Digestive proteases of the lugworm (<i>Arenicola marina</i>) inhibited by Cu from contaminated sediments

Chen, Zhen; Mayer, Lawrence M.

doi:10.1002/etc.5620170313

Cited by 12 publications

(9 citation statements)

References 15 publications

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“…For example, low MW histidine-metal complexes enhance gut uptake of metal in some organisms (28)(29)(30). In addition, we found that gut amino acids appear responsible for detoxification of excess Cu to digestive proteases of deposit feeders (10). In addition, we found that gut amino acids appear responsible for detoxification of excess Cu to digestive proteases of deposit feeders (10).…”

Section: Discussionmentioning

confidence: 75%

Mechanisms of Cu Solubilization during Deposit Feeding

Chen

Mayer

1998

Environ. Sci. Technol.

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Large amounts of Cu in contaminated sediments can be dissolved by digestive fluids of deposit feeders during an in vitro digestion (Mayer et al. Environ. Sci. Technol. 1996, 30, 2641 and may subsequently become available to those organisms. The mechanisms of this digestive dissolution were investigated by comparing Cu release potentials of modified and unmodified gut fluids. Microwave treatments indicated that complexation rather than enzymatic action likely accounts for enhanced Cu solubilization by gut fluids rich in protein. Solutions of a common protein, bovine serum albumin, with similar AA concentrations mimicked digestive fluids in releasing sedimentary Cu. Chemical modification of histidine residues in gut fluids drastically reduced Cu release, suggesting their important role in Cu bioavailability. Using an ion-selective electrode, we found an abundance of gut ligand sites with conditional stability constants >10 10 , consistent with histidine complexation. Histidine concentrations among various molecular weight fractions of gut fluids can be predictive of the amount of Cu released during in vitro digestion. Complexation-enhanced metal remobilization by gut fluids thus has significant impact on metal availability during deposit feeding.

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Section: Discussionmentioning

confidence: 75%

Mechanisms of Cu Solubilization during Deposit Feeding

Chen

Mayer

1998

Environ. Sci. Technol.

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“…The observed reduction in Cd absorption by P. pugio (i.e., AE-Cd% < TAM-Cd%) exposed to a higher concentration of dietary Cd (i.e., A. franciscana exposed to 1 lM Cd) may be related to digestive physiology (e.g., saturation of metal-binding sites) and/or toxicity (e.g., inhibition of digestive enzymes) and requires further study (Chen & Mayer, 1998;Chen, Mayer, Weston, Bock, & Jumars, 2002;Reinfelder, Fisher, Luoma, Nichols, & Wang, 1998).…”

Section: Discussionmentioning

confidence: 97%

Bioenhancement of cadmium transfer along a multi-level food chain

Seebaugh

Goto

Wallace

2005

Marine Environmental Research

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“…For example, we have found in separate work that copper can inactivate digestive enzymes if released in concentrations sufficient to bind to enzymatically active sites on the enzyme proteins [28]. Our approach provides a measure of bioavailability of a contaminant, which can then be excreted, immobilized in tissue, or cause toxic effects.…”

Section: Bioavailabilitymentioning

confidence: 98%

BENZO[a]PYRENE AND ZINC SOLUBILIZATION BY DIGESTIVE FLUIDS OF BENTHIC INVERTEBRATES—A CROSS-PHYLETIC STUDY

Mayer¹,

Weston²,

Bock³

2001

Environ Toxicol Chem

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Contaminant bioavailability via digestive exposure was examined for 18 species of marine benthic invertebrates, using incubation of digestive fluids with sediments that were spiked with either radiolabeled benzo-[a]-pyrene (BaP) or zinc. Interphyletic trends in contaminant solubilization were compared with measures of digestive biochemistry, including enzyme activities, surfactancy, pH, and fluid phase organic carbon, amino acids, and lipids. Contaminant solubilization ranged from values equal to that of a seawater control to as much as an order of magnitude higher but were lower than those obtained with commonly used chemical extractants. Digestive fluids from echinoderms and a cnidarian tended to be relatively weak, those from polychaetes and echiurans were relatively strong, and those from taxa such as sipunculans and mollusks were intermediate. These trends correlated strongly with concentrations or activities of digestive biochemicals but not with pH. These correlations are consistent with previous work on mechanisms of digestive solubilization of polycyclic aromatic hydrocarbons (PAH) and metals, though strong covariance among digestive parameters does not allow this approach to be used for identification of specific mechanism(s).

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Digestive proteases of the lugworm (Arenicola marina) inhibited by Cu from contaminated sediments

Cited by 12 publications

References 15 publications

Mechanisms of Cu Solubilization during Deposit Feeding

Mechanisms of Cu Solubilization during Deposit Feeding

Bioenhancement of cadmium transfer along a multi-level food chain

BENZO[a]PYRENE AND ZINC SOLUBILIZATION BY DIGESTIVE FLUIDS OF BENTHIC INVERTEBRATES—A CROSS-PHYLETIC STUDY

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