Differentiation of pathogenic Escherichia coli strains in Brazilian children by PCR

Tornieporth, Nadia; John, James; Salgado, Katia; Jesus, Paulo de; Latham, Elizabeth; Melo, Maria Clotildes Nunes de; Gunzburg, Stuart; Riley, Lee W.

doi:10.1128/jcm.33.5.1371-1374.1995

Cited by 99 publications

(33 citation statements)

References 24 publications

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“…The small number of ETEC strains identified did not allow much chance for vaccine efficacy calculations. Nevertheless, ETEC has been reported to be a common diarrheal pathogen in Brazil (28). In the present study, although the number of TD episodes was higher after a visit to South America than after earlier visits to other places, EPEC rather than ETEC was the major pathogroup of E. coli found in these diarrheal episodes.…”

Section: Discussioncontrasting

confidence: 56%

Prevalence of Diarrheagenic Escherichia coli in Finns with or without Diarrhea during a Round-the-World Trip

Keskimäki

Mattila

Peltola³

et al. 2000

J Clin Microbiol

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The incidence of diarrhea and the prevalence of bacterial enteropathogens, viruses, and parasites in feces of subjects with and without diarrhea were evaluated in 204 Finns traveling round the world (from Finland to China, Malaysia, Australia, Fiji, Chile, and Brazil and back to Finland). Special emphasis was placed on the finding of diarrheagenic Escherichia coli (enterotoxigenic, enteropathogenic, Shiga toxin-producing, and enteroaggregative strains) by PCR from growth on primary culture plates. From the PCR-positive samples, corresponding strains were isolated, confirmed as E. coli, and O serotyped. Of all the subjects, 37% experienced a total of 90 episodes of diarrhea. No adenoviruses or rotaviruses were detected, and findings of parasites were insignificant. In contrast, enteropathogenic bacteria were present in 62% of the 65 diarrheal and in 33% of the 127 nondiarrheal samples (P < 0.001); diarrheagenic E. coli strains were found in 35 and 26% of these, respectively (not statistically significant). As a single pathogen, E. coli was found in 20 and 24% of samples (not significant). Of all diarrheagenic E. coli strains, enteropathogenic strains were the most commonly found independently of the clinical picture of the subjects, whereas Salmonella enterica as a single pathogen was the most common non-E. coli organism found in diarrheal samples. Multiple bacterial pathogens were found 10 times more commonly in diarrheal than in nondiarrheal samples (20 versus 2%; P < 0.001).

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Section: Discussioncontrasting

confidence: 56%

Prevalence of Diarrheagenic Escherichia coli in Finns with or without Diarrhea during a Round-the-World Trip

Keskimäki

Mattila

Peltola³

et al. 2000

J Clin Microbiol

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“…While EAggEC was the commonest pathogen in three studies, 2,6,20 it was not investigated in one study. 13 In the work carried out by Aranda et al, 21 the commonest pathogen was EPEC. In the present study, the commonest E. coli was EAggEC.…”

Section: Discussionmentioning

confidence: 98%

“…Multiplex PCR using published primers (Appendix 1 [8][9][10][11][12][13][14][15] ) was performed in a total reaction volume of 50 mL (Master mix -12.5 mL, reverse and forward oligonucliotide primers -50 pmol, and boiled suspension as template DNA -6.0 mL). The amplification was performed in thermal mini cycler (M. J.…”

Section: Detection Of Diarrhoeagenic E Coli By Polymerase Chain Reacmentioning

confidence: 99%

Role of Escherichia coli in acute diarrhoea in tribal preschool children of central India

Anvikar

Dolla

Rao

et al. 2007

Paediatric Perinatal Epid

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Five hundred and eighty preschool children belonging to tribal areas of Madhya Pradesh were followed up daily for the presence of diarrhoea for a period of 1 year. In all, 1236 episodes of diarrhoea were recorded with an average of 2.13 episodes per child per year. Stool samples were collected during 780 episodes. They were cultured to isolate Escherichia coli as well as non-E. coli enteropathogens. Ten different genes were detected to identify all diarrhoeagenic E. coli using multiplex polymerase chain reaction. Enteroaggregative E. coli was the commonest diarrhoeagenic E. coli and was isolated from 64 children, followed by enteropathogenic E. coli (27), enterotoxigenic E. coli (10) and enterohaemorrhagic E. coli (2). Other enteropathogens detected were bacteria such as Shigella sp, Vibrio cholerae and Salmonella sp, parasites such as Giardia lamblia and Entameba histolytica, and rotavirus. Most of the bacterial pathogens were multi-drug resistant. The study shows that diarrhoeagenic E. coli contribute significantly to the burden of acute diarrhoea in tribal preschool children.

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“…Attaching and effacing gene (eae 790 basepairs [bp]) and bundle-forming pilus gene (bfpA 324 bp) were identified for EPEC. 9,10 Attaching and effacing gene (eae 790 bp), shiga toxin gene (stx 900 bp) and enterohemolysin gene (ehxA 321 bp) were identified for EHEC. 9,11,12 To detect and identify the stx gene, we used the Lin-all system PCR, followed by digestion with Hinc II to further identify stx1 and stx2.…”

Section: Methodsmentioning

confidence: 99%

Etiology of Acute Diarrhea in Children and Adults in Tunis, Tunisia, with Emphasis on Diarrheagenic Escherichia coli: Prevalence, Phenotyping, and Molecular Epidemiology

et al. 2007

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A total of 271 stool specimens were collected from children (diarrheagenic, n = 115 and control, n = 54) and adults (diarrheagenic, n = 73 and control, n = 29) from Tunis, Tunisia, and processed to detect bacterial enteropathogens, parasites, and viruses. Diarrheagenic Escherichia coli (DEC) were identified by their virulence genes (polymerase chain reaction) and adherence patterns (tissue culture assays). The most frequently isolated enteric pathogens from diarrheagenic children were enterotoxigenic E. coli (ETEC, 32.3%), enteroaggregative E. coli (EAEC, 11.3%), enteroinvasive E. coli (EIEC, (11.3%), adenovirus (10.4%), enterohemorrhagic E. coli (EHEC, 10.4%), and Salmonella spp. (9.5%). For children in the control group, ETEC (37%), EAEC (15%), EHEC (11.1%), and typical enteropathogenic E. coli (EPEC, 11.1%) were the most common enteric pathogens. In adults in the diarrheagenic group, Salmonella spp. (34.2%), ETEC (12.3%), adenovirus (7%), and Shigella spp. (4%) were the most common enteric pathogens. In adults in the control group, ETEC (31%) was the most common enteric pathogen. Multiple pathogens were recovered from 22% of the diarrheagenic children and 7% of the diarrheagenic adults. Escherichia coli strains showed high resistance rates to tetracycline, streptomycin, and beta-lactams. The most frequent combinations were ETEC-rotavirus and ETEC-adenovirus. Pulsed-field gel electrophoresis for DEC indicated a large number of DEC clones (five major clones) persistent in the community reservoir for a considerable period of time that caused diarrhea in the population. This suggests the confluence of small epidemics by clonally related DEC strains circulating in this region.

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Differentiation of pathogenic Escherichia coli strains in Brazilian children by PCR

Cited by 99 publications

References 24 publications

Prevalence of Diarrheagenic Escherichia coli in Finns with or without Diarrhea during a Round-the-World Trip

Prevalence of Diarrheagenic Escherichia coli in Finns with or without Diarrhea during a Round-the-World Trip

Role of Escherichia coli in acute diarrhoea in tribal preschool children of central India

Etiology of Acute Diarrhea in Children and Adults in Tunis, Tunisia, with Emphasis on Diarrheagenic Escherichia coli: Prevalence, Phenotyping, and Molecular Epidemiology

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