Differentially-expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis

Abstract

Psoriasis is a T cell-mediated, chronic, relapsing and inflammatory cutaneous disorder. The dysfunctional activity of T cells in patients with psoriasis is attributed to bone marrow hematopoietic stem cells (BMHSCs). To understand the pathogenic roles of BMHSCs in psoriasis, a differential gene expression analysis was performed using suppression subtractive hybridization of the BMHSCs from a patient with psoriasis and a healthy control. Using a cDNA array dot blot screening to screen 600 genes from forward- and reverse-subtracted cDNA libraries, 17 differentially-expressed sequence tags (ESTs) were identified. The genes within the ESTs were observed to be the homologs of genes that are involved in various cellular processes, including hormone signaling, RNA catabolism, protein ADP DNA base melting, transcriptional regulation, cell cycle regulation and metabolism. CD45, which was overexpressed in the psoriatic BMHSCs, was further analyzed using relative quantitative polymerase chain reaction. In addition, the levels of CD45 in the peripheral blood cells (PBCs) of the patients with psoriasis were markedly increased and closely associated with disease severity. An abnormality of hematopoietic progenitor cells, e.g., CD45 overexpression, may be transferred to PBCs via hematopoiesis, and may account for the psoriasis-inducing properties of activated T cells.

Therapeutic potential of adipose tissue‐derivatives in modern dermatology

“…Moreover, the observation that TNF‐α inhibitors reduce the expression of several Th1/Th17 cytokines (IL‐6, IL‐8, IL‐12, IL‐23A, IL‐17C, IL‐17F, IL‐21, TNF‐α, CCL2, CCL20, CXCL2, CXCL5, G‐CSF and IFN‐γ) in cutaneous MSCs reverting the disease‐specific phenotype consolidates the idea that MSCs have a specific disease commitment 141–143 . Also, BMSCs from psoriatic patients demonstrated irregular cytokines and growth factors production reduced the ability to suppress overactive immune cells compared with healthy donors 140,144,145 . So, the clinical application of MSCs in psoriatic patients has been sporadically proposed.…”

Benchmarking Stem Cells and Transplantation in Psoriasis

“…Both, genetic and biological profiles were further screened revealing an aberrant activity of bmHSCs with an increased apoptotic behavior [29]. In 2014, Zhang and colleagues reported an overexpression of a differentiation marker of bone marrowderived T-cell progenitor in psoriatic BmHSCs and that this marker maintained higher levels also in peripheral blood cells (PBCs), which were correlated to the severity of the disease [30]. These findings strengthened the theory on the relation between psoriasis abnormalities and hematopoiesis.…”

Role of mesenchymal stem cells in the pathogenesis of psoriasis: current perspectives

“…The altered activity of T-cells in psoriatic patients is attributed by several authors to BMHSCs. Zhang et al 91 performed a differential gene expression analysis by suppression subtractive hybridization of the BMHSCs from a patient with psoriasis and a healthy control. They identified 17 differentially expressed sequence tags (ESTs).…”

“…They speculated that hematopoiesis may transfer the defects of hematopoietic progenitor cells, eg, CD45 overexpression, to PBCs and that this could promote the psoriasis-inducing properties of activated T-cells. 91 …”