Inhibition of calcification of bovine pericardium after treatment with biopolymers, E-beam irradiation and in vitro endothelization

Polak, Roberta; Rodas, Andrea C.D.; Chicoma, Dennis L.; Giudici, Reinaldo; Beppu, Marisa Masumi; Higa, Olga Zazuco; Pitombo, Ronaldo N.M.

doi:10.1016/j.msec.2012.08.009

Cited by 13 publications

(8 citation statements)

References 61 publications

(70 reference statements)

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“…57 Moreover, xenogeneic antigens are masked and thus prevent foreign body reactions. 58 Furthermore, endothelial cells have shown protective effects against tissue calcification. 58 Scattered endothelial cells, as on glutaraldehyde-treated pericardia, are not sufficient to evoke those beneficial effects.…”

Section: Discussionmentioning

confidence: 99%

“…58 Furthermore, endothelial cells have shown protective effects against tissue calcification. 58 Scattered endothelial cells, as on glutaraldehyde-treated pericardia, are not sufficient to evoke those beneficial effects. In contrast, a confluent endothelial cell layer on riboflavin/UV–LEEI-treated pericardia may contribute to prolonging the tissue's durability in vivo .…”

Section: Discussionmentioning

confidence: 99%

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Stabilization and Sterilization of Pericardial Scaffolds by Ultraviolet and Low-Energy Electron Irradiation

Walker

Schönfelder

Tugtekin

et al. 2018

Tissue Engineering Part C: Methods

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Pericardial scaffolds have a wide spectrum of clinical applications ranging from patches for vascular reconstruction and abdominal wall defects to bioprosthetic heart valves. The current gold standard of tissue preparation involves disinfection and cross-linking using glutaraldehyde. However, glutaraldehyde-associated toxicity as well as rapid calcification and premature graft failure represent the major modes of failure.1 Therefore, a variety of alternative strategies for tissue conservation have been pursued. However, none of those strategies has substituted glutaraldehyde as the method of choice yet. Furthermore, safe sterilization procedures that are nondetrimental to the tissue's functionality are scarce. We have developed a novel procedure to stabilize and sterilize (S) acellular pericardial scaffolds combining photo-initiated ultraviolet cross-linking (U) with low-energy electron irradiation (LEEI). This SULEEI procedure avoids the use of glutaraldehyde and utilizes LEEI as effective sterilization method. A bioburden of 5.1 × 105 ± 4.6 × 105 viable bacteria could be successfully inactivated by SULEEI treatment applying a surface dose of 30.6 ± 2.8 kGy. By challenging high-density polyethylene foil stacks with >106 Bacillus pumilus spores in different depths and modeling the dose distribution within the scaffolds, a maximum sample thickness of 175 μm was determined for successful sterilization. Moreover, SULEEI treatment appeared nondetrimental to the ultimate tensile strength (17.6 ± 8.6 MPa vs. 17.4 ± 9.6 MPa) of the scaffolds compared with glutaraldehyde-treated pericardia. Cell number and overall metabolic activity of human endothelial cells were significantly higher on SULEEI-treated pericardia compared with control samples. In contrast, no cell proliferation could be detected on glutaraldehyde-treated pericardia. Thus, the SULEEI procedure may be a promising novel procedure for glutaraldehyde-free tissue preparation for pericardium-based tissue transplants and tissue engineering.Impact StatementPericardium-based tissue transplantation is a lifesaving treatment. Commercial glutaraldehyde-treated pericardial tissue exhibits cytotoxicity, which is associated with the accelerated graft failure. Replacement of glutaraldehyde has been suggested to overcome those drawbacks. In this study, we report a toxin-free method that combines tissue stabilization with a terminal sterilization. Our data indicate that the SULEEI procedure, which is part of an issued patent, may be a promising first step toward glutaraldehyde-free pericardium-based tissue transplants. Thus, our results may contribute to improving cardiovascular treatment strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Stabilization and Sterilization of Pericardial Scaffolds by Ultraviolet and Low-Energy Electron Irradiation

Walker

Schönfelder

Tugtekin

et al. 2018

Tissue Engineering Part C: Methods

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“…In order to mimic in vitro physiological environment and stimulate the mineralization into the BP, the samples were immersed in SBF. This solution has been used by many authors in similar studies of surface treatment in BP in order to simulate in vitro the calcification process . Other authors have chosen methods such as experimenting on mice .…”

Section: Methodsmentioning

confidence: 99%

“…This solution has been used by many authors in similar studies of surface treatment in BP in order to simulate in vitro the calcification process. 3,9,13,14 Other authors have chosen methods such as experimenting on mice. 12 We have chosen to perform the in vitro approach because the results presented in the literature show that this enables the study of calcification in BP avoiding the use of in vivo assays.…”

Section: Methodsmentioning

confidence: 99%

Mechanical behavior of bovine pericardium treated with hyaluronic acid derivative for bioprosthetic aortic valves

et al. 2019

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We studied the mechanical behavior of bovine pericardium (BP) after anticalcification treatment using hyaluronic acid (HA) derivative. To simulate the physiological environment and stimulate the calcification process, the BP samples were immersed into simulated body fluid solution. We conducted scanning electron microscopy with energy dispersive X‐ray spectrometry, and uniaxial mechanical tests of HA‐treated and non‐treated samples. Although our microstructural analyses indicated that the HA treatment actually prevents the formation of calcium phosphate deposits, the mechanical tests show significant increase of stiffness of the HA‐treated samples. Using data from our mechanical tests as input parameters, we performed finite element (FE) computer simulations to estimate how this increase in the BP stiffness affects the stress distribution in the bioprosthetic leaflet. Although the maximum stress observed during the closing phase of the membrane in vivo is below the experimental yield stress in all cases we analyzed, our FE results indicate that increase of BP stiffness due to HA anticalcification treatment results in higher risk of disruption and failure of the leaflets in bioprosthetic heart valves. Since our FE results indicate that the commissure and the fixed edge are the regions that withstand the highest mechanical stresses during the closing phase, new designs of the valve might be efficient to enhance the endurance of the prosthesis. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2273–2280, 2019.

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“…Forming of an endothelial monolayer takes several weeks following implantation of an acellular ECM scaffold 25 . Complete endothelialization is associated with reduced incidence of thrombosis and calcification 27 , making rapid formation of a quiescent endothelial monolayer a primary concern for the development of tissue engineered scaffolds. Similarly, in valvular applications, in vitro endothelialization of xenografts prior to implantation improves ultimate in vivo endothelial coverage 28 , valve durability and reduces tissue degeneration 29,30 .…”

Section: Introductionmentioning

confidence: 99%

Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function

Shklover

McMasters

Alfonso-García

et al. 2019

Sci Rep

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Xenogeneic biomaterials contain biologically relevant extracellular matrix (ECM) composition and organization, making them potentially ideal surgical grafts and tissue engineering scaffolds. Defining the effect of ECM niche (e.g., basement membrane vs. non-basement membrane) on repopulating cell phenotype and function has important implications for use of xenogeneic biomaterials, particularly in vascular applications. We aim to understand how serous (i.e., basement membrane) versus fibrous (i.e., non-basement membrane) ECM niche of antigen-removed bovine pericardium (AR-BP) scaffolds influence human aortic endothelial cell (hAEC) adhesion, growth, phenotype, inflammatory response and laminin production. At low and moderate seeding densities hAEC proliferation was significantly increased on the serous side. Similarly, ECM niche modulated cellular morphology, with serous side seeding resulting in a more rounded aspect ratio and intact endothelial layer formation. At moderate seeding densities, hAEC production of human laminin was enhanced following serous seeding. Finally, inflammatory marker and pro-inflammatory cytokine expression decreased following long-term cell growth regardless of seeding side. This work demonstrates that at low and moderate seeding densities AR-BP sidedness significantly impacts endothelial cell growth, morphology, human laminin production, and inflammatory state. These findings suggest that ECM niche has a role in modulating response of repopulating recipient cells toward AR-BP scaffolds for vascular applications.

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Inhibition of calcification of bovine pericardium after treatment with biopolymers, E-beam irradiation and in vitro endothelization

Cited by 13 publications

References 61 publications

Stabilization and Sterilization of Pericardial Scaffolds by Ultraviolet and Low-Energy Electron Irradiation

Stabilization and Sterilization of Pericardial Scaffolds by Ultraviolet and Low-Energy Electron Irradiation

Mechanical behavior of bovine pericardium treated with hyaluronic acid derivative for bioprosthetic aortic valves

Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function

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