Differentiation of Porcine TLR4 Gene Expression in Piglets of Different Ages

Pan, ZX; Chen, Z.; Liu, L.; Cao, Yi-Bin; Xie, Kaihui; Yang, Jie; Huang, Xing; Wu, Shuqing; Bao, Wang

doi:10.3923/javaa.2011.2312.2316

Cited by 4 publications

(3 citation statements)

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“…However, depending on the mucosal site some age effects were observed for each TLR, in particular for TLR2 in the cardiac-to-oxyntic transition region and for TLR2 and 4 in the oxyntic region. The present study confirmed preliminary data previously reported regarding TLRs expression in the stomach [ 24 , 25 ] and extended them to three different gastric locations using greater numbers of individuals. Moreover, age effects on TLRs expression have been investigated in pigs, but over a different time scale (at 1 day, 2 months and 5 months of age [ 24 ]; or pre-weaning [ 25 ]), and only TLR3 gene expression was found greater in adult pigs compared to younger pigs [ 24 ].…”

Section: Discussionsupporting

confidence: 91%

“…The present study confirmed preliminary data previously reported regarding TLRs expression in the stomach [ 24 , 25 ] and extended them to three different gastric locations using greater numbers of individuals. Moreover, age effects on TLRs expression have been investigated in pigs, but over a different time scale (at 1 day, 2 months and 5 months of age [ 24 ]; or pre-weaning [ 25 ]), and only TLR3 gene expression was found greater in adult pigs compared to younger pigs [ 24 ]. Toll-like receptor-3 (TLR3), which is activated by double-stranded RNA and plays a role in anti-viral immunity, was reported to be expressed to greater levels in the porcine stomach than in the intestine [ 24 , 26 ].…”

Section: Discussionsupporting

confidence: 91%

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Age-Related Expression of the Polymeric Immunoglobulin Receptor (pIgR) in the Gastric Mucosa of Young Pigs

et al. 2013

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To date few studies have addressed the development and function of the porcine gastric mucosal immune system and this is a major limitation to understanding the immunopathogenesis of infections occurring in young pigs. The polymeric immunoglobulin receptor (pIgR) mediates the transport of secretory immunoglobulins until luminal surface of the gut mucosa and the aim of this study was to investigate the time course of pIgR expression and to determine its localization in three functionally different porcine gastric sites during the suckling period and after weaning. An additional goal was to investigate the time course expression of toll-like receptors (TLRs) in relation to pIgR expression. Gastric samples were collected from the cardiac-to-oxyntic transition (Cd), the oxyntic (Ox), and the pyloric (Py) regions in 84 pigs, slaughtered before weaning (14, 21 and 28 days of age; 23, 23 and 19 pigs, respectively) and 14 days post-weaning (42 days of age, 23 pigs). PIgR was expressed in the mucosa of all the three gastric sites, and its transcript levels were modulated during suckling and after weaning, with regional differences. PIgR expression increased linearly during suckling (P=0.019) and also increased post-weaning (P=0.001) in Cd, it increased post-weaning in Py (P=0.049) and increased linearly during suckling in Ox (P=0.036). TLRs expression was also modulated during development: in Cd, TLR2 increased linearly during suckling (P=0.003); in Ox, TLR2 decreased after weaning (P=0.038) while TLR4 increased linearly during suckling(P=0.008). The expression of TLR2, 3 and 4 in Ox was positively correlated with pIgR expression (P<0.001).Importantly, both pIgR protein and mRNA were localized, by immunohistochemistry and in situ hybridization, respectively, in the gastric glands of the lamina propria. These results indicate that pIgR is actively synthesized in the gastric mucosa and suggest that pIgR could play a crucial role in gastric mucosal immune defense of growing pigs.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Age-Related Expression of the Polymeric Immunoglobulin Receptor (pIgR) in the Gastric Mucosa of Young Pigs

et al. 2013

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“…Our results revealed that the expression of TLR4 in the small intestine of piglets was significantly affected at 1 day post-weaning. However, Pan et al (2011) [29] reported that intestinal TLR4 expression was not affected in piglets at 8, 18, 30, or 35 days of age. The differences in the results could be attributed to the fact that the authors did not evaluate the effects of weaning on TLR4 expression.…”

Section: Discussionmentioning

confidence: 96%