Difficulty to establish vegetative compatibility of Japanese isolates of Verticillium dahliae Kleb. using melanin-synthesis deficient mutants.

Nagao, Hideyuki; Wakatabe, Dai; Iijima, Tsutomu

doi:10.2323/jgam.40.277

Cited by 7 publications

(7 citation statements)

References 25 publications

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“…Isolates of tomato strain also showed vegetative compatibility when examined by melanin synthesis-deficient mutants (Nagao et al, 1994). Compatibility among the isolates of tomato strain was proved again by using different kinds of mutants.…”

Section: Discussionmentioning

confidence: 89%

“…In a previous study using melanin-deficient color mutants, complementary mutants could not be obtained from the isolates of non-tomato strain and compatibility testing was not possible between tomato-strain and nontomato strain (Nagao et al, 1994). In this study, we conducted complementation tests with 27 isolates in Japan.…”

Section: Discussionmentioning

confidence: 99%

“…Puhalla and Hummel (1983) later proposed 16 VCGs for a world-wide collection of V. dahliae isolates. In our preliminary study, it was not easy to obtain color mutants from Japanese isolates and recognition of VCGs was unsuccessful (Nagao et al, 1994). Methods using microsclerotial color mutants or auxotrophic mutants had certain inherent disadvantages (Joaquim and Rowe, 1990).…”

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confidence: 99%

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Vegetative compatibility groups of Japanese isolates of Verticillium dahliae

et al. 1997

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Japanese isolates of Verticillium dahliae were examined for vegetative compatibility relationships using nitrate-nonutilizing mutants. Four levels of vegetative compatibility were differentiated according to the degree of compatibility between the tester mutants of nit1 and NitM. Wild-type growth with a complementation line greater than 5 mm wide was defined as "strong reaction (-I-+)", i.e., compatible. Ten out of 15 isolates showed compatibility and were separated into three groups, provisionally designated as VCGJ1, VCGJ2, and VCGJ3, depending upon their reactions. This method was used to estimate genetic diversity within a local population of V. dahliae. Another 12 isolates from Gunma Pref. were paired with tester isolates of the three vegetative compatibility groups proposed, Eight Gunma isolates were assigned to VCGJ1 or VCGJ2. Two isolates were incompatible with all testers. The remaining 2 isolates were self-incompatible. Thus, 18 out of 27 Japanese isolates of V. dahliae were assigned to VCGs: 8 to VCGJ1,7 to VCGJ2, and 3 to VCGJ3. VCGJ1 was compatible with both VCGJ2 and VCGJ3, but VCGJ2 and VCGJ3 showed a weak reaction with each other. Japanese isolates of V. dahliae were thus demonstrated to form a VC group comprising three subgroups.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Vegetative compatibility groups of Japanese isolates of Verticillium dahliae

et al. 1997

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“…albo - atrum isolates (Karapapa et al 1997; Steventon et al 2002; Collins et al 2003). This almost diploid status might be the reason why numerous mutagenesis approaches have failed (Ingram 1968; Hastie 1973; Jackson and Heale 1985; Nagao et al 1994; Subbarao et al 1995; Zeise and von Tiedemann 2001). Most filamentous ascomycetes are haploid (Glass and Kuldau 1992).…”

Section: Introductionmentioning

confidence: 99%

Molecular diagnosis to discriminate pathogen and apathogen species of the hybrid Verticillium longisporum on the oilseed crop Brassica napus

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Braus‐Stromeyer

Timpner

et al. 2012

Appl Microbiol Biotechnol

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The cruciferous fungal pathogen Verticillium longisporum represents an allodiploid hybrid with long spores and almost double the amount of nuclear DNA compared to other Verticillium species. V. longisporum evolved at least three times by hybridization. In Europe, virulent A1xD1 and avirulent A1xD3 hybrids were isolated from the oilseed crop Brassica napus. Parental A1 or D1 species are yet unknown whereas the D3 lineage represents Verticillium dahliae. Eleven V. longisporum isolates from Europe or California corresponding to hybrids A1xD1 or A1xD3 were compared. A single characteristic type of nuclear ribosomal DNA could be assigned to each hybrid lineage. The two avirulent A1xD3 isolates carried exclusively D3 ribosomal DNA (rDNA) which corresponds to V. dahliae. The rDNA of all nine A1xD1 isolates is identical but distinct from D3 and presumably originates from A1. Both hybrid lineages carry two distinct isogene pairs of four conserved regulatory genes corresponding to either A1 or D1/D3. D1 and D3 paralogues differ in several single nucleotide polymorphisms. Southern hybridization patterns confirmed differences between the A1 and D1/D3 isogenes and resulted in similar patterns for D1 and D3. Distinct signatures of the Verticillium transcription activator (VTA)2 regulatory isogene pair allow identification of V. longisporum hybrids by a single polymerase chain reaction and the separation from haploid species as V. dahliae or Verticillium albo-atrum. The combination between VTA2 signature and rDNA type identification represents an attractive diagnostic tool to discriminate allodiploid from haploid Verticillia and to distinguish between A1xD1 and A1xD3 hybrids which differ in their virulence towards B. napus.Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-012-4530-1) contains supplementary material, which is available to authorized users.

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“…In contrast, in a V. longisporum strain maintained in culture for 51 years, no gene loss was observed (Inderbitzin et al, 2011b). The amphidiploid nature of V. longisporum also explains why auxotrophic mutants for the study of vegetative compatibility groups could not be generated (Bhat and Subbarao, 1999;Joaquim and Rowe, 1990;Puhalla, 1979;Nagao et al, 1994;Subbarao et al, 1995;Zeise and Tiedemann, 2001).…”

Section: Evolutionary History Genomics and Pathogenicitymentioning

confidence: 99%