Digital gene expression analysis of reproductive toxicity of benzo[a]pyrene in male scallop chlamys farreri

Deng, Xuxu; Pan, Luqing; Miao, Jingjing; Cai, Yuefeng; Hu, Fengxiao

doi:10.1016/j.ecoenv.2014.09.002

Cited by 24 publications

(4 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently, the NGS-based RNA-Seq approach has been widely employed for the identification and profiling of apoptosis-related genes in many species (Deng et al, 2014; Liu et al, 2015; Yue et al, 2015). In this study, using RNA-Seq technology, many DEGs involved in apoptosis regulation were identified and characterized at the different stages of spermatogenesis in P. sinensis .…”

Section: Discussionmentioning

confidence: 99%

Molecular and Cellular Mechanisms of Apoptosis during Dissociated Spermatogenesis

et al. 2017

View full text Add to dashboard Cite

Apoptosis is a tightly controlled process by which tissues eliminate unwanted cells. Spontaneous germ cell apoptosis in testis has been broadly investigated in mammals that have an associated spermatogenesis pattern. However, the mechanism of germ cell apoptosis in seasonally breeding reptiles following a dissociated spermatogenesis has remained enigmatic. In the present study, morphological evidence has clearly confirmed the dissociated spermatogenesis pattern in Pelodiscus sinensis. TUNEL and TEM analyses presented dynamic changes and ultrastructural characteristics of apoptotic germ cells during seasonal spermatogenesis, implying that apoptosis might be one of the key mechanisms to clear degraded germ cells. Furthermore, using RNA-Seq and digital gene expression (DGE) profiling, a large number of apoptosis-related differentially expressed genes (DEGs) at different phases of spermatogenesis were identified and characterized in the testis. DGE and RT-qPCR analysis revealed that the critical anti-apoptosis genes, such as Bcl-2, BAG1, and BAG5, showed up-regulated patterns during intermediate and late spermatogenesis. Moreover, the increases in mitochondrial transmembrane potential in July and October were detected by JC-1 staining. Notably, the low protein levels of pro-apoptotic cleaved caspase-3 and CytC in cytoplasm were detected by immunohistochemistry and western blot analyses, indicating that the CytC-Caspase model might be responsible for the effects of germ cell apoptosis on seasonal spermatogenesis. These results facilitate understanding the regulatory mechanisms of apoptosis during spermatogenesis and uncovering the biological process of the dissociated spermatogenesis system in reptiles.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular and Cellular Mechanisms of Apoptosis during Dissociated Spermatogenesis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Digital gene expression (DGE) with high-throughput sequencing technologies is a novel and efficient approach to genome-wide expression profiling using next generation sequencing methodologies. This approach allows researchers to identify, quantify, and annotate expression at the whole genome level with or without prior sequence knowledge of any transcript from any organism, and has been used in many studies (Deng et al, 2014;Zhang et al, 2014). Overall, DGE dramatically enhances the identification of differentially expressed genes.…”

Section: Introductionmentioning

confidence: 98%

Transcriptome profiling reveals the candidate genes associated with aroma metabolites and emission of pear (Pyrus ussuriensis cv.)

Wei

Tao

Qin

et al. 2016

Scientia Horticulturae

View full text Add to dashboard Cite

Transcriptome profiling reveals the candidate genes associated with aroma metabolites and emission of pear (Pyrus ussuriensis cv.) ଝ a b s t r a c t Aroma is an important factor affecting pear fruit quality. However, the molecular basis for the development of aroma metabolites and their emission is poorly understood. Unripe 'Nanguoli' pear fruit has poor aroma, while the ripe fruit has an intense aroma, and calcium treatment can enhance the aroma, providing good material for transcriptome analysis.Digital gene expression (DGE) analysis of 'Nanguoli' fruit was performed at three important developmental stages, with and without calcium treatment, six libraries in total. Of the total number of mapped reads (11.4-13.6 million), an average of 69.81% could be mapped to the pear genome. A total of 10,776 differentially expressed genes between the poor aroma and intense aroma were categorized into 36 functional groups and 37 pathways according to GO and KEGG databases, respectively. Genes more highly expressed in the more aromatic fruit include some from the fatty acid pathway such as FAD, ADH, PDC, LOX, and lipase genes. Some transcription factors were identified with high correlation to aroma biosynthesis in pear. Also, ABA may play an important role in the calcium-triggered improvement of 'Nanguoli' aroma. In addition, candidate genes were verified with quantitative real-time PCR.

show abstract

“…20 To date, transcriptome studies using RNA-seq have been reported for various marine bivalves such as the scallop Chlamys farreri, the blue mussel Mytilus galloprovincialis, the Manila clam Ruditapes philippinarum, and the pearl oyster Pinctada fucata. [21][22][23][24] Therefore, transcriptome data from these non-model animals have demonstrated much about their underlying toxic effects in response to different environmental stressors. 22,25 The green mussel, P. viridis, is one of the most important bivalves in tropical marine ecosystems.…”

Section: Introductionmentioning

confidence: 99%

Differential gene responses in the embryo of the green mussel Perna viridis exposed to dichlorodiphenyltrichloroethane (DDT)

et al. 2017

View full text Add to dashboard Cite

The green-lipped mussel, , is considered to be an ideal indicator for marine environmental pollution. Dichlorodiphenyltrichloroethane (DDT), a typical persistent organic pollutant, is extensively distributed in marine environments. However, little is known about the toxic effects of DDT on the embryo of marine animals, particularly in marine bivalves. Using next-generation sequencing technology, we studied embryo after DDT stress at the transcriptome level. A total of 99 202 unigenes were obtained based on the 2383 bp of unigene N50. These differentially expressed genes (DEGs) participated in the various molecular pathways of biological effects, including oxidative stress, detoxification, innate immunity and neurobehavioral disease. Quantitative real-time PCR was performed to verify the mRNA expression of several genes identified by differential gene expression (DGE) analysis. The results indicated that DDT was in induced a dose-dependent manner in the embryo of , and most genes involved in oxidative stress and detoxification were up-regulated by DDT exposure; however, the immunity-related genes were down-regulated, except the genes involved in phagocytosis. Gene expression changes in embryo from provide a preliminary basis to better understand the molecular toxic response mechanisms of embryo to DDT.

show abstract

Digital gene expression analysis of reproductive toxicity of benzo[a]pyrene in male scallop chlamys farreri

Cited by 24 publications

References 54 publications

Molecular and Cellular Mechanisms of Apoptosis during Dissociated Spermatogenesis

Molecular and Cellular Mechanisms of Apoptosis during Dissociated Spermatogenesis

Transcriptome profiling reveals the candidate genes associated with aroma metabolites and emission of pear (Pyrus ussuriensis cv.)

Differential gene responses in the embryo of the green mussel Perna viridis exposed to dichlorodiphenyltrichloroethane (DDT)

Contact Info

Product

Resources

About