Dipeptidyl peptidase 4 as a potential serum biomarker for disease activity and treatment response in rheumatoid arthritis

Yu, Jiahui; Hu, Congqi; Dai, Zhao; Xu, Jia; Zhang, Lu; Deng, Hui; Xu, Yanping; Zhao, Lianyu; Li, Meilin; Liu, Lijuan; Zhang, Mingying; Huang, Jiarong; Wu, Lin-Ping; Chen, Guangxing

doi:10.1016/j.intimp.2023.110203

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…Serum samples were collected from patients with CRSwNP in the discovery cohort, and serum proteomics analysis was conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS) as previously outlined. 20 , 29 Briefly, serum samples were processed to extract total proteins, and their concentration was determined using the BCA assay. Subsequently, 100 μg of protein underwent acetone precipitation, followed by redissolution, reduction, alkylation, protein digestion, and final peptide desalting.…”

Section: Methodsmentioning

confidence: 99%

Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

Chen,

Gao,

Liu

et al. 2024

JIR

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Background Individuals with eosinophilic chronic rhinosinusitis with nasal polyps(eCRSwNP) exhibited worse outcomes and higher postoperative recurrence rates. This study aimed to identify biomarkers that can aid in the early differentiation of eCRSwNP and enhance our comprehension of its pathophysiology. Methods We recruited two independent cohorts. In the discovery cohort, CRSwNP was categorized into eCRSwNP and non-eosinophilic CRSwNP(neCRSwNP), and serum proteomics was performed to identify differentially expressed proteins between the two groups. These candidate proteins were chosen and confirmed in the validation cohort using an enzyme-linked immunosorbent assay (ELISA), Western blot (WB), quantitative real time-polymerase chain reaction (qRT-PCR), immunofluorescence (IF), and their predictive values and associations with tissue eosinophilic pathophysiology were evaluated. Results We identified a total of 39 differential proteins between the two groups, including 20 proteins upregulated and 19 downregulated in the eCRSwNP group. Further validation was conducted on the top 5 proteins that were up or down-regulated. Results from the ELISA showed that levels of serum MRC1, CDH13, and MMP2 were significantly higher, TRIM28 was lower in the eCRSwNP group compared to the neCRSwNP group (all P<0.05), and serum MRC1 (AUC=0.742, P<0.001) and MMP2 (AUC=0.766, P<0.001) levels exhibited promising predicting values for eCRSwNP. Moreover, qRT-PCR and WB analysis found that MMP2 and MRC1 expressions were enhanced in the eCRSwNP group compared to the neCRSwNP group (all P<0.01), and their levels were positively correlated with the number and percentages of tissue eosinophils (all P<0.01). The IF suggested that MMP2 and MRC1 were overexpressed in the nasal polyps tissues of eCRSwNP patients, and MMP2 was mainly located on eosinophils. Conclusion Circulating proteins identified by proteomics could serve as potential preoperative biomarkers for distinguishing eCRSwNP. Among them, MMP2 was enhanced in eCRSwNP and correlated with tissue eosinophilia, which provided valuable insights into the pathophysiology of eCRSwNP.

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Section: Methodsmentioning

confidence: 99%

Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

Chen,

Gao,

Liu

et al. 2024

JIR

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“…However, the types of arthritis treated with these targets are not completely clear. Targets, β2-adrenergic receptor (ADRB2), AHR, CRP, IRF1, prostaglandin G/H synthase 1 (PTGS1), SPP1 and other targets, exert key effects in the pathogenesis of arthritis, but role of them in the treatment of arthritis with CC has not been confirmed ( Table 3 ) ( Xu et al, 2004 ; Koskela et al, 2012 ; Ko et al, 2013 ; Li et al, 2013 ; Xue et al, 2014 ; Song et al, 2016 ; Trzybulska et al, 2018 ; Bonelli et al, 2019 ; Chu et al, 2019 ; Lin et al, 2019 ; Sun et al, 2020 ; Orecchini et al, 2020 ; Yang et al, 2020 ; Nalesso et al, 2021 ; Tang et al, 2021 ; Xu and Xu, 2021 ; Zou et al, 2021 ; Balasundaram et al, 2022 ; Cai et al, 2022 ; Liu et al, 2022 ; Wang et al, 2022 ; Zhang et al, 2022 ; Zhang et al, 2022 ; Han et al, 2022 ; Man et al, 2022 ; Nguyen et al, 2022 ; Qian et al, 2022 ; Tavallaee et al, 2022 ; Zhuang et al, 2022 ; Afnan et al, 2023 ; Baggio et al, 2023 ; Sun et al, 2023b ; Zhou et al, 2023 ; Fan et al, 2023 ; Huang et al, 2023 ; Ning et al, 2023 ; Qin et al, 2023 ; Skougaard et al, 2023 ; Tu et al, 2023 ; Xie et al, 2023 ; Yan et al, 2023 ; Yu et al, 2023 ; Zhao et al, 2023 ). We have elaborated on the role of targets such as MMP-3, IL-1β, MAPK, IL-6, ADRB2, AHR, CRP, CAV1, CXCL2, SPP1 and other targets in treating arthritis, explored the potential targets and mechanisms of CC in treating arthritis, analyzed the feasibility of CC as an anti-arthritis drug, and provided a theoretical basis for subsequent research.…”

Section: Deep Exploration Based On Potential Therapeutic Targets For ...mentioning

confidence: 99%

Therapeutic potential of Coptis chinensis for arthritis with underlying mechanisms

Tian

Guo

et al. 2023

Front. Pharmacol.

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Arthritis is a common degenerative disease of joints, which has become a public health problem affecting human health, but its pathogenesis is complex and cannot be eradicated. Coptis chinensis (CC) has a variety of active ingredients, is a natural antibacterial and anti-inflammatory drug. In which, berberine is its main effective ingredient, and has good therapeutic effects on rheumatoid arthritis (RA), osteoarthritis (OA), gouty arthritis (GA). RA, OA and GA are the three most common types of arthritis, but the relevant pathogenesis is not clear. Therefore, molecular mechanism and prevention and treatment of arthritis are the key issues to be paid attention to in clinical practice. In general, berberine, palmatine, coptisine, jatrorrhizine, magnoflorine and jatrorrhizine hydrochloride in CC play the role in treating arthritis by regulating Wnt1/β-catenin and PI3K/AKT/mTOR signaling pathways. In this review, active ingredients, targets and mechanism of CC in the treatment of arthritis were expounded, and we have further explained the potential role of AHR, CAV1, CRP, CXCL2, IRF1, SPP1, and IL-17 signaling pathway in the treatment of arthritis, and to provide a new idea for the clinical treatment of arthritis by CC.

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Serum proteomics identifies S100A11 and MMP9 as novel biomarkers for predicting the early efficacy of sublingual immunotherapy in allergic rhinitis

Zhang,

Shui,

Wang

et al. 2023

International Immunopharmacology

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Dipeptidyl peptidase 4 as a potential serum biomarker for disease activity and treatment response in rheumatoid arthritis

Cited by 4 publications

References 40 publications

Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

Therapeutic potential of Coptis chinensis for arthritis with underlying mechanisms

Serum proteomics identifies S100A11 and MMP9 as novel biomarkers for predicting the early efficacy of sublingual immunotherapy in allergic rhinitis

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