Differentiation between Bulinus truncatus and Bulinus hexaploidus by morphological characters, chromosomal study and compatibility with Schistosoma haematobium

Mansour, Shereen Mahfouz; Ibrahim, Amina M.

doi:10.1016/j.exppara.2023.108502

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…In the present data, the mother sporocysts reproduce several daughters' sporocysts and migrate to the digestive gland of the snails at 21 DAE. This result is in agreement with Mansour and Ibrahim (2023). After the invasion of miracidia and during the sporocyst development in the snail tissues, the parasite has to interact with the snail immune system as the presence of an extracellular matrix that surrounds and envelops the sporocysts (Van der Knaap & Loker, 1990).…”

Section: Discussionsupporting

confidence: 93%

“…The main function of the hemocytes is to freely circulate in tissues and hemolymph to recognize and phagocytose non‐self‐particles (Van der Knaap & Loker, 1990) or to recognize and destroy the parasite through encapsulation reaction involving plasma activating or recognition factors, lysosomal enzymes, or other cytotoxic elements and phagocytosis of the damaged parasite tegument followed by degeneration and finally exclusion from the tissues of the snails (Mansour & Ibrahim, 2023). The present results indicated that the hemocytes were aggregated around the sporocysts.…”

Section: Discussionmentioning

confidence: 99%

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Molecular detection of Schistosoma haematobium in infected Bulinus truncatus snails associated with immune response

Mansour,

El Einin,

Tadrous

et al. 2023

Microscopy Res & Technique

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Schistosomiasis is one of the most common waterborne parasite illnesses, it is a major public health issue in developing countries. The polymerase chain reaction (PCR) technique is used to find Schistosoma haematobium DNA in Bulinus truncatus, which could speed up the discovery of infections before cercariae are shed. DraI‐PCR detected S. haematobium infection at different infection intervals with bands at 300 bp in shedding snails 40 days after exposure and even on the first day after B. turancuts snails exposure to miracidia. Transmission electron microscopy showed the structure of sporocyst from 1 to 40 days post‐exposure and activated hemocytes in infected non‐shedding snails as well as sporocyst degradation. Flow cytometry was used to measure the percentage of Bax and TGF‐β1 positive stained cells that have been linked with infection progression. In conclusion, molecular tools and immune response play an important role in the strategy of controlling schistosomiasis through the early detection of larval stages in intermediate hosts toward certification of schistosomiasis elimination.Research Highlights DraI‐PCR allowed early detection of S. haematobium at 300 bp in B. truncatus snail. Transmission electron microscopy showed the structure of S. haematobium sporocyst in snail and activated hemocytes in non‐shedding snail. Bax protein that induced apoptotic changes and Transforming Growth Factor Beta1 level have been linked with parasite development.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Molecular detection of Schistosoma haematobium in infected Bulinus truncatus snails associated with immune response

Mansour,

El Einin,

Tadrous

et al. 2023

Microscopy Res & Technique

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“…This disease is prevalent in tropical and subtropical areas, affecting approximately 240 million people globally, with about 700 million people at risk, particularly in poor communities with inadequate sanitation facilities [ 3 – 6 ]. Schistosoma mansoni and S. haematobium are the two parasites that cause the most widespread forms of intestinal and urogenital schistosomiasis [ 7 ]. In our laboratory, we use the infection of B. alexandrina with S. mansoni and B. truncatus with S. haematobium to study the impact of host-parasite infections on physiological and behavioral changes, including reduced fecundity and increased feeding behavior in the two intermediate host species.…”

Section: Introductionmentioning

confidence: 99%

Impact of Schistosoma sp., Infection on Biological, Feeding, Physiological, Histological, and Genotoxicological Aspects of Biomphalaria alexandrina and Bulinus truncatus Snails

Dokmak,

Hammam,

Ibrahim

2024

Acta Parasit.

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Background Trematode infections of the genus Schistosoma can induce physiological and behavioral changes in intermediate snail hosts. This is because the parasite consumes essential resources necessary for the host's survival, prompting hosts to adapt their behavior to maintain some level of fitness before parasite-induced mortality occurs. Methods In this study, the reproductive and biochemical parameters of Biomphalaria alexandrina and Bulinus truncatus were examined during the cercareal shedding stage of infection with Schistosoma mansoni and Schistosoma haematobium, respectively, compared with controls. Results The study revealed an infection rate of 34.7% for S. mansoni and 30.4% for S. haematobium. In B. alexandrina infected with S. mansoni, a survival rate of 65.2% was recorded, along with a mean prepatent period of 30.3 ± 1.41 days, a mean shedding duration of 14.2 ± 0.16 days, and a mean lifespan of 44.1 ± 0.24 days. Meanwhile, in B. truncatus infected with S. haematobium, a survival rate of 56.4% was observed, with a mean prepatent period of 44.3 ± 1.41 days, a mean shedding duration of 22.6 ± 2.7 days, and a mean lifespan of 66.9 ± 1.6 days. Feeding increased in both infected species of snails, while the net reproductive rate (Ro) of the infected snails decreased. Total antioxidant (TAO) and lipid peroxidation activity increased in the two infected snail species during shedding, while Glutathione-S-transferase levels decreased. Lipid peroxidase activity and nitrogen oxide levels significantly decreased in infected B. alexandrina and increased in infected Bulinus. Steroid hormone levels were elevated in infected Biomphalaria, whereas they were reduced in infected Bulinus. Comet assay parameters showed an increase in the two infected genera after infection compared to control snails, indicating genotoxic damage and histopathological damage was observed. Conclusions These findings demonstrate that infection with larva species diverse biochemical, hormonal, genotoxic, and histopathological changes in the tissues responsible for fecundity and reproduction in B. alexandrina and B. truncates comparing with controls.

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Laevicaulis stuhlmanni slugs as accumulation bio-indicators of lead metal pollution: immunotoxic, physiological, and histopathological alterations

Ibrahim,

Mobarak

2024

Environ Sci Pollut Res

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Differentiation between Bulinus truncatus and Bulinus hexaploidus by morphological characters, chromosomal study and compatibility with Schistosoma haematobium

Cited by 5 publications

References 48 publications

Molecular detection of Schistosoma haematobium in infected Bulinus truncatus snails associated with immune response

Molecular detection of Schistosoma haematobium in infected Bulinus truncatus snails associated with immune response

Impact of Schistosoma sp., Infection on Biological, Feeding, Physiological, Histological, and Genotoxicological Aspects of Biomphalaria alexandrina and Bulinus truncatus Snails

Laevicaulis stuhlmanni slugs as accumulation bio-indicators of lead metal pollution: immunotoxic, physiological, and histopathological alterations

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