Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model

Stringa, Pablo; Romanin, David Emmanuel; Lausada, Natalia; Gobbi, Rodrigo Papa; Zanuzzi, Carolina Natalia; Martín, Pedro; Abate, Juan Cruz; Cabanne, Ana; Arnal, Nathalie; Vecchio, Leandro; Milesi, Verónica; Portiansky, Enrique Leo; Gondolesi, Gabriel; Rumbo, Martı́n

doi:10.1097/txd.0000000000000718

Cited by 12 publications

(16 citation statements)

References 41 publications

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“…Samples of transplanted intestine were taken through intestinal ostomy at 0, 30 min, 48 h and 7 days after ITx. Histological and morphometric graft evaluation, Immunofluorescence staining, gene expression analysis and graft functional evaluation was performed ( 21 ) ( Supplementary Datas S3–S7 ).…”

Section: Methodsmentioning

confidence: 99%

Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation

Stringa¹,

Dezillio²,

Talayero

et al. 2023

Transpl Int

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There is an urgent need to address the shortage of potential multivisceral grafts in order to reduce the average time in waiting list. Since donation after circulatory death (DCD) has been successfully employed for other solid organs, a thorough evaluation of the use of intestinal grafts from DCD is warranted. Here, we have generated a model of Maastricht III DCD in rodents, focusing on the viability of intestinal and multivisceral grafts at five (DCD5) and twenty (DCD20) minutes of cardiac arrest compared to living and brain death donors. DCD groups exhibited time-dependent damage. DCD20 generated substantial intestinal mucosal injury and decreased number of Goblet cells whereas grafts from DCD5 closely resemble those of brain death and living donors groups in terms intestinal morphology, expression of tight junction proteins and number of Paneth and Globet cells. Upon transplantation, intestines from DCD5 showed increased ischemia/reperfusion damage compared to living donor grafts, however mucosal integrity was recovered 48 h after transplantation. No differences in terms of graft rejection, gene expression and absorptive function between DCD5 and living donor were observed at 7 post-transplant days. Collectively, our results highlight DCD as a possible strategy to increase multivisceral donation and transplantation procedures.

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Section: Methodsmentioning

confidence: 99%

Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation

Stringa¹,

Dezillio²,

Talayero

et al. 2023

Transpl Int

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“…Counting of apoptotic cells was performed considering the number of apoptotic cells in 10 fields per sample (including 5 photographs per sample) using a FV1000 confocal microscope (Olympus, Tokyo, Japan). (28)…”

Section: Evaluation Of Graft Damage and Cell Injury In The Living Modelmentioning

confidence: 99%

The Novel N,N‐bis‐2‐Hydroxyethyl‐2‐Aminoethanesulfonic Acid–Gluconate–Polyethylene Glycol–Hypothermic Machine Perfusion Solution Improves Static Cold Storage and Reduces Ischemia/Reperfusion Injury in Rat Liver Transplant

et al. 2019

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Organ transplantation is the treatment of choice against terminal and irreversible organ failure. Optimal preservation of the graft is crucial to counteract cold ischemia effects. As we developed an N,N‐bis‐2‐hydroxyethyl‐2‐aminoethanesulfonic acid–gluconate–polyethylene glycol (BGP)–based solution (hypothermic machine perfusion [HMP]), we aimed to analyze the use of this solution on static cold storage (SCS) of rat livers for transplantation as compared with the histidine tryptophan ketoglutarate (HTK) preservation solution. Livers procured from adult male Sprague Dawley rats were preserved with BGP‐HMP or HTK solutions. Liver total water content and metabolites were measured during the SCS at 0°C for 24 hours. The function and viability of the preserved rat livers were first assessed ex vivo after rewarming (90 minutes at 37°C) and in vivo using the experimental model of reduced‐size heterotopic liver transplantation. After SCS, the water and glycogen content in both groups remained unchanged as well as the tissue glutathione concentration. In the ex vivo studies, livers preserved with the BGP‐HMP solution were hemodynamically more efficient and the O2 consumption rate was higher than in livers from the HTK group. Bile production and glycogen content after 90 minutes of normothermic reperfusion was diminished in both groups compared with the control group. Cellular integrity of the BGP‐HMP group was better, and the histological damage was reversible. In the in vivo model, HTK‐preserved livers showed a greater degree of histological injury and higher apoptosis compared with the BGP‐HMP group. In conclusion, our results suggest a better role of the BGP‐HMP solution compared with HTK in preventing ischemia/reperfusion injury in the rat liver model.

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“…Adult male Sprague-Dawley rats (250 ± 30 g) were anaesthetized with Isofluorane (5 and 2 % for induction and maintenance, respectively). The small bowel (jejunum and ileum) with the vascular pedicle (superior mesenteric artery and portal vein) was isolated following a microsurgical procedure previously described (48).…”

Section: Mean Mesenteric Arterial Bed Pressure (Mabp)mentioning

confidence: 99%

“…RBCs are continuously perfused through a rat mesenteric vascular bed. This is a heterologous organ system where the entire vascular pedicle and the small intestine remain intact (57). Mean mesenteric arterial bed pressure (MABP, estimated as pressure in mmHg) was continuously recorded, and intravascular levels of ATP (ATPiv) and free Hb (to estimate lytic ATP release) were measured at discrete times in perfusate samples that leave the arterial bed through the portal vein.…”

Section: Accepted Manuscript 19mentioning

confidence: 99%

Regulation of extracellular ATP of human erythrocytes treated with α-hemolysin. Effects of cell volume, morphology, rheology and hemolysis

Denis

Lefevre

Álvarez

et al. 2019

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Gut Permeability and Glucose Absorption Are Affected at Early Stages of Graft Rejection in a Small Bowel Transplant Rat Model

Abstract: Supplemental digital content is available in the text.

Cited by 12 publications

References 41 publications

Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation

Experimental Assessment of Intestinal Damage in Controlled Donation After Circulatory Death for Visceral Transplantation

The Novel N,N‐bis‐2‐Hydroxyethyl‐2‐Aminoethanesulfonic Acid–Gluconate–Polyethylene Glycol–Hypothermic Machine Perfusion Solution Improves Static Cold Storage and Reduces Ischemia/Reperfusion Injury in Rat Liver Transplant

Regulation of extracellular ATP of human erythrocytes treated with α-hemolysin. Effects of cell volume, morphology, rheology and hemolysis

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