Differential Two-Dimensional Protein Patterns as Related to Tissue Specificity and Water Conditions in <i>Brassica napus</i> var <i>oleifera</i> Root System

Damerval, Catherine; Vartanian, Nicole; Vienne, Dominique de

doi:10.1104/pp.86.4.1304

Cited by 25 publications

(12 citation statements)

References 18 publications

(33 reference statements)

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“…Decreases in polysome content and protein synthesis activity probably are global responses to reduced growth rates. In addition, specific changes in poly(A) RNA populations and protein synthesis are observed in plants exposed to low water potentials [2,13,14,15,24,42,45,50]. Water deficit-induced changes in the abundance of selected transcripts and proteins suggest that specific biochemical responses of plants to dehydration involve changes in gene expression.…”

Section: Discussionmentioning

confidence: 99%

Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted. Sequence and expression of three inducible genes

1990

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Reduction of turgor in pea shoots caused the accumulation of several poly(A) RNAs. cDNA clones derived from three different poly(A) RNAs which accumulate in wilted pea shoots were isolated, sequenced and expression of the corresponding genes examined. Clone 7a encoded a 289 amino acid protein. The C-terminal 180 amino acids of this protein were homologous to soybean nodulin-26. RNA hybridizing to cDNA 7a was abundant in roots, and induced in shoots by dehydration, heat shock and to a small extent by ABA. Hydropathic plots indicate that the protein encoded by cDNA 7a contains six potential membrane spanning domains similar to proteins which form ion channels. Clone 15a encoded a 363 amino acid protein with high homology to cysteine proteases. RNA hybridizing to cDNA 15a was more abundant in roots than shoots of control plants. Dehydration of pea shoots induced cDNA 15a mRNA levels whereas heat shock or ABA treatment did not. Clone 26g encoded a 508 amino acid protein with 30% residue identity to several aldehyde dehydrogenases. RNA hybridizing to cDNA 26g was induced by dehydration of shoots but not roots and heat shock and ABA did not modulate RNA levels. Levels of the three poly(A) RNAs increased 4-6-fold by 4 h after wilting and this increase was not altered by pretreatment of shoots with cycloheximide. When wilted shoots were rehydrated, RNA hybridizing to cDNA 26g declined to pre-stress levels within 2 h. Run-on transcription experiments using nuclei from pea shoots showed that transcription of the genes which encode the three poly(A) RNAs was induced within 30 min following reduction of shoot turgor. One of the genes showed a further increase in transcription by 4 h after dehydration whereas transcription of the other 2 genes declined. These results indicate that plant cells respond to changes in cell turgor by rapidly increasing transcription of several genes. Furthermore, the expression of the turgor-responsive genes varies with respect to the time course of induction and reversibility of the wilting-induced changes.

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Section: Discussionmentioning

confidence: 99%

Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted. Sequence and expression of three inducible genes

1990

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“…The 22-kD polypeptides were not detected in well-watered or drought-stressed roots (Fig. 4, L and M) (8).…”

Section: Accumulation Of Two Major 22-kd Polypeptides Undermentioning

confidence: 92%

Characterization of a Novel Protein Induced by Progressive or Rapid Drought and Salinity in Brassica napus Leaves

Reviron¹,

Vartanian²,

Sallantin³

et al. 1992

Plant Physiol.

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Under progressive drought stress, Brassica napus displays differential leaf modifications. The oldest leaves, developed before the onset of water deficit, wilt gradually, whereas the youngest leaves harden. Hardening was distinguished by leaf turgor and bluish wax bloom when the shoot water potential was below -3 MPa and the leaf water saturation deficit was about 60%. This adaptive change was accompanied by modifications in two-dimensional protein profiles. Ten percent of the polypeptides had altered abundance or were unique to drought-stressed plants. Two-dimensional analysis of in vitro translation products did not reveal a general decrease in mRNA population. A 22-kD double polypeptide was increased by progressive or rapid water stress and salinity and disappeared upon rehydration. These polypeptides have a common N-terminal sequence, which does not reveal homology with any known waterstress protein but which contains the signature motif of soybean Kunitz trypsin inhibitors. Immunoprecipitation allowed these polypeptides to be identified on two-dimensional gels of in vitro translation products. They appeared to be synthesized as a 24-kD precursor, and their transcript was present in the control wellwatered leaves, where the polypeptides were never detected, indicating a possible translational regulation. A putative function of this protein, named BnD22, in the retardation of drought-induced leaf senescence is discussed.Rapeseed (Brassica napus L. var oleifera) is often subjected to various environmental stresses (cold, flooding, salinity, etc.). In humid and temperate areas, drought periods are a major factor impeding plant growth and development. This species, like other Cruciferae species, displays an original adaptive strategy. When subjected to progressive drought stress, a dramatic alteration of its root system occurs, characterized by the emergence of numerous roots that remain short, hairless, and often tuberized (3). These roots are able to survive extreme soil desiccation and rapidly recover hair formation and elongation upon rehydration. A specific be-

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“…Differential genome expression in tissues and organs according to developmental or physiological stage has been studied (Klose 1982;Zivy et al 1984;Leonardi et al 1988). The modification of this expression by environmental stresses (e.g., heat shock and water deficit) has also been investigated (Baszczynski and Walden 1982;Zivy 1987;Damerval et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphism in maritime pine (Pinus pinaster Ait.) assessed by two-dimensional gel electrophoresis of needle, bud, and pollen proteins

Bahrman

Petit

1995

J Mol Evol

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Using two-dimensional polyacrylamide gel electrophoresis, the genetic variation of proteins was examined in three organs (needle, bud, and pollen) from 18 trees of maritime pine. Three types of variation were noted: presence/absence, staining intensity, and position variation of the spots. Of the 902 polypeptides scored in the three organs, 245 (27.2%) were polymorphic. Moreover, among these variable spots, 117 were found in a single organ, demonstrating an increased polymorphism of the organ-specific polypeptides (56.0% vs 18.4% for the organ-unspecific polypeptides). Finally, a positive correlation was found between variability level and subunit molecular weight for spots showing position variation but not for spots showing presence/absence or staining intensity variations. Possible explanations for this observation are discussed.

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Differential Two-Dimensional Protein Patterns as Related to Tissue Specificity and Water Conditions in Brassica napus var oleifera Root System

Cited by 25 publications

References 18 publications

Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted. Sequence and expression of three inducible genes

Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted. Sequence and expression of three inducible genes

Characterization of a Novel Protein Induced by Progressive or Rapid Drought and Salinity in Brassica napus Leaves

Genetic polymorphism in maritime pine (Pinus pinaster Ait.) assessed by two-dimensional gel electrophoresis of needle, bud, and pollen proteins

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