Digestibility and β-carotene release from lipid nanodispersions depend on dispersed phase crystallinity and interfacial properties

Abstract

The influence of interfacial structure and lipid physical state on colloidal stability and digestibility of solid lipid nanoparticle dispersions (SLN) and canola oil-in-water emulsions (COE) stabilized with the non-ionic surfactants Poloxamer 188 (P188) and Tween 20 (T20) were examined and the release of encapsulated β-carotene (BC) under simulated gastrointestinal conditions determined. While the SLN and COE were all stable during exposure to gastric conditions (mean diameter ∼115 nm), more destabilization was observed for the COE than SLN during the duodenal phase. ζ-Potential measurements indicated rapid adsorption of bile salts (BS) and phospholipids (PL) to both solid and liquid interfaces, with greater surfactant displacement observed for the COE. Compared to the SLN, significantly more lipolysis and BC transfer to the aqueous phase was observed for both the COE-P188 and COE-T20 (p < 0.05). The properties of the colloidal structures present in the aqueous phase, which are important in terms of the uptake of lipolytic products and lipophilic bioactives, depended on non-ionic surfactant type, the extent of lipid digestion, as well as the presence of BS and PL.

Influence of Lipid Content in a Corn Oil Preparation on the Bioaccessibility of β‐Carotene: A Comparison of Low‐Fat and High‐Fat Samples

“…Previous studies have reported that mucin could be able to cause both bridging or depletion flocculation of lipid droplets (Vingerhoeds and others ; Silletti and others ). The PSD data (Figure B and C) and confocal fluorescence images (Figure ) suggested that many of the lipid droplets did not aggregate and remained relatively small, which was due to the strong steric repulsion generated by the hydrophilic head groups of the nonionic surfactant (Golding and others ; Nik and others ; Qian and others ). Only a fraction of the lipid droplets may have aggregated because of differences in droplet size (larger droplets are more prone to depletion flocculation than smaller ones) or polymer concentration (there may have been insufficient mucin present to promote bridging or depletion flocculation of all the droplets).…”

Tripalmitin–Sodium Dodecyl Sulfate Emulsion Droplet Liquid vs. Solid State Impacts in vitro Digestive Lipolysis

“…They observed a slower rate and lower extent of in vitro lipolysis for a 20 wt% oil‐in‐water 1 wt% sodium stearoyl lactate emulsion containing an effective solid‐fat content of 25% compared to a completely liquid emulsion in agreement with the in vivo results. Similarly, using an in vitro model of digestion, Nik, Langmaid, and Wright () reported significantly higher lipolysis for emulsion particles composed of canola oil than for solid lipid particles based on canola stearin.…”

Influence of methylcellulose on attributes of β-carotene fortified starch-based filled hydrogels: Optical, rheological, structural, digestibility, and bioaccessibility properties

“…Many of these nutraceuticals cannot simply be introduced into functional foods in their pure form because of physicochemical constraints, such as limited solubility, chemical instability, and poor bioavailability (Liang, Shoemaker, Yang, Zhong, & Huang, 2013;McClements, 2013;Reboul, 2013;Tang & Zhong-Gui, 2007). These challenges can often be overcome using food-grade colloidal delivery systems, such as emulsions, nanoemulsions, microemulsions, solid lipid nanoparticles, and filled hydrogels (Mun, Kim, Shin, & McClements, 2015;Nik, Langmaid, & Wright, 2012;Tokle, Lesmes, Decker, & McClements, 2012;Verrijssen et al, 2014;Zhang et al, 2015). Lipophilic nutraceuticals can be encapsulated within lipid droplets that can readily be dispersed in water, that can protect the bioactives from degradation, and that are digested in the gastrointestinal tract (GIT) to form mixed micelles that enhance bioaccessibility (McClements, 2013).…”