Transcriptome Analysis of Myzus persicae to UV-B Stress

Abstract

As an environmental stress factor, ultraviolet-B (UV-B) radiation directly affects the growth and development of Myzus persicae (Sulzer) (Homoptera: Aphididae). How M. persicae responds to UV-B stress and the molecular mechanisms underlying this adaptation remain unknown. Here, we analyzed transcriptome data for M. persicae following exposure to UV-B radiation for 30 min. We identified 758 significant differentially expressed genes (DEGs) following exposure to UV-B stress, including 423 upregulated and 335 downregulated genes. In addition, enrichment analysis using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases illustrated that these DEGs are associated with antioxidation and detoxification, metabolic and protein turnover, immune response, and stress signal transduction. Simultaneously, these DEGs are closely related to the adaptability to UV-B stress. Our research can raise awareness of the mechanisms of insect responses to UV-B stress.

Mechanistic effects of microwave radiation on pupal emergence in the leafminer fly,Liriomyza trifolii

“…In Myzus persicae exposed to UV-A radiation, DEGs were associated with antioxidants and detoxification, metabolism and protein turnover, the immune response, and stress-related signal transduction; furthermore, the shd gene, which encodes 20-hydroxylase and converts ecdysone into 20E, was significantly down-regulated during UV-B exposure. The synthesis of 20E in M. persicae may be inhibited by UV-B, which needs to be confirmed by further experiments (Yang et al , 2021). In the present study, genes encoding cuticular protein in L. trifolii were significantly up-regulated in response to microwave radiation.…”

“…In response to UV-A radiation, comparative transcriptome analysis between UV-treated and control groups in O. furnacalis indicated the involvement of pathways associated with signal transduction, detoxification, the stress response, immune defense, and antioxidative systems (Su et al , 2021). Meanwhile, antiviral and FcγR-mediated phagocytosis of immune-related genes were induced during exposure to UV-B radiation, which indicates that insects have strong immune-adaptive functions (Adamo, 2012, 2017; Yang et al , 2021). In the current study, microwave radiation significantly reduced the expression of several immune-related genes, including serine protease inhibitor and serine/threonine-protein phosphatase.…”

“…Similarly, microwave radiation induced Hsp70 expression in chick embryos (Shallom et al , 2002) and human neuroblastoma cells (Calabrò et al , 2012). The effects of other nonionizing radiation treatments have been reported in insect pests, and genes encoding Hsps and antioxidant enzymes have been studied as mechanisms that provide some tolerance to radiation (Tungjitwitayakul et al , 2016; Su et al , 2021; Yang et al , 2021). In S. zeamais , Szhsp70 and Szhsp90 were induced after UV-C radiation, and Szhsp70 was expressed at a much higher level than Szhsp90 (Tungjitwitayakul et al , 2016).…”

“…Microwave radiation was shown to increase the expression of genes encoding heat shock protein (Hsps) in the maize weevil, Sitophilus zeamais (Tungjitwitayakul et al, 2016). Although we are unware of other studies on the mechanism of microwave radiation in insects, the effects of other nonionizing forms of radiation on insects indicated that genes encoding Hsps and antioxidant enzymes are involved (Tungjitwitayakul et al, 2016;Su et al, 2021;Yang et al, 2021). For instance, the response of the tephritid fruit fly, Bactrocera dorsalis, to UV radiation indicated that antioxidant enzyme activity was irreversibly reduced (Cui et al, 2021).…”

Comparative transcriptomics of the irradiated melon fly (Zeugodacus cucurbitae) reveal key developmental genes

“…We used the HiSeq 4000 PE Cluster Kit (Illumina) to cluster the index-coded samples on the Cluster Generation System following manufacturer instructions. The transcriptome was then sequenced using the Illumina Hiseq 4000 platform to generate 150 bp paired-end reads ( Yang et al, 2021 ). Initial processing of raw fastq data (raw reads) was performed using custom Perl scripts ( Ma et al, 2022 ).…”