Digitalis

Sales, Ester; Müller‐Uri, Frieder; Nebauer, Sergio G.; Segura, Juan; Kreis, Wolfgang; Arrillaga, Isabel

doi:10.1007/978-3-642-21201-7_5

Cited by 9 publications

(10 citation statements)

References 208 publications

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“…Therefore, plants regenerated using the protocol described above could be used as a raw material for cardenolide production. This production in Digitalis plants, has proved to be affected by environmental factors, developmental stage as well as method of propagation (Sales et al, 2011). Knowledge of the biosynthetic pathway is limited and there is no report on the interaction of plant growth regulators and production of these compounds.…”

Section: These Results Indicate the Presence Ofmentioning

confidence: 99%

“…Nevertheless, multiple factors modulate cardenolide concentration in plants cultivated in the field, e.g. temperature, mineral soil composition, season, humidity and others (Sales et al, 2011). Furthermore, wild populations of Digitalis species are significantly affected by large-scale and uncontrolled exploitation in order to satisfy the pharmaceutical industry (Verma et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Efﬁcient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.

Pérez-Alonso

Martín

Capote

et al. 2018

Industrial Crops and Products

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Section: These Results Indicate the Presence Ofmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efﬁcient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.

Pérez-Alonso

Martín

Capote

et al. 2018

Industrial Crops and Products

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“…Today, D. purpurea L. and, more notably, D. lanata Ehrh. are both widely collected from nature and field-cultivated to extract cardenolides, being among the best producers of these compounds in the plant world ( Clemente et al., 2011 ). One of them, digoxin, is mainly produced in Europe from dried leaves of D. lanata , reaching up to 1.5% dry weight ( Kennedy, 1978 ; Bown, 1995 ; Clemente et al., 2011 ).…”

Section: Introductionmentioning

confidence: 99%

“…are both widely collected from nature and field-cultivated to extract cardenolides, being among the best producers of these compounds in the plant world ( Clemente et al., 2011 ). One of them, digoxin, is mainly produced in Europe from dried leaves of D. lanata , reaching up to 1.5% dry weight ( Kennedy, 1978 ; Bown, 1995 ; Clemente et al., 2011 ). There is a vast diversity of cardenolides within the genus Digitalis with more than one hundred different compounds isolated, the most commercially attractive, besides digoxin, being lanatoside C, digitoxin, and acetyldigitoxin ( Clemente et al., 2011 ; Kreis, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

“…One of them, digoxin, is mainly produced in Europe from dried leaves of D. lanata , reaching up to 1.5% dry weight ( Kennedy, 1978 ; Bown, 1995 ; Clemente et al., 2011 ). There is a vast diversity of cardenolides within the genus Digitalis with more than one hundred different compounds isolated, the most commercially attractive, besides digoxin, being lanatoside C, digitoxin, and acetyldigitoxin ( Clemente et al., 2011 ; Kreis, 2017 ). Other compounds, such as digitanols, steroidal saponins, anthranoids, phenols, sterols, and polysaccharides, have been identified in various Digitalis species (see Clemente et al., 2011 and Kreis, 2017 for reviews).…”

Section: Introductionmentioning

confidence: 99%

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Variation in the chemical profiles of three foxglove species in the central Balkans

et al. 2023

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The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species (D. lanata, D. ferruginea, and D. grandiflora) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis (Plantaginaceae) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora. The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p-coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p-coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea. However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis.

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Biotechnological Approaches for Biomass and Cardenolide Production in Digitalis purpurea L.

Pérez-Alonso

Chong-Pérez

Capote

et al. 2016

Methods in Molecular Biology

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Digitalis purpurea L. is one of the main economically viable sources of cardenolides (cardiac glycosides) for the pharmaceutical industry. Nevertheless, production of cardenolides in plants grown by traditional agriculture is not always an efficient process and can be affected by biotic and abiotic factors. This chapter provides two biotechnology strategies for biomass and cardenolide production in D. purpurea. Firstly, we report biomass production using a temporary immersion system (TIS), combined with cardenolide extraction and quantification. Secondly, an efficient protocol for genetic transformation via Agrobacterium tumefaciens is provided. These strategies can be used independently or combined in order to increase the content of cardiac glycosides in D. purpurea and to unravel biosynthetic pathways associated to cardiac glycoside production.

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Digitalis

Cited by 9 publications

References 208 publications

Efﬁcient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.

Efﬁcient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.

Variation in the chemical profiles of three foxglove species in the central Balkans

Biotechnological Approaches for Biomass and Cardenolide Production in Digitalis purpurea L.

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