Differentiation of Mononuclear Blood Cells into Macrophages, Fibroblasts and Endothelial Cells in Thrombus Organization

Leu, H. J.; Feigl, W; Susani, Martin; Odermatt, Bernhard

doi:10.1159/000163481

Cited by 20 publications

(28 citation statements)

References 12 publications

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“…During the organizing process of a thrombus, fibroblasts migrate into the blood clot, transform into myofibroblasts, form granulation tissue, and finally replace this tissue with solid connective tissue. 17,18 We intended to accelerate this natural response for the healing process of excluded aneurysmal sac. An important point for the organization is that the migrated cells are viable, because administration of materials without viable cells appears to be associated with difficulties in promoting the organization, particularly in the long-term.…”

Section: Discussionmentioning

confidence: 99%

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Development of a New Method for Endovascular Aortic Repair

Kajimoto

Shimono²,

Hirano³

et al. 2006

Circulation

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Background-Endovascular aortic repair by stent grafts (S/Gs) has been developed as a less invasive treatment for aortic aneurysms. However, some aneurysmal cavities can remain without organization, causing re-expansion. We demonstrated previously that transplantation of a cell combination (myoblasts and fibroblasts) promoted thrombus organization in a rat model. We also developed basic fibroblast growth factor (bFGF) slow-delivery S/Gs coated with elastin and impregnated with bFGF. Here, we evaluated the effects of cell transplantation combined with bFGF slow release on canine thoracic aortic aneurysmal sacs after S/Gs repair. Methods and Results-Thoracic aortic aneurysms were surgically created with jugular vein patches in 15 beagles.Myoblasts and fibroblasts of autologous skeletal muscle were isolated and cultured for cell transplantation. The S/Gs had 6 holes and produced endoleaks in the excluded cavities. Collagen gel (gel group, nϭ5) or a mixture of skeletal myoblasts and fibroblasts with collagen gel (cell group, nϭ5) were injected into the aneurysmal sacs excluded by the S/Gs. We also studied the effects of combined therapy of bFGF slow-release S/Gs and cell transplantation (hybrid group, nϭ5). After 14 days, histological analyses revealed that the excluded aneurysmal cavities of the gel group were filled with fresh thrombus, whereas the excluded cavities in the cell-transplanted groups were occupied by organized tissue. The percentages of the organized areas relative to the excluded cavities, evaluated by Masson's trichrome staining, were 18.1Ϯ4.0%, 52.6Ϯ4.0%, and 77.1Ϯ6.9% in the gel, cell, and hybrid groups, respectively. Collagen fibers had already appeared, and increased numbers of ␣-smooth muscle actin-positive cells were observed in the hybrid group. Conclusions-Cell

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Section: Discussionmentioning

confidence: 99%

“…It is well known that myofibroblasts expressing ␣-SMA generate contraction forces and minimize the size of organized tissues. [17][18][19] These results may be said to show the ideal tendencies, such that shrinkage of the aneurysm may be expected later.…”

Section: Discussionmentioning

confidence: 99%

Development of a New Method for Endovascular Aortic Repair

Kajimoto

Shimono²,

Hirano³

et al. 2006

Circulation

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“…9 Indeed, in vitro studies have demonstrated that a specific subset of hematopoietic, pluripotent mononuclear cells is able to differentiate into several phenotypes, including fibroblasts. 10 In addition, platelets and MM adhere on stainless steel, the latter being able to secrete TGF-b1 and PDGF-BB at levels significantly higher than cells adhering on tissue culture plastic. 3,4 In the case of cells isolated from diabetic subjects, MM basal levels of these two growth factors appear to be relatively higher than in those from nondiabetic individuals regardless of the metal substrate challenge.…”

Section: Introductionmentioning

confidence: 98%

Substrate‐induced phenotypical change of monocytes/macrophages into myofibroblast‐like cells: A new insight into the mechanism of in‐stent restenosis

Stewart

Guildford

Lawrence-Watt

et al. 2008

J Biomedical Materials Res

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Stented coronary angioplasty is the procedure of choice to re-establish patency in obstructed coronary arteries. However, the stent implantation procedure often leads to in-stent restenosis, a process that is characterized by stent strut colonization by macrophages and smooth muscle cells and by neointima formation. The present in vitro study investigates the effect of stent materials on the phenotypical features of monocyte/macrophages. Human peripheral blood monocytes from healthy donors (n = 7) were cultured up to 7 days on substrates mimicking: (i) the stent surface (i.e., electropolished stainless steel), (ii) the de-endothelialized vessel wall (collagen-based extracellular matrix gel), and (iii) thrombus (i.e., fibrin gel). The cells were analyzed by immunocytochemistry for their ability to express alpha-actin, a typical myofibroblast marker, by ELISA to determine PDGF-BB and TGF-beta1 secretion and by PCR to evaluate hyaluronan synthase 1, 2, and 3 genes expression. Data were statistically analyzed by ANOVA (Dunnett's test) and data considered significantly different at p

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“…However, thrombosis and corollary inflammation are in fact potent biochemical triggers for fibrosis during vascular remodeling, 15,16 also in the context of endovascular laser-tissue interactions. 17 It is therefore more likely that both processes are required for posttherapeutic vessel closure, whereby thrombosis facilitates acute closure and fibrosis the permanent closure.…”

Section: Sirmentioning

confidence: 99%

Thrombosis versus thermal coagulum formation as a result of endovenous laser treatment: Biochemistry versus photophysics

Heger

2013

Phlebology

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Differentiation of Mononuclear Blood Cells into Macrophages, Fibroblasts and Endothelial Cells in Thrombus Organization

Cited by 20 publications

References 12 publications

Development of a New Method for Endovascular Aortic Repair

Development of a New Method for Endovascular Aortic Repair

Substrate‐induced phenotypical change of monocytes/macrophages into myofibroblast‐like cells: A new insight into the mechanism of in‐stent restenosis

Thrombosis versus thermal coagulum formation as a result of endovenous laser treatment: Biochemistry versus photophysics

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