Differentiation of leptospires of the serogroup Pomona by monoclonal antibodies, pulsed-field gel electrophoresis and arbitrarily primed polymerase chain reaction

Ciceroni, Lorenzo; Ciarrocchi, Simonetta; Ciervo, Alessandra; Petrucca, Andrea; Pinto, Antonella; Calderaro, Adriana; Viani, Isabella; Galati, L.; Dettori, G.; Chezzi, Carlo

doi:10.1016/s0923-2508(01)01284-0

Cited by 19 publications

(15 citation statements)

References 48 publications

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“…However, our use of PFGE and gyrB sequence analysis as alternative methods for typing isolates have been done in other studies of leptospirosis. 6,7,22,26,28,29,38,53 The results of our study provide useful information on the epidemiology of leptospirosis in the Philippines, which until now was not well studied. However, studies with larger sample sizes on leptospirosis among rats, humans, and other animals in other areas of the Philippines would be beneficial in determining the transmission cycle of leptospirosis and the status of this zoonosis.…”

Section: Discussionmentioning

confidence: 90%

Serologic and Molecular Studies of Leptospira and Leptospirosis among Rats in the Philippines

Villanueva¹,

Ezoe²,

Baterna³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. Rats are known to be the most important reservoirs and transmission sources of leptospirosis. However, the status of leptospirosis in the Philippines regarding reservoirs and transmission remains unknown. A survey was conducted in Metro Manila and Laguna that analyzed samples obtained from 106 rats. Using the microscopic agglutination test, we found that 92% of rat serum samples were positive for anti-Leptospira antibodies; the most common infecting serovars were Manilae, Hebdomadis, and Losbanos. On the basis of pulsed-field gel electrophoresis and gyrase B gene sequence analyses, four groups of rat kidney isolates were found: L. interrogans serovar Manilae, serovar Losbanos, and serogroup Grippotyphosa, and L. borgpetersenii serogroup Javanica. Most isolates were lethal after experimental infection of golden Syrian hamsters. Results showed that these four Leptospira serovars and serogroups are circulating among rats, and that these animals may be one of the possible transmission sources of leptospirosis in the Philippines.

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Section: Discussionmentioning

confidence: 90%

Serologic and Molecular Studies of Leptospira and Leptospirosis among Rats in the Philippines

Villanueva¹,

Ezoe²,

Baterna³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…Animal populations of the same species can exhibit either chronic or acute infections with serovar Pomona. Restriction fragment length polymorphism (RFLP) analysis of L. interrogans serovar Pomona isolates has revealed the presence of two major subtypes (5,22) that can be further differentiated by HpaII digestion (2) or IS1500 hybridization (24) patterns. Specific clonal populations, rather than all members of serovar Pomona, may form stable associations with selected maintenance host species (2).…”

mentioning

confidence: 99%

Variable Nucleotide Tandem-Repeat Analysis Revealing a Unique Group of Leptospira interrogans Serovar Pomona Isolates Associated with California Sea Lions

Zuerner

Alt

2009

J Clin Microbiol

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“…A maioria dessas técnicas apresenta limitações de ordem prática resultantes da complexidade, lentidão dos procedimentos para detectar e caracterizar o agente infeccioso ou ainda infraestrutura laboratorial necessária, além das limitações relativas à sensibilidade e especificidade, que são fatores complicadores para a realização de diagnóstico de baixo custo, prático e preciso (CICERONI et al, 2002;MANTEROLA et al, 2003). Assim, torna-se necessário a busca por métodos que apresentem mais especificidade, sensibilidade e rapidez no diagnóstico de bactérias no sêmen de touros a campo e de central de inseminação.…”

Section: Resultado E Discussãounclassified

Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

Dias

Nunes

Cavalcante

et al. 2014

Rev. Acad. Ciênc. Anim.

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Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR) [I] Detection of Histophilus somni (Haemophilus somnus) in bovine semen using polymerase chain reaction (PCR) [a] ResumoO presente estudo avaliou o uso da PCR para a detecção do Histophilus somni no sêmen bovino. Amostras de sêmen foram contaminadas experimentalmente com H. somni diluída em escalas de 10 7 a 10 1 bactérias/ mL, submetidas à extração de DNA pelo método de fenol/clorofórmio e amplificadas pela PCR. Os produtos da amplificação do DNA foram analisados por eletroforese em gel de acrilamida 8%. Por meio de oligonucleotídeos iniciadores obteve-se a amplificação de um fragmento de 400 pares de bases a partir do DNA da bactéria. Conseguiu-se amplificação positiva até na diluição de 10 1 bactérias/mL. A PCR mostrou-se eficiente na detecção de H. somni. O resultado disponibiliza conhecimento relevante para o diagnóstico de H. somni,

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Differentiation of leptospires of the serogroup Pomona by monoclonal antibodies, pulsed-field gel electrophoresis and arbitrarily primed polymerase chain reaction

Cited by 19 publications

References 48 publications

Serologic and Molecular Studies of Leptospira and Leptospirosis among Rats in the Philippines

Serologic and Molecular Studies of Leptospira and Leptospirosis among Rats in the Philippines

Variable Nucleotide Tandem-Repeat Analysis Revealing a Unique Group of Leptospira interrogans Serovar Pomona Isolates Associated with California Sea Lions

Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

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