Diffuse optical imaging using spatially and temporally modulated light

O’Sullivan, Thomas D.; Cerussi, Albert E.; Cuccia, David J.; Tromberg, Bruce J.

doi:10.1117/1.jbo.17.7.071311

Cited by 223 publications

(218 citation statements)

References 120 publications

(131 reference statements)

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“…The mean free path between scattering events in tissue for near infrared (NIR) photons is between 20-40 mm [42]. Clearly photons will have scattered many times, while traversing tissue of thickness 40 mm, leading to path lengths of many tens of centimetres.…”

Section: Optimised Instrumentmentioning

confidence: 99%

High sensitivity non‐invasive detection of calcifications deep inside biological tissue using Transmission Raman Spectroscopy

Ghiţă

Matousek

Stone

2017

Journal of Biophotonics

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The aim of this research was to develop a novel approach to probe non-invasively the composition of inorganic chemicals buried deep in large volume biological samples. The method is based on advanced Transmission Raman Spectroscopy (TRS) permitting chemical specific detection within a large sampling volume. The approach could be beneficial to chemical identification of the breast calcifications detected during mammographic X-ray procedures. The chemical composition of a breast calcification reflects the pathology of the surrounding tissue, malignant or benign and potentially the grade of malignancy. However, this information is not available from mammography, leading to excisional biopsy and histopathological assessment for a definitive diagnosis. Here we present, for the first time, a design of a new high performance deep Raman instrument and demonstrate its capability to detect type II calcifications (calcium hydroxyapatite) at clinically relevant concentrations and depths of around 40 mm in phantom tissue. This is around double the penetration depth achieved with our previous instrument design and around two orders of magnitude higher than that possible when using conventional Raman spectroscopy.

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Section: Optimised Instrumentmentioning

confidence: 99%

High sensitivity non‐invasive detection of calcifications deep inside biological tissue using Transmission Raman Spectroscopy

Ghiţă

Matousek

Stone

2017

Journal of Biophotonics

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“…A widely-used method to non-invasively investigate scattering media, such as biological tissues [1,2], is to use a continuous-wave (CW) source within the 600-1100 nm range, and detect light re-emitted at some distance ρ from the injection point. Upon increasing ρ, deeper structures are probed.…”

Section: Introductionmentioning

confidence: 99%

Towards next-generation time-domain diffuse optics for extreme depth penetration and sensitivity

Mora

Contini

Arridge

et al. 2015

Biomed. Opt. Express

108

103

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Light is a powerful tool to non-invasively probe highly scattering media for clinical applications ranging from oncology to neurology, but also for molecular imaging, and quality assessment of food, wood and pharmaceuticals. Here we show that, for a paradigmatic case of diffuse optical imaging, ideal yet realistic time-domain systems yield more than 2-fold higher depth penetration and many decades higher contrast as compared to ideal continuous-wave systems, by adopting a dense source-detector distribution with picosecond time-gating. Towards this aim, we demonstrate the first building block made of a source-detector pair directly embedded into the probe based on a pulsed Vertical-Cavity Surface-Emitting Laser (VCSEL) to allow parallelization for dense coverage, a Silicon Photomultiplier (SiPM) to maximize light harvesting, and a Single-Photon Avalanche Diode (SPAD) to demonstrate the time-gating capability on the basic SiPM element. This paves the way to a dramatic advancement in terms of increased performances, new high impact applications, and availability of devices with orders of magnitude reduction in size and cost for widespread use, including quantitative wearable imaging. Opt. 19, 086012 (2014). 26. S. Carraresi, T. S. Shatir, F. Martelli, and G. Zaccanti, "Accuracy of a perturbation model to predict the effect of scattering and absorbing inhomogeneities on photon migration," Appl. Opt. 40, 4622-4632 (2001). 27. D. Contini, F. Martelli, and G. Zaccanti, "Photon migration through a turbid slab described by a model based on diffusion approximation. I. Theory," Appl. Opt. 36, 4587-4599 (1997). #230929Received 23

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“…The modification of DOS employing structured illumination can be efficiently used for mapping of biotissue absorption coefficient [2,12,13]. Variation of illumination pattern, in particular, changing its spatial frequency, potentially allows to control measurement volume.…”

Section: Introductionmentioning

confidence: 99%

“…Reconstruction of absorption spectra from these measurements allows to evaluate chromophore content within the studied tissue such as oxy-and deoxyhemoglobin, lipid and water as well as to retrieve hemoglobin oxygen saturation index [1,2]. DOS is a perspective tool for a broad range of medical diagnostics tasks including breast cancer diagnostics [4][5][6], human brain functional diagnostics [7], monitoring of tumor in laboratory animals in experimental oncology [8].…”

Section: Introductionmentioning

confidence: 99%

Probing depth in diffuse optical spectroscopy and structured illumination imaging: a Monte Carlo study

Loginova

Сергеева

Fiks

et al. 2017

JBPE

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Abstract. Diffuse optical spectroscopy (DOS) and its modification employing structured illumination are widely used in monitoring biotissue oxygenation. In such measurements it is important to know the probing volume for definite source-detector configuration; however, it cannot be measured directly. Monte Carlo simulations allow to trace the probing depth of each individual photon contributing to the signal, which provides a numerical solution for this problem. In this study we investigate distributions of photons over maximal depth reached in turbid media (probing depth) with optical parameters typical for cutaneous tissues at the wavelength of 600 nm. Different configurations of probing illumination are considered, such as collimated point source, one-dimension sinusoidal and rectangular patterns. For collimated point source and zero source-detector separation the number of collected photons monotonously decreases with the probing depth while a pronounced maximum in the distribution is manifested with the increase of source-detector separation. The position of this maximum shifts to higher depths with the decrease of µa. For one-direction sinusoidal and rectangular illumination patterns it is shown that when the photons are collected near the center of a bright stripe, the peak of the distribution remains close to the surface. When the photons are collected near the center of a dark stripe the peak shifts towards higher depths with the decrease in spatial duty cycle and spatial frequency of the illumination pattern. Employment of rectangular illumination pattern seems more efficient for DOS applications due to wider abilities for controlling probing depth.

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Diffuse optical imaging using spatially and temporally modulated light

Cited by 223 publications

References 120 publications

High sensitivity non‐invasive detection of calcifications deep inside biological tissue using Transmission Raman Spectroscopy

High sensitivity non‐invasive detection of calcifications deep inside biological tissue using Transmission Raman Spectroscopy

Towards next-generation time-domain diffuse optics for extreme depth penetration and sensitivity

Probing depth in diffuse optical spectroscopy and structured illumination imaging: a Monte Carlo study

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