Digital holographic microscopy for microalgae biovolume assessment

Monaldi, Andrea Carolina; Romero, Gladis Graciela; Alanís, Elvio; Cabrera, Carla

doi:10.1016/j.optcom.2014.10.035

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…The utility of DHM for detection of microorganisms (especially in sparse environmental samples) has been demonstrated for numerous applications, such as diagnostics, water quality measurements, and ocean monitoring of microbial populations and behavior [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. The technique has also been used to investigate the basic biophysics of microbial motility [ 35 , 36 , 37 , 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

“…The image stacks can be analyzed for particle positions, and those positions tracked over time to obtain particle trajectories. The utility of DHM for detection of microorganisms (especially in sparse environmental samples) has been demonstrated for numerous applications, such as diagnostics, water quality measurements, and ocean monitoring of microbial populations and behavior [27][28][29][30][31][32][33][34]. The technique has also been used to investigate the basic biophysics of microbial motility [35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

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Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy

Rouzie

Lindensmith

Nadeau

2021

Life

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Digital holographic microscopy provides the ability to observe throughout a volume that is large compared to its resolution without the need to actively refocus to capture the entire volume. This enables simultaneous observations of large numbers of small objects within such a volume. We have constructed a microscope that can observe a volume of 0.4 µm × 0.4 µm × 1.0 µm with submicrometer resolution (in xy) and 2 µm resolution (in z) for observation of microorganisms and minerals in liquid environments on Earth and on potential planetary missions. Because environmental samples are likely to contain mixtures of inorganics and microorganisms of comparable sizes near the resolution limit of the instrument, discrimination between living and non-living objects may be difficult. The active motion of motile organisms can be used to readily distinguish them from non-motile objects (live or inorganic), but additional methods are required to distinguish non-motile organisms and inorganic objects that are of comparable size but different composition and structure. We demonstrate the use of passive motion to make this discrimination by evaluating diffusion and buoyancy characteristics of cells, styrene beads, alumina particles, and gas-filled vesicles of micron scale in the field of view.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy

Rouzie

Lindensmith

Nadeau

2021

Life

View full text Add to dashboard Cite

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“…These perturbations may vary in an unpredictable way from one acquisition to another because they depend on random external conditions, which are difficult to control. Since in many DHM applications accurate phase values must be extracted from the quantitative phase map [11], this aberration must be compensated in order to have access to reliable local information of the integrated optical path length (OPL) which can be used to measure either the integral refractive index or the topography of the object under study.…”

Section: Introductionmentioning

confidence: 99%