Dipeptidyl Aminopeptidase IV Staining of Cytologic Preparations to Distinguish Benign from Malignant Thyroid Diseases

Aratake, Yatsuki; Kotani, Takuya; Tamura, Kazuo; Araki, Yasunori; Kuribayashi, Tadanobu; Konoe, K.; Ohtaki, Sachiya

doi:10.1093/ajcp/96.3.306

Cited by 69 publications

(67 citation statements)

References 16 publications

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“…Thyroid peroxidase ICC using MoAb 47 and DPP4-activation detection were the first malignancy markers reported to have a diagnostic significance on thyroid FNA smears (Aratake et al, 1992;De Micco et al, 1994a). In large series, TPO has been shown to have Se of 98% and Sp of 80% (Faroux et al, 1997;De Micco et al, 1998;Christensen et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Staining was scored using a discontinuous 0 -12-point scale as described previously (Aratake et al, 1992). Positive controls were performed on smears from operated malignant nodules.…”

Section: Dpp4 Cytoenzymologymentioning

confidence: 99%

“…Encouraging results have been obtained using DPP4 CE for diagnosis of thyroid malignancy on FNA material (Aratake et al, 1992;Zoro et al, 1996). A high-contrast red stain appears on the cellular membrane in malignant thyroid cells.…”

mentioning

confidence: 99%

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Utility of malignancy markers in fine-needle aspiration cytology of thyroid nodules: comparison of Hector Battifora mesothelial antigen-1, thyroid peroxidase and dipeptidyl aminopeptidase IV

Micco¹,

Савченко²,

Giorgi

et al. 2008

Br J Cancer

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The purpose of this study was to compare the diagnostic interest of Hector Battifora mesothelial antigen-1 (HBME-1), thyroid peroxidase (TPO), and dipeptidyl aminopeptidase IV (DPP4) in thyroid fine-needle aspirates obtained from 200 resected thyroid lesions (55 colloid nodules, 54 follicular adenomas, 59 papillary cancers, and 32 follicular carcinomas). Hector Battifora mesothelial antigen-1 or TPO expression (% positive cells) and DPP4 staining score (12-point scale) were evaluated. Receiver operating characteristic (ROC) curves were plotted and optimal cutoff values for diagnosing malignancy were determined. The TPO ROC curve was consistently higher than the HBME-1 ROC curve. The TPO curve was also higher than the DPP4 curve with regard to sensitivity, but dipped below the DPP4 curve with regard to specificity. Using a cutoff value of o80% positive cells for TPO, 410% positive cells for HBME-1, and staining score X1 for DPP4, sensitivity to specificity ratios were 98 -83% for TPO, 90 -60% for HBME-1, and 88 -80% for DPP4. Two particularly interesting findings of this study were the low negative likelihood ratio of TPO (0.02) allowing highly reliable exclusion of malignancy and the 100% specificity of DPP4 staining scores ¼ 12. Due to poor performance on follicular lesions, HBME-1 showed no advantage over TPO or DPP4.

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Section: Discussionmentioning

confidence: 99%

“…Staining was scored using a discontinuous 0 -12-point scale as described previously (Aratake et al, 1992). Positive controls were performed on smears from operated malignant nodules.…”

Section: Dpp4 Cytoenzymologymentioning

confidence: 99%

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Utility of malignancy markers in fine-needle aspiration cytology of thyroid nodules: comparison of Hector Battifora mesothelial antigen-1, thyroid peroxidase and dipeptidyl aminopeptidase IV

Micco¹,

Савченко²,

Giorgi

et al. 2008

Br J Cancer

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“…Studies of patient samples showed that CD26 is highly expressed on lung adenocarcinoma, thyroid carcinoma, and B-cell chronic lymphocytic leukemia (6)(7)(8). Meanwhile, CD26 is a marker of aggressive disease for selected subsets of T-cell non-Hodgkin's lymphomas/ leukemias.…”

Section: Introductionmentioning

confidence: 99%

CD26 Regulates p38 Mitogen-Activated Protein Kinase–Dependent Phosphorylation of Integrin β1, Adhesion to Extracellular Matrix, and Tumorigenicity of T-Anaplastic Large Cell Lymphoma Karpas 299

et al. 2005

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CD26 is an antigen with key role in T-cell biology and is expressed on selected subsets of aggressive T-cell malignancies. To elucidate the role of CD26 in tumor behavior, we examine the effect of CD26 depletion by small interfering RNA transfection of T-anaplastic large cell lymphoma Karpas 299. We show that the resultant CD26-depleted clones lose the ability to adhere to fibronectin and collagen I. Because antiintegrin B 1 blocking antibodies also prevent binding of Karpas 299 to fibronectin and collagen I, we then evaluate the CD26-integrin B 1 association. CD26 depletion does not decrease integrin B 1 expression but leads to dephosphorylation of both integrin B 1 and p38 mitogen-activated protein kinase (MAPK). Moreover, our data showing that the p38MAPK inhibitor SB203580 dephosphorylates integrin B 1 and that binding of the anti-CD26 antibody 202.36 dephosphorylates both p38MAPK and integrin B 1 on Karpas 299, leading to loss of cell adhesion to the extracellular matrix, indicate that CD26 mediates cell adhesion through p38MAPK-dependent phosphorylation of integrin B 1 . Finally, in vivo experiments show that depletion of CD26 is associated with loss of tumorigenicity and greater survival. Our findings hence suggest that CD26 plays an important role in tumor development and may be a novel therapeutic target for selected neoplasms. (Cancer Res 2005; 65(15): 6950-6)

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“…It plays an important role in T-cell biology through its physical and functional association with molecules involved in T-cell signal transduction processes (1)(2)(3)(4)(5)(6). Recent findings suggest that CD26/DPPIV has a role in the development of certain neoplasms, being overexpressed in certain aggressive T-cell malignancies (7,8), B-chronic lymphocytic leukemia (9), and thyroid carcinoma (10). On the other hand, loss or decreased surface expression of CD26/DPPIV is found in prostate cancer (11), colorectal carcinoma (12), and melanomas (13).…”

Section: Introductionmentioning

confidence: 99%

Regulation of p38 Phosphorylation and Topoisomerase IIα Expression in the B-Cell Lymphoma Line Jiyoye by CD26/Dipeptidyl Peptidase IV Is Associated with EnhancedIn vitroandIn vivoSensitivity to Doxorubicin

Yamochi

Aytac²,

Sato³

et al. 2005

Cancer Research

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CD26 is a M r 110,000 surface-bound glycoprotein with diverse functional properties, including having a key role in normal T-cell physiology and the development of certain cancers. In this article, we show that surface expression of CD26, especially its intrinsic dipeptidyl peptidase IV (DPPIV) enzyme activity, results in enhanced topoisomerase IIA level in the B-cell line Jiyoye and subsequent in vitro sensitivity to doxorubicininduced apoptosis. In addition, we show that expression of CD26/DPPIV is associated with increased phosphorylation of p38 and its upstream regulators mitogen-activated protein kinase kinase 3/6 and apoptosis signal-regulating kinase 1 and that p38 signaling pathway plays a role in the regulation of topoisomerase IIA expression. Besides demonstrating that CD26 effect on topoisomerase IIA and doxorubicin sensitivity is applicable to cell lines of both B-cell and T-cell lineages, the potential clinical implication of our work lies with the fact that we now show for the first time that our in vitro results can be extended to a severe combined immunodeficient mouse model. Our findings that CD26 expression can be an in vivo marker of tumor sensitivity to doxorubicin treatment may lead to future treatment strategies targeting CD26/DPPIV for selected human cancers in the clinical setting. Our article thus characterizes the biochemical linkage among CD26, p38, and topoisomerase IIA while providing evidence that CD26-associated topoisomerase IIA expression results in greater in vitro and in vivo tumor sensitivity to the antineoplastic agent doxorubicin. (Cancer Res 2005; 65(5): 1973-83)

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Dipeptidyl Aminopeptidase IV Staining of Cytologic Preparations to Distinguish Benign from Malignant Thyroid Diseases

Cited by 69 publications

References 16 publications

Utility of malignancy markers in fine-needle aspiration cytology of thyroid nodules: comparison of Hector Battifora mesothelial antigen-1, thyroid peroxidase and dipeptidyl aminopeptidase IV

Utility of malignancy markers in fine-needle aspiration cytology of thyroid nodules: comparison of Hector Battifora mesothelial antigen-1, thyroid peroxidase and dipeptidyl aminopeptidase IV

CD26 Regulates p38 Mitogen-Activated Protein Kinase–Dependent Phosphorylation of Integrin β1, Adhesion to Extracellular Matrix, and Tumorigenicity of T-Anaplastic Large Cell Lymphoma Karpas 299

Regulation of p38 Phosphorylation and Topoisomerase IIα Expression in the B-Cell Lymphoma Line Jiyoye by CD26/Dipeptidyl Peptidase IV Is Associated with EnhancedIn vitroandIn vivoSensitivity to Doxorubicin

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