Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation

Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model

“…HITx was performed under general anesthesia (isofluorane). The surgical technique was like those previously described 3 ( Materials and Methods, SDC, http://links.lww.com/TXD/A52 ).…”

“… 2 By far, rat ITx models has been the most extensively used mainly as consequence of technical aspects and the availability of different strains. 3 Since the standardization of procedures in the last decade of the XXth. century, orthotopic ITx and heterotopic ITx (HITx) variants of ITx are used, each of them allowing the evaluation of different aspects of the process.…”

Modified Multivisceral Transplantation with Native Spleen Removal in Rats

“…Due to the limited evidence in experimental models of MMVTx in rats, 7 we have used our previous experience in rodents experimental surgery and the extensive literature published in isolated small bowel and multivisceral transplantation to transfer to our heterotopic MMVTx model as well as pre-and post-surgical animal handling, anesthetics, analgesics, and antibiotics, with a very good effectiveness. 8,9,[15][16][17][18][19][20] Focusing on vascular anastomosis, we performed an end-to-side arterial anastomosis between the donor and recipient aorta following the technique reported by Galvao et al In contrast, due to the heterotopic graft position, we performed a hand-to-sewn venous anastomosis between donor PV and recipient cava vein instead of the described cuff technique. 7 In agreement with the multicenter work published by our surgeons group in isolated intestinal transplantation in rats, 8 main complications observed during recipient MMVTx surgery were related to the arterial and venous anastomosis confection.…”

“…In the same way, terminal ileostomy was performed following reported techniques. 9,18,19 Other important contribution in the MMVTx model is the inclusion of the spleen in the graft and the native spleen removal. In the clinic, the spleen is no longer included in the graft because the risk of GVHD is increased.…”

Small bowel transplantation in rats, A multicenter experience summarizing the pitfalls to be overcome

“…This is a retrospective study performed by 4 active microsurgical research laboratories (Multiorgan Transplant Institute, Argentina; Organ and Tissue Transplant Laboratory, Argentina; La Paz University Hospital, Spain and Biomedical Research Center from Aragón, Spain) with extensive experience in experimental microsurgery and microsurgical SBTx techniques [10,15,[16][17][18][19][20].…”