Differentiation of Setaria digitata and Setaria labiatopapillosa using Molecular Markers

Jayasinghe, D.R; Wijesundera, W.S.S.

doi:10.1016/s1090-0233(02)00157-0

Cited by 13 publications

(6 citation statements)

References 12 publications

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“…By means of random polymorphic DNA (RAPD)-PCR analyses, 107 bands have been identified, of which 86 were polymorphic. The polymorphic loci identified by the RAPD-PCR analyses can be studied further to develop species-specific probes/PCR primers for the identification of each species (Jayasinghe & Wijesundera, 2003).…”

Section: Nucleic Acid Metabolismmentioning

confidence: 99%

Biochemical composition and metabolic pathways of filarial worms Setaria cervi: search for new antifilarial agents

Ahmad

Srivastava

2007

J. Helminthol.

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The main problem regarding the chemotherapy of filariasis is that no safe and effective drug is available yet to combat the adult human filarial worms. Setaria cervi, the causal organism of setariasis and lumbar paralysis in cattle, is routinely employed as a model organism for conducting biochemical and enzymatic studies on filarial parasites. In view of the practical difficulties in procuring human strains of Wuchereria bancrofti and Brugia malayi for drug screening, the bovine filarial parasite S. cervi, resembling the human species in having microfilarial periodicity and chemotherapeutic response to known antifilarial agents, is widely used as a model in such studies. For a rational approach to antifilarial chemotherapy, knowledge of the biochemical composition and metabolic pathways of this helminth parasite may be of paramount importance, so that more potent antifilarial agents based on specific drug targets can be identified in drug discovery programmes. The present review provides an update on the biochemistry of the important metabolic pathways functioning within this potentially important bovine parasite, that have so far been studied, and on those that need to be investigated further so as to identify novel drug targets that can be exploited for designing new antifilarial drugs.

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Section: Nucleic Acid Metabolismmentioning

confidence: 99%

Biochemical composition and metabolic pathways of filarial worms Setaria cervi: search for new antifilarial agents

Ahmad

Srivastava

2007

J. Helminthol.

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“…Setaria infection is usually non-pathogenic but may cause mild chronic peritonitis in mammalian hosts (Anderson, 2000). The number of nematodes in individual host animals is usually low; however, there are known cases of epidemic disease causing serofibrinous peritonitis with severe morbidity and mortality in reindeer and moose (Jayasinghe and Wijesundera, 2003; Enemark et al ., 2017). Moreover, individuals of several Setaria species are capable of entering the central nervous system and migrating through the brain and spinal cord.…”

Section: Introductionmentioning

confidence: 99%

Setaria cervi(Filarioidea, Onchocercidae) undressing in ungulates: altered morphology of developmental stages, their molecular detection and complete sequencecox1 gene

et al. 2021

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This work introduces new morphological and molecular information on the filaroid nematode Setaria cervi (Rudolphi, 1819) obtained from 13 infected game ungulates out of 96 dissected. The hosts comprised the following: a single moose (Alces alces), ten red deer (Cervus elaphus) and two sika deer (Cervus nippon) originating from the western and northern regions of the Czech Republic. Based on the complete sequences of the gene encoding mitochondrial cytochrome c oxidase subunit 1 (cox1), all 20 females and four males belonged to the species S. cervi. We detected three developmental female stages (adult fertile females, juvenile L5 females and L4 female larvae) differing in size and some morphological traits as the subtle structure of peribuccal crown and shape and features of tail knob. Such differences were described in detail for the first time. The phylogenetic relationships within the family Onchocercidae have been evaluated using new information on the cox1 sequence of S. cervi (maximum likelihood method, GTR + I + G model). In accordance with the latest phylogenetic studies, the present analysis confirmed the ancient separation of the subclass Setariinae from the remaining two onchocercid lineages Dirofilariinae and Onchocerinae.

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“…There are two setaria species infecting cattle and buffaloes, viz. S. digitata and S. labiato-papillosa, the most common being S. digitata (Shoho 1958) which commonly dwells in peritoneal cavities without any apparent pathogenicity but in heavy infections it causes mild fibrinous peritonitis (Jayasinghe and Wijesundera 2003). The microfilariae (L 1 ) found in the blood stream of host causes microfilariosis in bovine natural hosts (Sigraskar et al 1999) and infectious form causes cerebrospinal nematodosis in unnatural hosts i.e., sheep, goat and horses (Rhee et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural studies on Setaria digitata by scaning electron microscopy

Kumar

2015

J Parasit Dis

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Ultrastructural details of adult male and female worms of Setaria species collected from the peritoneal cavities of bovines slaughtered in and around Hyderabad, Telangana, India were studied using scanning electron microscopy. The worms exhibited peribuccal crown with round mouth opening encircled by a pair of lateral appendages and slightly notched dorso and ventro projections at the anterior end where as bosses and fine cuticular striations were seen all over the body. Posterior end of female worm ended in a round knob with a pair of lateral appendages whereas three pairs of precloacal, a pair of adcloacal, three pairs of postcloacal papillae, a central papillae just in front of the cloaca, a single tongue shaped spicule emerging from the cloaca, and a phasmid in front of a pair of lateral appendages were seen at the posterior end of male worm. The ultrastructural details confirmed the worms as Seteria digitata.

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Differentiation of Setaria digitata and Setaria labiatopapillosa using Molecular Markers

Cited by 13 publications

References 12 publications

Biochemical composition and metabolic pathways of filarial worms Setaria cervi: search for new antifilarial agents

Biochemical composition and metabolic pathways of filarial worms Setaria cervi: search for new antifilarial agents

Setaria cervi(Filarioidea, Onchocercidae) undressing in ungulates: altered morphology of developmental stages, their molecular detection and complete sequencecox1 gene

Ultrastructural studies on Setaria digitata by scaning electron microscopy

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