1995
DOI: 10.1111/j.1348-0421.1995.tb02194.x
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Differentiation of Clostridium difficile, Clostridium bifermentans, Clostridium sordellii, and Clostridium perfringens from Diarrheal Stool by API ZYM and API LRA Oxidase Test

Abstract: A simple, rapid and reliable outline for identification of clostridia isolates from human infections was developed. It consists of a combination of API ZYM and API LRA Oxidase tests. The enzymatic activities were performed with strains sub-cultured onto carbohydrate-free medium (Columbia blood agar). Fifty-five strains of Clostridium difficile, C. bifermentans, C. sordellii, and C. perfringens from clinical specimens and eight reference standard strains representing different species of the same genus were ana… Show more

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Cited by 2 publications
(1 citation statement)
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“…ELISA and PET-RPLA sensitivity rates were only slightly better, detecting 40% and 42% of all enterotoxins, respectively. In a subsequent study, 80 PET-RPLA was compared with a Vero cell assay in a case-control study of a C. perfringens food poisoning outbreak in Italy. The Vero cell assay detected only 8/15 (53.30%) of PCR-confirmed cases, whereas the PET-RPLA could detect 13/15 (86.70%).…”
Section: Antibody-based Testsmentioning
confidence: 99%
“…ELISA and PET-RPLA sensitivity rates were only slightly better, detecting 40% and 42% of all enterotoxins, respectively. In a subsequent study, 80 PET-RPLA was compared with a Vero cell assay in a case-control study of a C. perfringens food poisoning outbreak in Italy. The Vero cell assay detected only 8/15 (53.30%) of PCR-confirmed cases, whereas the PET-RPLA could detect 13/15 (86.70%).…”
Section: Antibody-based Testsmentioning
confidence: 99%