Efficient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.

Enhancement of cardenolide production in transgenic Digitalis purpurea L. by expressing a progesterone-5β-reductase from Arabidopsis thaliana L.

“…According to a recently published propagation protocol, D. purpurea plants grown in vitro using 6-BAP can contain around 220 μg/gDW of digitoxin and 22 μg/gDW of digoxin (Pérez-Alonso et al, 2018). In this study, similar values were observed in control plants, but higher cardenolide accumulation could be obtained in lines expressing the VEP1 gene.…”

“…The high variability observed for cardenolide content in greenhouse-grown plants as well as the difference in cardenolide accumulation between plants cultivated in vitro and in the greenhouse (Figure 7), may be explained by the less uniform growth conditions in the greenhouse. Indeed, it has been demonstrated that multiple factors can modify cardenolide concentration, like temperature, light, mineral soil composition, season, humidity, and others (Sales et al, 2011;Pérez-Alonso et al, 2018). Recently, cardenolide production in D. purpurea in a plant factory was enhanced under the influence of different LED exposure.…”

NaCl induced salt adaptive changes and enhanced accumulation of 20-hydroxyecdysone in the in vitro shoot cultures of Spinacia oleracea (L.)

“…The in vitro technique is more advantageous due to enhanced efficient and rapid isolation of secondary metabolites independent of ambient weather and microbial contamination 12 . It has been suggested that the plant tissue culture technique can assist the commercial scale production of secondary metabolites for a wide variety of pharmaceutical and industrial applications 13 . Although accumulation of bioactive 20E has been evidenced in different plant species, there is no report on the salinity induced 20E accumulation in Spinacia oleracea in vitro grown cultures.…”

Enhanced growth and cardenolides production in Digitalis purpurea under the influence of different LED exposures in the plant factory

“…Utilizing the mechanisms of plant tissue culture and plant factory techniques have become the need of the hour 3 for researches, that are being conducted on modern agricultural biotechnology and for large-scale production of cardiotonic glycoside and its derivatives 3 . Few reports till date are available regarding the cardenolides accumulation from in vitro propagation 4–6 and temporary immersion bioreactor 7 in D. purpurea , however the in vitro morphophysiological growth parameters and production of cardenolides in a plant factory system (PFS) have not been studied yet.…”