Differentiation of Streptococcus pneumoniae from Nonpneumococcal Streptococci of the Streptococcus mitis Group by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Werno, Anja M.; Martin, Coralie; Anderson, Trevor P.; Murdoch, David R.

doi:10.1128/jcm.00508-12

Cited by 85 publications

(64 citation statements)

References 26 publications

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“…and Anaerococcus spp.). However, while MALDI-TOF MS has a reported reliability of >95% (of routine isolates grown on solid media in the laboratory), 19 its limitations include inability to differentiate genetically similar organisms, such as Streptococcus pneumoniae from other members of the Streptococcus mitis group, 20 and Shigella species from Eschericheria coli. 21 Despite this, MALDI-TOF MS analysis can reduce manual workload for medical scientists and improve throughput of laboratory samples.…”

Section: Can Laboratory Results Be Expedited?mentioning

confidence: 99%

A commentary on the role of molecular technology and automation in clinical diagnostics

et al. 2014

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Section: Can Laboratory Results Be Expedited?mentioning

confidence: 99%

A commentary on the role of molecular technology and automation in clinical diagnostics

et al. 2014

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“…1,7,18,21,22 For instance, it has recently been reported that improvements to the database enable a more reliable distinction between S. pneumoniae and viridans group streptococci. 23,24 Because the reference databases are not static and expanded regularly to fill in current gaps in identification, updates will continue to improve the performance of MALDI-TOF MS. 18 To the best of our knowledge, this study represents the first implementation and use of MALDI-TOF MS for the identification of bacteria and fungi in a hospital in tropical Africa. MALDI-TOF MS has previously been implemented and used for research purposes in South Africa for protein identification and bacterial identification, but its use was restricted to a few bacterial species in environmental studies of plant pathology or river water.…”

Section: Discussionmentioning

confidence: 99%

The Ongoing Revolution of MALDI-TOF Mass Spectrometry for Microbiology Reaches Tropical Africa

Fall¹,

Lo²,

Samb-Ba³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) represents a revolution in routine pathogen identification in clinical microbiology laboratories. A MALDI-TOF MS was introduced to tropical Africa in the clinical microbiology laboratory of the Hô pital Principal de Dakar (Senegal) and used for routine pathogen identification. Using MS, 2,429 bacteria and fungi isolated from patients were directly assayed, leading to the identification of 2,082 bacteria (85.7%) and 206 fungi (8.5%) at the species level, 109 bacteria (4.5%) at the genus level, and 16 bacteria (0.75%) at the family level. Sixteen isolates remained unidentified (0.75%). Escherichia coli was the most prevalent species (25.8%) followed by Klebsiella pneumoniae (14.8%), Streptococcus agalactiae (6.2%), Acinetobacter baumannii (6.1%), Pseudomonas aeruginosa (5.9%), and Staphylococcus aureus (5.9%). MALDI-TOF MS has also enabled the detection of rare bacteria and fungi. MALDI-TOF MS is a powerful tool for the identification of bacterial and fungal species involved in infectious diseases in tropical Africa.

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“…93 One of MALDI-TOF MS drawbacks is that it exhibits reduced sensitivity for Gram-positive bacteria and polymicrobial infections with respect to conventional approaches; for example, it is not able to identify different species of the S. viridans group. 94 Furthermore, this proteomic technology cannot be applied directly to whole blood; a subculture is necessary. …”

Section: Maldi-tof Msmentioning

confidence: 99%

In vitro diagnosis of sepsis: a review

Marcello

Tumolo²,

Donno³

et al. 2016

PLMI

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Sepsis, severe sepsis and septic shock, systemic inflammatory response, and other related manifestations represent a relevant medical problem with high morbidity and mortality, despite the improvements in diagnosis, treatment, and preventive measures over the last few decades. The limited knowledge of the pathophysiology in association with the lack of in vitro diagnostic methods for the certain and quick determination of the causative microbiological agents and their antibiotic resistance means the condition is still critical and of high impact in health care. The current gold standard method to detect the sepsis-causing pathogens, which is based on blood culture, is still insufficiently sensitive and slow. The new culture-independent molecular biology-based techniques can lead to the identification of a broad range of microorganisms and resistance markers within a few hours and with high sensitivity and specificity; nevertheless, limitations of, for example, the polymerase chain reaction-based methods still hamper their application in the clinical routine. This review summarizes the in vitro diagnostic methods and their approach in the clinical diagnosis of the bloodstream infections, and explores their advantages and disadvantages at the current state of the art. A quick analysis of the future prospective in multiplex technologies for microbiological diagnosis of sepsis is also provided.

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Differentiation of Streptococcus pneumoniae from Nonpneumococcal Streptococci of the Streptococcus mitis Group by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Cited by 85 publications

References 26 publications

A commentary on the role of molecular technology and automation in clinical diagnostics

A commentary on the role of molecular technology and automation in clinical diagnostics

The Ongoing Revolution of MALDI-TOF Mass Spectrometry for Microbiology Reaches Tropical Africa

In vitro diagnosis of sepsis: a review

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