Differentiation of BMMSCs into odontoblast-like cells induced by natural dentine matrix

Lei, Gang; Yu, Yang; Jiang, Yujiao; Wang, Sainan; Yan, Ming; Smith, Anthony J.; Smith, Gay; Cooper, Paul R.; Tang, Chunbo; Zhang, Guangdong; Yu, Jinhua

doi:10.1016/j.archoralbio.2013.01.002

Cited by 24 publications

(19 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Like other tissues, three primary components are required to regenerate a necrotic pulp: 1) vital root canal cells, which can distinguish into normal pulp cells, 2) morphogenic and growth factors to activate and encourage cell distinction, and 3) a matrix that ensures an environment that maintains their vitality and growth and supports cells in a mechanical way 58,59,61,62 .…”

Section: Discussionmentioning

confidence: 99%

“…Across four experiments that were all on subcutaneous transplants used SHEDs 43,58- 60 . Four experiments used BMSCs; two were transplanted to the renal capsule 61,62 , one to the subcutaneous 63 , and the other to the root canal 64 . Three trials tried to regenerate periodontal ligament (PDL) tissue using PDLSCs; two subcutaneously transplanted 41,55 and one transplanted into a socket for extraction 22 .…”

Section: Types Of Stem Cellsmentioning

confidence: 99%

“…Zhang et al 63 proposed applying endogenous BM-MSC to a subcutaneous root canal tooth to a regenerative tissue after the systemic homing in the root canal, driven by the use of the stromal cell-derived factor-1 (SDF-1). The BC, blood clot; bFGF, basic fibroblast growth factor; BMP, bone morphogenetic protein; BMSC, bone marrow mesenchyme stem cell; CC, costal chondrocyte; CDHA, calcium carbonate hydroxyapatite; CNCC, cranial neural crest cell; CXCL14, chemokine (CXC motif) ligand-14; DFSC, dental follicle stem cell; DPSC, dental pulp stem cell; FGF, fibroblast growth factor; GCSF, granulocyte colony-stimulating factor; GF, growth factors; HA, hydroxyapatite; iPS, induced pluripotent stem cell; MCP1, monocyte chemoattractant protein-1; MSC, mesenchymal stem cell; MTA, mineral trioxide aggregate; NA, not exogenously added; ND, not determined; NGF, nerve growth factor; PCL, polycaprolactone; PGA, polyglycolic acid; PGDF, platelet-derived growth factor; PLGA, polylactic-co-glycolic acid; PLLA, poly-L-lactic acid; PRF, platelet-rich fibrin; PRP, platelet-rich plasma; REP, regenerative endodontic procedure; SCAP, stem cell of the apical papilla; SHED, stem cell from human exfoliated deciduous teeth; TCP, tricalcium phosphate; TDM, treated dentin matrix; TGF, transforming growth factor; VEGF, vascular endothelial growth factor; DBCs, dental bud cells use of dentine-matrix-scaffolding cells was associated to the differentiation of the stem cells in a dentinal tubule in polarized odontoblast-like cells 62 .…”

Section: Bone Marrow Derived Mesenchymal Stem Cellsmentioning

confidence: 99%

See 2 more Smart Citations

Dental stem cells in tooth repair: A systematic review

2019

View full text Add to dashboard Cite

Background: Dental stem cells (DSCs) are self-renewable teeth cells, which help maintain or develop oral tissues. These cells can differentiate into odontoblasts, adipocytes, cementoblast-like cells, osteoblasts, or chondroblasts and form dentin/pulp. This systematic review aimed to summarize the current evidence regarding the role of these cells in dental pulp regeneration. Methods: We searched the following databases: PubMed, Cochrane Library, MEDLINE, SCOPUS, ScienceDirect, and Web of Science using relevant keywords. Case reports and non-English studies were excluded. We included all studies using dental stem cells in tooth repair whether in vivo or in vitro studies. Results: Dental pulp stem cell (DPSCs) is the most common type of cell. Most stem cells are incorporated and implanted into the root canals in different scaffold forms. Some experiments combine growth factors such as TDM, BMP, and G-CSF with stem cells to improve the results. The transplant of DPSCs and stem cells from apical papilla (SCAPs) was found to be associated with pulp-like recovery, efficient revascularization, enhanced chondrogenesis, and direct vascular supply of regenerated tissue. Conclusion: The current evidence suggests that DPSCs, stem cells from human exfoliated deciduous teeth, and SCAPs are capable of providing sufficient pulp regeneration and vascularization. For the development of the dental repair field, it is important to screen for more effective stem cells, dentine releasing therapies, good biomimicry scaffolds, and good histological markers.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Types Of Stem Cellsmentioning

confidence: 99%

Section: Bone Marrow Derived Mesenchymal Stem Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Dental stem cells in tooth repair: A systematic review

2019

View full text Add to dashboard Cite

show abstract

“…The differentiation of odontoblasts from cultured stem cells of dental and non-dental origin has made odontoblast-like cells a valuable model for examining the mechanisms regulating the sequential steps involved in odontoblast differentiation (20,11) .…”

Section: Discussionmentioning

confidence: 99%

In Vitro differentiation potential of Isolated dental pulp stem cells

Moussa

Hafez

Sabry

2018

Egyptian Dental Journal

View full text Add to dashboard Cite

Objective: The aim of the present study is to isolate and characterize human dental pulp stem cells (DPSCs).Surface markers expression utilizing surface markers for flow cytometry. The odontogenic differentiation of DPSCs was assessed. Material and methods: dental pulp tissuses were collected from impacted third molars. samples were treated with collagenase before centrifugation to allow release of the cells. Cells were cultured in complete culture medium for 12-14 days. After reaching confluence, the isolated cells were characterized by flow cytometry using CD105 and CD45. The cells were induced for odontogenic differentiation by placing the cells in odontogenic induction media for 14 days. Odontogenic differentiation was evaluated by Alizarin Red stain and by the expressions of dentine sialo phospho protein (DSPP), dentin matrix protein-1 (DMP-1) and alkaline phosphatase (ALP) activity, which were assessed by RT-PCR. Results: The results showed that successful isolation of stem cells from human dental pulp was achieved. Cells were successfully sub-cultured and expanded up to the third passage. A flow cytometric analysis was done after stem cells isolation. Isolated DPSCs were identified by positive expression of CD105 and negative expression of hematopoietic marker C. The results showed that differentiatiationed odontoblastic like cells from DPSCs induced by odontogenic medium were stained by Alizarin Red at days 7 and 14. RT-PCR results indicated that all cultured cells efficiently differentiate into dentin forming cells and expressed specific DSPP, DMP1 and ALP genes at days 7 and 14. Conclusion: DPSCs can be a possible source of stable differentiated odontoblastic cells. Their ability of inducing hard tissue formation offer an alternative method to save teeth with compromised structural integrity.

show abstract

“…But the process of scaffold material degradation might lead to the host's immune reaction and toxic reaction for seed cells (Hofmann et al., ). The decalcified dentin matrix (DDM), which originates from the extracted third molar or orthodontic teeth, can induce cell differentiation (Lei et al., ). It has good biocompatibility, bone conduction and bone induction properties, and it is a type of natural porous and slow‐releasing stent material (Gomes, Abreu, Morosolli, Araujo, & Goulart, ).…”

Section: Introductionmentioning

confidence: 99%