Differential gene responses in the embryo of the green mussel Perna viridis exposed to dichlorodiphenyltrichloroethane (DDT)

Abstract

The green-lipped mussel, , is considered to be an ideal indicator for marine environmental pollution. Dichlorodiphenyltrichloroethane (DDT), a typical persistent organic pollutant, is extensively distributed in marine environments. However, little is known about the toxic effects of DDT on the embryo of marine animals, particularly in marine bivalves. Using next-generation sequencing technology, we studied embryo after DDT stress at the transcriptome level. A total of 99 202 unigenes were obtained based on the 2383 bp of unigene N50. These differentially expressed genes (DEGs) participated in the various molecular pathways of biological effects, including oxidative stress, detoxification, innate immunity and neurobehavioral disease. Quantitative real-time PCR was performed to verify the mRNA expression of several genes identified by differential gene expression (DGE) analysis. The results indicated that DDT was in induced a dose-dependent manner in the embryo of , and most genes involved in oxidative stress and detoxification were up-regulated by DDT exposure; however, the immunity-related genes were down-regulated, except the genes involved in phagocytosis. Gene expression changes in embryo from provide a preliminary basis to better understand the molecular toxic response mechanisms of embryo to DDT.

Transcriptomic and Histological Analysis of the Greentail Prawn (Metapenaeus bennettae) Following Light Crude Oil Exposure

“…For example, killifish collected from oil‐affected and ‐unaffected locations differed in CYP1A protein expression profiles in the tissues of adult and larval fish, with an up‐regulation of CYP1A protein in the gills, liver, intestine, and head kidney indicating the metabolism of PAHs corresponded with the arrival of the Deepwater Horizon spilled oil (Dubansky et al, 2013). Such studies have been pivotal in improving our understanding of physiological and molecular responses triggered by environmental changes and the pathways underpinning stress responses in nonmodel species (Gomiero et al, 2006; Hook et al, 2010; Jiang et al, 2017).…”

Quantification of Residual Organochlorine Pesticides in Medicinal Plant Raw Materials Containing Terpenoids

“…За время использования ХОП было распылено несколько миллионов тонн этих веществ и в настоящее время они являются одними из основных загрязнителей окружающей среды, что усугубляется их химической стойкостью, низкой растворимостью в воде, выраженной липофильностью, высокой степенью аккумуляции в живых организмах. Они обладают выраженной мутагенной и канцерогенной активностью, способны нарушать репродуктивные и эндокринные функции различных живых существ [4][5][6][7].…”