Diffuse and Enteroaggregative Patterns of Adherence of Enteric Escherichia coli Isolated from Aboriginal Children from the Kimberley Region of Western Australia

Abstract

Escherichia coli from 138 fecal samples from aboriginal children, in whom no other enteric pathogen was isolated (including enterovirulent E. coli), were examined for HEp-2 cell adhesion. Twenty-five (36.8%) of 68 children with diarrhea and 32 (45.7%) of 70 without diarrhea had diffusely adherent isolates, which were thus not associated with diarrhea (P > .25). However, after age stratification, children > or = 18 months showed a significant association of diffusely adherent E. coli with diarrhea (P < or = .05). Enteroaggregative E. coli were isolated from 12 children with diarrhea (17.6%) and 15 without diarrhea (21.4%); thus, there was no association with diarrhea (P > or = .5). Sixteen children with diarrhea (23.5%) and 6 without diarrhea (8.6%) carried isolates that caused detachment of the HEp-2 cell monolayer from the glass coverslip when examined in the adhesion assay and were significantly associated with diarrhea (P < or = .05). These isolates, termed cell-detaching E. coli, were different from all recognized classes of enterovirulent E. coli.

Escherichia coli Pathotypes in Pakistan from Consecutive Floods in 2010 and 2011

“…[18][19][20][21][22] On the other hand typical EPEC, a wellrecognized cause of infantile diarrhea, 4 contributed much less during the current study compared with atypical EPEC in previous studies. 23,24 Similar results have been observed in Bangladesh where the majority of ETEC producing LT, followed by LT + ST and ST producing were predominantly associated with flood associated diarrheal cases 25 compared with previous studies from China, India, Mexico, Myanmar, and Pakistan where strains predominantly produced ST. 26 The association of EAEC with diarrhea has not been frequently reported, 27 however in our study EAEC is one of the most frequently isolated pathotype in sporadic cases, whereas EPEC is from flood affected cases. Furthermore, younger children ( 5 years of age) are more often affected by EAEC than older children in both the flood affected and non-flood affected areas (Table 4).…”

Etiologies of acute, persistent, and dysenteric diarrheas in adults in Bangui, Central African Republic, in relation to human immunodeficiency virus serostatus.

“…33 Six strains harbored a phenotype (cell-detaching E. coli) representing a putative new enteric virulent category of E. coli previously identified in Australia. 34 According to other studies, these observations point to the existence of different mechanisms by which EAggEC strains may cause disease exist. 29,33,35 These isolates are under investigation for their epidemiologic features.…”

“…The EAggEC have been recognized as a cause of persistent diarrhea in HIVinfected infants in countries that have common borders with the Central African Republic 31 and in several other countries. [32][33][34] In the Central African Republic, EAggEC strains were found only in HIVϩ immunodeficient adults who had no other enteric pathogen and should be considered an opportunistic agent whenever isolated from diarrhea stools of AIDS patients. Conversely, more caution seems indicated for interpreting the pathogenic role of E. coli with localized or diffuse adherence phenotype encountered in this study.…”

Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea

“…Cell-detaching E. coli (CDEC) strains cause detachment of HEp-2 cell monolayers from coverslips. CDEC were significantly associated with diarrhoea in Australian aboriginal children [16], although another study in children did not find an association [17]. The results of the latter study showed a correlation between cell detachment (CD) and AEhaemolysin production.…”