Dimethyl suberimidate cross-linked pericardium tissue: Raman spectroscopic and atomic force microscopy investigations

Effect of cross-linking with riboflavin and ultraviolet A on the chemical bonds and ultrastructure of human sclera

“…This method was used successfully to examine the mechanism of glutaraldehyde or dimethyl suberimidate cross-linking in pericardium tissue. 22,23 Atomic force microscopy (AFM) is a promising tool for observing the surface topography or nanostructure of biological tissues because it provides high spatial resolution on the nanometer scales as well as the three-dimensional ultrastructure of the surface morphology. 24,25 Therefore, this study investigated the effect of cross-linking with riboflavin-UVA irradiation on the chemical bonds and ultrastructure of human sclera tissues using Raman spectroscopy, AFM, and histology.…”

Inhibition of calcification of bovine pericardium after treatment with biopolymers, E-beam irradiation and in vitro endothelization

“…3 shows the Raman spectra for the treatments made on BP native with chitosan, silk fibroin (at three different proportions 1:3, 1:1 and 3:1) and E-beam irradiation. As noted, the spectral shape of BP is largely preserved, recognizing for example, collagen characteristic peaks regions of the amide III (1246 cm −1 ), the molecular vibration mode deformation to NH (approximately 1450 cm −1 ) and the amide I region (about 1665 cm −1 ) [58][59][60]. The preservation of these bands indicates that the collagen structure is preserved.…”

Novel hydrogels based on carboxyl pullulan and collagen crosslinking with 1, 4-butanediol diglycidylether for use as a dermal filler: initial in vitro and in vivo investigations

“…HLC has been used in hydrogels [10,11], vascular scaffolds [12] and artificial bones [13] for tissue engineering applications. A crosslinking agent is necessary for conjugate composition, and several types of crosslinker have been reported, such as dimethyl suberimidate [14], glyoxal [15], genipin [16] and EDC [17]. However, the classical crosslinker BDDE is still in use, as it is consistent with FDA/EMEA regulations.…”