated in Kumar et al (2022).…”

“…The enzymatic hydrolysis of α-lactalbumin was done using pepsin at 1:50 enzyme-substrate ratio using the method standardised at the author's laboratory (Kumar et al 2022). Dipeptidyl peptidase-IV inhibitory activity of hydrolysate of α-lactalbumin was determined in triplicate by the method described by Lacroix and Li-Chan (2013) as elaborated in Kumar et al (2022). The degree of hydrolysis of the whey protein hydrolysate samples at different time intervals and enzyme substrate ratios was analysed in triplicate by ophthalaldehyde (OPA) method developed by Nielsen et al (2001) and as detailed in Kumar et al (2022).…”

“…A recent study conducted by Kumar et al . (2022) reported that peptides obtained by hydrolysis of α‐lactalbumin using pepsin showed considerable DPP‐IV inhibition. Encapsulation of peptides helps to protect them from environmental conditions and also facilitates targeted delivery and control release action.…”

“…Uchida et al (2011) isolated peptides from trypsin-treated βlactoglobulin hydrolysate, which showed a concentration-dependent DPP-IV inhibition with an IC50 value of 210 μM/g. A recent study conducted by Kumar et al (2022) reported that peptides obtained by hydrolysis of α-lactalbumin using pepsin showed considerable DPP-IV inhibition. Encapsulation of peptides helps to protect them from environmental conditions and also facilitates targeted delivery and control release action.…”