Genetic polymorphism in maritime pine (Pinus pinaster Ait.) assessed by two-dimensional gel electrophoresis of needle, bud, and pollen proteins

Abstract

Using two-dimensional polyacrylamide gel electrophoresis, the genetic variation of proteins was examined in three organs (needle, bud, and pollen) from 18 trees of maritime pine. Three types of variation were noted: presence/absence, staining intensity, and position variation of the spots. Of the 902 polypeptides scored in the three organs, 245 (27.2%) were polymorphic. Moreover, among these variable spots, 117 were found in a single organ, demonstrating an increased polymorphism of the organ-specific polypeptides (56.0% vs 18.4% for the organ-unspecific polypeptides). Finally, a positive correlation was found between variability level and subunit molecular weight for spots showing position variation but not for spots showing presence/absence or staining intensity variations. Possible explanations for this observation are discussed.

Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis

“…It has been shown that organ-specific proteins exhibit greater genetic variation than organ-nonspecific proteins (Bahrman and Petit 1995). Our present results confirmed this conclusion.…”

THE UTILITY OF PROTEOMICS IN ALGAL TAXONOMY: BOSTRYCHIA RADICANS/B. MORITZIANA (RHODOMELACEAE, RHODOPHYTA) AS A MODEL STUDY1

“…Several studies have demonstrated that organ‐specific proteins are more variable between genotypes than organ‐unspecific proteins, and that the level of genetic variability depends on the organ or tissue considered. In maritime pine, Bahrman and Petit (1995) examined the genetic variation of proteins in three organs (needle, bud, and pollen) of 18 unrelated trees. Of 902 polypeptides scored for their presence/absence variations, staining intensities, and position shift variations, 27.2% were polymorphic.…”

Comparison of genetic differentiation in maritime pine (Pinus pinaster Ait.) estimated using isozyme, total protein and terpenic loci

“…In general using this technique allowed more than 100 heterozygous loci to be detected in a parental tree. Only 20 per cent of this variation corresponds to isoelectric point variants (allelic variation of structural genes) and 80 per cent (Bahrman & Petit, 1995). Here we scored only 16 loci for which we had studied the segregation using megagametophytes from a single tree (Bahrman & Damerval, 1989).…”