Differentiating vitreous proteomes in proliferative diabetic retinopathy using high-performance liquid chromatography coupled to tandem mass spectrometry

Wang, Hao; Li, Feng; Hu, Jiayin; Xie, Chunlei; Wang, Fang

doi:10.1016/j.exer.2012.11.023

Cited by 57 publications

(76 citation statements)

References 38 publications

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“…Thus, proteolytic processing of CCN1 by MMPs potentially contributed to the enrichment of vitreal fluids with CCN1 truncated forms. The levels of antiangiogenic growth factors such as angiopoietin-2 were dramatically diminished (data not shown), which is consistent with previous reports (38).…”

Section: Ccn1 Degradome Products In Vitreal Fluids-ccn1supporting

confidence: 82%

Degradome Products of the Matricellular Protein CCN1 as Modulators of Pathological Angiogenesis in the Retina

Choi

Lin

Shrier

et al. 2013

Journal of Biological Chemistry

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Section: Ccn1 Degradome Products In Vitreal Fluids-ccn1supporting

confidence: 82%

Degradome Products of the Matricellular Protein CCN1 as Modulators of Pathological Angiogenesis in the Retina

Choi

Lin

Shrier

et al. 2013

Journal of Biological Chemistry

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“…Mass spectrometry (MS)‐based quantitative proteomics provides a means for the determination of global proteome changes at the tissue and cellular levels, enabling a molecular level characterization of the pathophysiologies of complex eye disorders. Currently, most proteomic studies characterizing disease‐induced vitreous proteome changes have focused on proliferative and nonproliferative diabetic retinopathies (Kim et al, 2007; Loukovaara et al, 2015; Wang, Feng, Hu, Xie, & Wang, 2013), proliferative vitreoretinopathy (Garweg, Tappeneiner, & Halberstadt, 2013; Mitry, Fleck, Wright, Campbell, & Charteris, 2010), and age‐related macular degeneration (AMD; Koss et al, 2014), whereas iERM and MH remain less‐studied (Mandal et al, 2013; Pollreisz et al, 2013; Yu et al, 2014; Zhang et al, 2017). …”

Section: Introductionmentioning

confidence: 99%

Systems pathology analysis identifies neurodegenerative nature of age‐related vitreoretinal interface diseases

et al. 2018

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Aging is a phenomenon that is associated with profound medical implications. Idiopathic epiretinal membrane (iEMR) and macular hole (MH) are the major vision‐threatening vitreoretinal diseases affecting millions of aging people globally, making these conditions an important public health issue. iERM is characterized by fibrous tissue developing on the surface of the macula, which leads to biomechanical and biochemical macular damage. MH is a small breakage in the macula and is associated with many ocular conditions. Although several individual factors and pathways are suggested, a systems pathology level understanding of the molecular mechanisms underlying these disorders is lacking. Therefore, we performed mass spectrometry‐based label‐free quantitative proteomics analysis of the vitreous proteomes from patients with iERM and MH to identify the key proteins, as well as the multiple interconnected biochemical pathways, contributing to the development of these diseases. We identified a total of 1,014 unique proteins, many of which are linked to inflammation and the complement cascade, revealing the inflammation processes in retinal diseases. Additionally, we detected a profound difference in the proteomes of iEMR and MH compared to those of diabetic retinopathy with macular edema and rhegmatogenous retinal detachment. A large number of neuronal proteins were present at higher levels in the iERM and MH vitreous, including neuronal adhesion molecules, nervous system development proteins, and signaling molecules, pointing toward the important role of neurodegenerative component in the pathogenesis of age‐related vitreoretinal diseases. Despite them having marked similarities, several unique vitreous proteins were identified in both iERM and MH, from which candidate targets for new diagnostic and therapeutic approaches can be provided.

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“…This also poses a problem in acquiring samples for study purposes since ethically healthy eyes cannot be subjected to the procedures for the sole purpose of extracting the fluid [1]. To address this issue, researchers have used samples of patients undergoing eye surgery for various diseases but that are presumed to have healthy vitreous humor as controls [25,26] while others have used corneal transplant donated eyes [27,28]. The first study conducted with corneal transplant donated eyes as controls carried out with type 2 diabetes patients [27] revealed 29 differentially expressed proteins: eight of which were increased and 21 which were decreased in proliferative diabetic retinopathy (PDR) patients.…”

Section: Vitreous Humormentioning

confidence: 99%

“…The pathways associated with PDR have also been studied with quantitative proteomic techniques [28]. Among their findings, they identified differentially expressed proteins involved in glycolysis/gluconeogenesis, complement and coagulation cascades, gap junction, and phagosome pathways.…”

Section: Vitreous Humormentioning

confidence: 99%

A Quantitative Proteomics Approach to Clinical Research with Non-Traditional Samples

2016

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The proper handling of samples to be analyzed by mass spectrometry (MS) can guarantee excellent results and a greater depth of analysis when working in quantitative proteomics. This is critical when trying to assess non-traditional sources such as ear wax, saliva, vitreous humor, aqueous humor, tears, nipple aspirate fluid, breast milk/colostrum, cervical-vaginal fluid, nasal secretions, bronco-alveolar lavage fluid, and stools. We intend to provide the investigator with relevant aspects of quantitative proteomics and to recognize the most recent clinical research work conducted with atypical samples and analyzed by quantitative proteomics. Having as reference the most recent and different approaches used with non-traditional sources allows us to compare new strategies in the development of novel experimental models. On the other hand, these references help us to contribute significantly to the understanding of the proportions of proteins in different proteomes of clinical interest and may lead to potential advances in the emerging field of precision medicine.

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Differentiating vitreous proteomes in proliferative diabetic retinopathy using high-performance liquid chromatography coupled to tandem mass spectrometry

Cited by 57 publications

References 38 publications

Degradome Products of the Matricellular Protein CCN1 as Modulators of Pathological Angiogenesis in the Retina

Degradome Products of the Matricellular Protein CCN1 as Modulators of Pathological Angiogenesis in the Retina

Systems pathology analysis identifies neurodegenerative nature of age‐related vitreoretinal interface diseases

A Quantitative Proteomics Approach to Clinical Research with Non-Traditional Samples

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