Digital gene expression profiling in larvae of Tribolium castaneum at different periods post UV-B exposure

Guo, Shu-Hao; Yu, Lin; Liu, Yanmei; Wang, Fei-Feng; Chen, Yu‐Chen; Wang, Ye; Qiu, Bao‐Li; Sang, Wen

doi:10.1016/j.ecoenv.2019.03.008

Cited by 12 publications

(7 citation statements)

References 72 publications

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“…Regarding detoxification and antioxidative mechanisms, glutathione S-transferase can catalyze the binding of the electrophilic group of endogenous harmful substances produced by UV-B stress to the thiol group of reduced glutathione, thereby forming a more soluble, nontoxic derivative that is easily excreted or decomposed by metabolic enzymes ( Coleman et al 1997 ). The activity of glutathione S-transferase in Helicoverpa armigera adults and T. castaneum were also significantly increased under UV stress ( Meng et al 2009 , Guo et al 2019 ). As an important serine hydrolase in insects, carboxylesterase can effectively catalyze the hydrolysis of various endogenous and exogenous compounds containing carboxyl ester bonds, amide bonds, and thioester bonds ( Karunaratne et al 1995 ).…”

Section: Discussionmentioning

confidence: 99%

“…UV can result in ROS accumulation in insect cells, and the imbalance between ROS production and antioxidation can directly lead to a variety of toxic effects, including nonspecific DNA, protein, and lipid damage ( Pitzschke et al 2006 , Meng et al 2009 , Gill and Tuteja 2010 , Wang el al. 2012 , Guo et al 2019 , Zhao et al 2019 ). Several antioxidant-related genes, fatty acyl-CoA reductase (Unigene15357 and Unigene11089), hydroxymethylglutaryl-CoA lyase (Unigene5738), mpv17-like protein 2 (Unigene7104), and long-chain-fatty-acid-CoA ligase were significantly upregulated after UV-B treatment.…”

Section: Discussionmentioning

confidence: 99%

“…High doses of UV-B radiation are harmful to living organisms. It induces oxidative stress in living organisms by generating reactive oxygen species, leading to the destruction of DNA, membrane lipids, and proteins, which seriously affects growth, physiology, biochemistry, and population structure ( Lidon et al 2012 , Hideg et al 2013 , Guo et al 2019 ).…”

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confidence: 99%

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Transcriptome Analysis of Myzus persicae to UV-B Stress

Yang

Meng

Yao

et al. 2021

Journal of Insect Science

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As an environmental stress factor, ultraviolet-B (UV-B) radiation directly affects the growth and development of Myzus persicae (Sulzer) (Homoptera: Aphididae). How M. persicae responds to UV-B stress and the molecular mechanisms underlying this adaptation remain unknown. Here, we analyzed transcriptome data for M. persicae following exposure to UV-B radiation for 30 min. We identified 758 significant differentially expressed genes (DEGs) following exposure to UV-B stress, including 423 upregulated and 335 downregulated genes. In addition, enrichment analysis using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases illustrated that these DEGs are associated with antioxidation and detoxification, metabolic and protein turnover, immune response, and stress signal transduction. Simultaneously, these DEGs are closely related to the adaptability to UV-B stress. Our research can raise awareness of the mechanisms of insect responses to UV-B stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Transcriptome Analysis of Myzus persicae to UV-B Stress

Yang

Meng

Yao

et al. 2021

Journal of Insect Science

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“…Previous research has shown that UV-C radiation upregulates the expression of stress-responsive genes such as heat shock protein 70 (hsp70), heat shock protein cognate 70 (hsc70), and heat shock protein 90 (hsp90) in the maize weevil Sitophilus zeamais Motschulsky (Tungjitwitayakul et al, 2016). Recently, a digital gene expression analysis showed that UV-B radiation was involved in the downregulation and upregulation of numerous genes (310-996 genes) in T. castaneum after 4 and 24 h of exposure (Guo et al, 2019). In this study, various significant abnormal characteristics were found on the adult antennae.…”

Section: Discussionmentioning

confidence: 99%

Impact of UV-C radiation on morphology of the antenna and antennal sensilla in Tribolium castaneum (Coleoptera: Tenebrionidae)

Tungjitwitayakul

Yasanga

Tatun

2020

Journal of Radiation Research and Applied Sciences

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The present study reveals the effects of ultraviolet-C on the morphology of the antenna and antennal sensilla of Tribolium castaneum. Pupae were irradiated with UV-C at a distance of 35 cm for 1, 2, 4, 8, 16, 32, or 64 min. The morphometric traits of the antenna and antennal sensilla were determined using light and scanning electron microscopy. UV-C caused abnormalities to the adults' antennae that were classified into 3 levels; mildly, moderately and strongly affected. A loss of segmentation occurred in the funicle and club, and the antennae were greatly reduced in size overall. The severity of the antennal abnormalities occurred in a dosedependent manner. The UV-C radiation significantly decreased the length of the scape, funicle, and club and the width of the club. Scanning electron microscopy of the resulting adults showed the antennae had a wrinkled cuticle, and the sensilla were fewer in number. The long sensilla trichodea became shorter, while the long and short sensilla trichodea were broader. Likewise, the width of sensilla basiconica subtypes 1 and 2 also increased after the UV-C irradiation. The results indicate that UV-C irradiation causes specific malformations in the antennae and antennal sensilla of T. castaneum.

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“…A few studies have reported the physiological responses of insect hosts to pathogen infection by analyzing host enzymatic activities and gene expression levels or by transcriptomic profiling [33,[38][39][40]. Such transcriptomic analysis is a widely used method to study the effects of environmental stressors, including UV exposure [41], insecticidal stress [40,42], and entomopathogenic stress [39], on different insect species. These studies have provided useful insights into the underlying mechanisms and interactions of hosts and pathogens or substances.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of the Brown Planthopper, Nilaparvata lugens, at Different Stages after Metarhizium anisopliae Challenge

Peng

Tang

Xie

2020

Insects

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Nilaparvata lugens is one of the major pests of rice and results in substantial yield loss every year. Our previous study found that the entomopathogenic fungus Metarhizium anisopliae showed effective potential for controlling this pest. However, the mechanisms underlying M. anisopliae infection of N. lugens are not well known. In the present study, we further examined the transcriptome of N. lugens at 4 h, 8 h, 16 h, and 24 h after M. anisopliae infection by Illumina deep sequencing. In total, 174.17 Gb of data was collected after sequencing, from which 23,398 unigenes were annotated by various databases, including 3694 newly annotated genes. The results showed that there were 246 vs 75, 275 vs 586, 378 vs 1055, and 638 vs 182 up- and downregulated differentially expressed genes (DEGs) at 4 h, 8 h, 16 h, and 24 h after M. anisopliae infection, respectively. The biological functions and associated metabolic processes of these genes were determined with the Clusters of Orthologous Groups (COG), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The DEGs data were verified using RT-qPCR. These results indicated that the DEGs during the initial fungal infection appropriately reflected the time course of the response to the fungal infection. Taken together, the results of this study provide new insights into the molecular mechanisms underlying the insect host response to fungal infection, especially during the initial stage of infection, and may improve the potential control strategies for N. lugens.

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Digital gene expression profiling in larvae of Tribolium castaneum at different periods post UV-B exposure

Cited by 12 publications

References 72 publications

Transcriptome Analysis of Myzus persicae to UV-B Stress

Transcriptome Analysis of Myzus persicae to UV-B Stress

Impact of UV-C radiation on morphology of the antenna and antennal sensilla in Tribolium castaneum (Coleoptera: Tenebrionidae)

Transcriptomic Analysis of the Brown Planthopper, Nilaparvata lugens, at Different Stages after Metarhizium anisopliae Challenge

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